Aquaporin‐1 expression in fluoro‐edenite‐induced mesothelioma effusions: An approach by cell‐block procedure

Angelico, Giuseppe; Ieni, Antonio; Caltabiano, Rosario; Zeppa, Pio; Tuccari, Giovanni

doi:10.1111/cyt.12583

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…Also, cells of a malignant mesothelioma are similar to reactive mesothelial cells, thus cell volume, cluster, nuclear, and cytoplasm findings, as well as evidence of a cellular inter-association are needed for a definitive diagnosis [7]. In addition, an immunocytochemical method the uses a cell transfer process and produces cell blocks is desirable for cytological diagnosis [8,9]. Following are details regarding the cytological morphology of a malignant mesothelioma, as well as immunocytochemical markers useful for differentiation from other malignant tumors and reactive mesothelial cells.…”

Section: General Cytological Features Of Epithelioid Type Of Mpmmentioning

confidence: 99%

Malignant mesothelioma: Cytological features

Okimura¹

2022

Open J Clin Med Images

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A malignant mesothelioma arises from the serosal surface of the pleural, peritoneal, or pericardial cavity. Although rare, with development of diagnostic techniques, the frequency of this tumor has been increasing. Malignant mesothelioma is classified into three types; epithelioid, sarcomatoid, and biphasic. It is especially important to differentiate epithelioid type from reactive mesothelial cells and another type of carcinoma, with a definitive diagnosis usually obtained based on examination of tissue biopsy or surgical resection specimens.

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Section: General Cytological Features Of Epithelioid Type Of Mpmmentioning

confidence: 99%

Malignant mesothelioma: Cytological features

Okimura¹

2022

Open J Clin Med Images

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“…Different diagnostic immunohistochemical markers are currently available for MPM, such as calretinin, CK5/6, podoplanin, mesothelin, osteopontin, hyaluronic acid, fibulin-3 and vascular endothelial growth factor [ 3 ]. In actual fact, tumor stage, histological subtype, sex and age at diagnosis represent the most important prognostic parameters in MPM patients [ 17 ]. Recently, some authors have also emphasized the prognostic and predictive role of the water channel protein aquaporin-1 (AQP1) in MPM patients [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…In actual fact, tumor stage, histological subtype, sex and age at diagnosis represent the most important prognostic parameters in MPM patients [ 17 ]. Recently, some authors have also emphasized the prognostic and predictive role of the water channel protein aquaporin-1 (AQP1) in MPM patients [ 17 , 18 ]. However, reliable prognostic and predictive biomarkers for improving MPM patient management, are yet to be discovered.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Expression of Serine and Arginine-Rich Splicing Factor 1 (SRSF1) in Fluoro-Edenite-Induced Malignant Mesothelioma: A Preliminary Study

Broggi

Angelico

Filetti

et al. 2021

IJERPH

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The Serine and Arginine-Rich Splicing Factor 1 (SRSF1) has a proto-oncogenic function, being associated with angiogenesis and frequently overexpressed in many human malignant neoplasms. Its immunohistochemical expression has never been investigated in malignant pleural mesothelioma (MPM). We evaluated SRSF1 immunoexpression and its possible relation to angiogenesis in a selected cohort of 10 fluoro-edenite(FE)-induced MPM cases. Methods: Immunohistochemical analyses with an anti-SRSF1 antibody were performed. We interpreted the cases as positive if tumor cell nuclei were stained; a semi-quantitative analysis of the cases was performed by evaluating the intensity of staining and the percentage of tumor positive cells. A microvessel density (MVD) count was also performed. Results: High and low immunoexpressions of SRSF1 were seen in six and four MPMs, respectively. A trend of shorter overall survival was found in FE-induced MPM patients with SRSF1 overexpression. In addition, a significant association between high-MVD and high SRSF1 immunoexpression (p = 0.0476) was found. Conclusions: SRSF1 appears to be involved in MPM pathogenesis and its immunoexpression may represent a prognostic biomarker capable of identifying subgroups of patients with different prognosis. However, given the preliminary nature of the present study, further investigations on larger series, and additional in vitro studies, are required to validate our findings.

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“…Their results also confirm the improved diagnostic performance of cell blocks for the cytologic diagnosis of serous effusions, emphasizing that cellularity and atypical cellular groups are better appreciated in cell blocks compared with ThinPrep and liquid‐based cytology. This is mostly attributed to the fact that cell block techniques allow preservation of the cellular architecture and are better suited for immunohistochemical stains through a histological approach 17,18 …”

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confidence: 99%

“…This is mostly attributed to the fact that cell block techniques allow preservation of the cellular architecture and are better suited for immunohistochemical stains through a histological approach. 17,18 In their study, Davis et al highlight the significance of peritoneal cytology for EC, emphasizing the importance of cytohistologic correlations and the role of expert consultation/pathology review as important aspects in patient care. Nonetheless, the authors note that further studies are needed to determine the role of ancillary molecular diagnostics in the reporting and classification of EC from PW specimens.…”

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confidence: 99%

The role of cytology in endometrial cancer: Diagnostic and clinical considerations from peritoneal/pelvic washings. Is it still a heated debate?

Angelico

Santoro

Rossi

et al. 2021

Cancer Cytopathology

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The diagnostic and clinical interpretation of peritoneal/pelvic washing (PW) cytology in endometrial carcinoma (EC) remain controversial. 1-5 The incidence of malignant cytological diagnoses varies widely in different studies ranging from 4.9% to 40%, mostly due to the use of different diagnostic criteria and different thresholds for making a specific diagnosis. 1-5 Moreover, the clinicopathological significance of positive results, especially in women affected by early stage EC, remains an open debate. 1-5 While the role of cytology has been clearly defined for ovarian cancer, its prognostic significance for EC and its exact role in guiding treatment decision making are problematic. 1-5 In this issue of Cancer Cytopathology, Davis et al 6 discuss their diagnostic experience and technical considerations for the use of peritoneal/pelvic cytology in EC patients, raising the unsolved question of both prognostic and staging roles of cytology in EC, particularly in the absence of other evidence of extrauterine spread. 1,2,6 EC is the most common gynecologic cancer in developed countries and, to date, represents a heterogeneous group of diseases with different morphological and molecular features. 7 According to the Cancer Genome Atlas model, 4 molecular categories of EC are recognized: 1) ultramutated (DNA polymerase epsilon mutated), 2) hypermutated secondary to microsatellite instability, 3) low copy number, and 4) high copy number (serous-like). 8 Despite recent advances in defining EC, more than 20% of women with EC still experience recurrences and metastases and eventually die of disease. 9,10 Together with the known biopathological parameters (lymph node status, histological type and grade, stage of disease, depth of myometrial invasion, lymphovascular space involvement, cervical involvement, microcystic elongated and fragmented-MELF pattern of myometrial invasion), the adoption of molecular diagnostic categories in routine clinical practice and the evaluation of WT1 and L1CAM immunohistochemical expression have recently emerged as promising prognostic factors in EC patients. 1,10-13 According to many experts, malignant cytology is associated with adverse outcome only when accompanied by extrauterine spread of the disease. 3,4 However, a positive cytological diagnosis may be considered an indicator of aggressive tumor behavior being related to other adverse prognostic factors, such as high-grade disease, nonendometrioid histology, deep myometrial invasion, lymphovascular space invasion, and lymph node metastases. 1-5 In the study by Davis et al, cytological specimens (both ThinPrep and cell block preparations) from PW of EC patients were retrospectively reviewed and analyzed according to the new diagnostic criteria proposed

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Aquaporin‐1 expression in fluoro‐edenite‐induced mesothelioma effusions: An approach by cell‐block procedure

Abstract: AQP-1 high expression is detectable on cytological samples of FE-induced MM with a prognostic value.

Cited by 7 publications

References 34 publications

Malignant mesothelioma: Cytological features

Malignant mesothelioma: Cytological features

Immunohistochemical Expression of Serine and Arginine-Rich Splicing Factor 1 (SRSF1) in Fluoro-Edenite-Induced Malignant Mesothelioma: A Preliminary Study

The role of cytology in endometrial cancer: Diagnostic and clinical considerations from peritoneal/pelvic washings. Is it still a heated debate?

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