Aquaporin‐2 plays an important role in water transportation through the bladder wall in rats

Morizawa, Yosuke; Torimoto, Kazumasa; Gotoh, Daisuke; Nakai, Yasushi; Miyake, Makito; Hirayama, Akihide; Tanaka, Nobumichi; Fujimoto, Kiyohide

doi:10.1002/nau.23715

Cited by 15 publications

(22 citation statements)

References 25 publications

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“…11 We previously reported that AQP-2 plays an important role in the absorption of saline by the rat bladder. 6 In the present study, we found that the AQP-2 expression level increased only in the saline group, suggesting that sodium may be key to inducing AQP-2 expression in rats. Although the reason is unclear, a similar phenomenon was observed in normal human urothelial cell cultures.…”

Section: T a B L Esupporting

confidence: 46%

“…The change in intravesical fluid solution volume was measured 3 hours later as described in our previous study. 6 The intravesical fluid was immediately shipped on ice to Nagahama Life Oriental Yeast Co, Ltd (Shiga, Japan) where concentrations of sodium and chloride and osmotic pressure were measured using the ion-selective electrode method.…”

Section: Change In Intravesical Fluid In Saline and Glucose Group Ratsmentioning

confidence: 99%

“…We previously demonstrated that the volume of saline decreased in completely filled rat bladders, and aquaporin-2 (AQP-2), which only transports water, played an important role. 6 It was observed that the concentrations of sodium and chlorine also decreased, and the change in concentration diminished when the change in saline volume was diminished after suppression of AQP-2 expression. This suggested that the transport of water was associated with that of sodium through the bladder urothelium.…”

Section: Introductionmentioning

confidence: 98%

“…Therefore, it is important to investigate the absorptive function of the bladder as a daily physiological condition. We previously demonstrated that the volume of saline decreased in completely filled rat bladders, and aquaporin‐2 (AQP‐2), which only transports water, played an important role 6 . It was observed that the concentrations of sodium and chlorine also decreased, and the change in concentration diminished when the change in saline volume was diminished after suppression of AQP‐2 expression.…”

Section: Introductionmentioning

confidence: 99%

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Sodium plays an important role in the absorption of intravesical fluid

Morizawa

Torimoto

Gotoh

et al. 2020

LUTS

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Objectives: To investigate the role of sodium in intravesical absorption of water in the bladder and the sodium pathway in the urothelium. Methods: Adult female Sprague-Dawley rats received either saline or a 5% glucose solution injection into their bladders. The changes in intravesical fluid volume; concentrations of sodium and chlorine and osmolality; and expression of aquaporin-2, epithelial sodium channel, and claudins were compared after 3 hours. Results: Intravesical volume decreased significantly in the saline group compared to that in the 5% glucose solution group. The expression of claudin-3 and-6 was higher in the saline group than in the glucose group. There was a significant correlation between changes in the intravesical saline volume and the concentration of sodium and chlorine. Intravesical administration of amiloride did not affect changes in the fluid volume and concentration of sodium. Conclusions: The presence of sodium is important for the absorption of intravesical fluid through aquaporin-2 in the urinary bladders of rats. Claudin-3 and-6 may be associated with the transport of sodium through the bladder urothelium.

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Section: T a B L Esupporting

confidence: 46%

Section: Change In Intravesical Fluid In Saline and Glucose Group Ratsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Sodium plays an important role in the absorption of intravesical fluid

Morizawa

Torimoto

Gotoh

et al. 2020

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“…There is increasing evidence that the UBM plays an active role in both solute and fluid reabsorption and is able to modify urine composition . While the underlying biochemical mechanisms have yet to be clarified, it is known that the UBM expresses a variety of receptors and channels including several types of aquaporin water channels and the renal epithelial sodium channel (ENaC), both of which play important roles in vasopressin‐mediated water homeostasis in the kidney . An open question, however, is whether the mammalian UBM also expresses vasopressin receptors and whether expression of vasopressin receptors in the UBM is modified by aging.…”

Section: Introductionmentioning

confidence: 99%

Aging increases the expression of vasopressin receptors in both the kidney and urinary bladder

Birder

Jackson

Wein

et al. 2018

Neurourology and Urodynamics

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Aims: The goal of this study was to determine whether aging effects the expression of V 1a and V 2 vasopressin receptors in the urinary bladder mucosa (UBM) and kidney. Methods: UBM and kidneys were obtained from young (3 months-of-age) and old (25-30 months-of-age) female Fisher 344 rats. Tissue samples were analyzed by western blotting for V 1a and V 2 receptor expression, and rat plasma levels of vasopressin levels were measured by ELISA. Results: V 1a and V 2 receptors were detected in both the UBM and kidneys. Aging significantly (P < 0.05) increased the expression of V 2 receptors by 2.80 ± 0.52 and Neurourology and Urodynamics. 2019;38:393-397.wileyonlinelibrary.com/journal/nau

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ICI‐RS 2019 nocturia think tank: How can experimental science guide us in understanding the pathophysiology of nocturia?

Vahabi

Jabr

Fry

et al. 2020

Neurourology and Urodynamics

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Introduction The following is a report on the proceedings of the 2019 International Consultation on Incontinence‐Research Society nocturia think tank (NTT). Objectives The objectives of the 2019 NTT were as follows: (a) to evaluate the role of urothelium in the pathophysiology of nocturia; (b) to determine whether nocturia is a circadian disorder; (c) to discuss the role of melatonin in nocturia; (d) to consider ambulatory urodynamic monitoring in evaluating patients with nocturia; (e) to explore studies of water handling in human compartments utilizing heavy water; and (f) to explore whether basic science is the key to understanding the treatment options for diminished bladder capacity in patients with nocturia. Methods A compendium of discussions of the role of experimental science in understanding the pathophysiology of nocturia is described herein. Results and Conclusions Translational science will play an increasing role in understanding the pathophysiology of nocturia, which may result in improved treatment strategies.

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Aquaporin‐2 plays an important role in water transportation through the bladder wall in rats

Cited by 15 publications

References 25 publications

Sodium plays an important role in the absorption of intravesical fluid

Sodium plays an important role in the absorption of intravesical fluid

Aging increases the expression of vasopressin receptors in both the kidney and urinary bladder

ICI‐RS 2019 nocturia think tank: How can experimental science guide us in understanding the pathophysiology of nocturia?

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