Aquaporin 5 in the eye: Expression, function, and roles in ocular diseases

Zhang, Kaier; Di, Guohu; Bai, Ying; Liu, A.; Bian, Wei; Chen, Peng

doi:10.1016/j.exer.2023.109557

Cited by 1 publication

(2 citation statements)

References 120 publications

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“… 12 , 13 In the case of the LG, the expression of AQP1, 2, 3, 4, 5, 7, 8, 9, and 11 have been confirmed in different species so far, but their exact roles have yet to be clarified. 14 – 16 …”

Section: Discussionmentioning

confidence: 99%

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The Role of Aquaporin 4 in Lacrimal Gland Ductal Fluid Secretion in Mice

Elekes,

Csapó,

Szarka

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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Purpose Earlier reports highlighted the predominant presence of aquaporin 4 (AQP4) in the duct cells of rabbit lacrimal glands (LGs). Whereas significant alterations in AQP4 mRNA levels have been observed in experimental dry eye and during pregnancy, the impact of AQP4 in LG ductal fluid production remains unclear. In our recent work, the role of AQP4 in LG ductal fluid secretion was investigated utilizing wild type (WT) and AQP4 knock out (KO) mice. Methods Tear production was assessed in both WT and KO animals. Immunostaining was used to identify AQP4 protein. Duct segments were harvested from LGs of WT and KO mice. Fluid secretion and filtration permeability (P f ) were quantified using video-microscopy. Ductal tear production, elicited by a cell-permeable cAMP analogue (8-bromo cAMP), carbachol, vasoactive intestinal peptide (VIP), and phenylephrine (PHE), were assessed in both WT and KO ducts. Results A higher expression of AQP4 protein was noted in the duct cells from WT mice when compared to acinar cells. P f did not show notable alterations between WT and AQP4 KO ducts. Carbachol elicited comparable secretory responses in ducts from both WT and KO animals. However, 8-bromo cAMP, VIP, and PHE stimulation resulted in decreased secretion in ducts from AQP4 KO LGs. Conclusions Our findings underscore the functional relevance of AQP4 in the fluid production of mouse LG ducts. AQP4 seems to play different roles in fluid secretions elicited by different secretagogues. Specifically, cAMP-mediated, and adrenergic agonist-related secretions were reduced in AQP4 KO ducts.

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Section: Discussionmentioning

confidence: 99%

“…In gene expression studies and immunofluorescence experiments, the predominant presence of AQP4 is found in the ducts, whereas AQP5 was abundant in the acinar cells both in the rabbit and mouse. 16 – 19 …”

mentioning

confidence: 99%