Aquaporin 5 promotes the proliferation and migration of human gastric carcinoma cells

Huang, Yonghong; Zhou, Xiaoyan; Wang, Hongmei; Xu, Hong; Chen, Jiang; Lv, Nonghua

doi:10.1007/s13277-013-0712-4

Cited by 63 publications

(50 citation statements)

References 26 publications

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“…Recently, Lee et al (11) reported that AQP5 overexpression is significantly associated with lymph node involvement and a poorer prognosis in patients with breast cancer, suggesting the value of AQP5 as a prognostic marker in breast cancer. Huang et al (18) also verified that AQP5 promotes the proliferation and migration of human gastric carcinoma cells. Wang et al (19) performed a study to show that AQP5 was mainly expressed in colorectal carcinoma cells and barely expressed in paraneoplastic normal tissues.…”

Section: Discussionmentioning

confidence: 91%

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

Shan

Cui

et al. 2014

Oncology Reports

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Abstract. The aquaporins (AQPs) are water channel proteins that exhibit several properties related to tumor development. However, the expression and clinical significance of AQP5 in colorectal cancer, particularly the correlation with circulating tumor cells (CTCs), has not been elucidated. The aim of the present study was to determine whether or not the expression of AQP5 is a strong prognostic biomarker for colorectal cancer. The results showed that of 45 tumor specimens, 14 (31.1%) had high levels of expression of AQP5, 29 (64.4%) exhibited a moderate (intermediate) level of staining, and 2 (4.4%) had an absence of AQP5 staining. AQP5 was only occasionally detected in para-neoplastic [3/45 (6.67%)] and normal tissues [3/45 (6.67%)]. AQP5 protein overexpression frequently accompanied gene amplification detection with fluorescence in situ hybridization (FISH). Moreover, AQP5 expression in colorectal cancer cells was upregulated compared to normal colon cells. AQP5 overexpression was also associated with TNM stage (P=0.002), lymph node metastasis (P=0.016), and distant metastasis (P=0.000). The relationships between age, gender, histologic grade and tumor size with expression of AQP5 were not significant (P>0.05). A positive correlation between the number of CTCs and AQP5 expression (P<0.05) was demonstrated. In addition, patients who did not express AQP5 had a superior cumulative survival rate compared to patients with AQP5 positivity. AQP5 may be used as a novel biomarker for colorectal cancer aggressiveness and metastasis, but it does not reflect drug resistance.

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Section: Discussionmentioning

confidence: 91%

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

Shan

Cui

et al. 2014

Oncology Reports

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“…Previous studies indicated that AQP5 protein localized on the membrane of different kinds of cells such as corneal epithelium cells, acinar cells in the lacrimal gland and pulmonary epithelial cells [14]. Overexpression of AQP5 have been reported to be associated with many kinds of cancers such as colorectal, cervical, lung, breast and epithelial ovarian cancer, and to be a potential prognostic biomarkers [15-19]. The role of AQP5 in carcinogenesis is not yet fully understood.…”

Section: Introductionmentioning

confidence: 99%

Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells

et al. 2014

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BackgroundRecent studies suggested that aquaporins 5 (AQP5) was associated with many kinds of cancers and regulated many processes of various kinds of cancer cells. Our previous studies also demonstrated that AQP5 was highly expressed in epithelial ovarian cancer and contributed to the progress of ovarian cancer.MethodsLentivirus for knocking-down the expression of AQP5 was prepared and verified by qPCR and Western blotting. Cell counting kit-8 (CCK8) assay and transwell assay were performed to investigate the role of AQP5 on proliferation and migration of 3AO cells. The effects of down-regulating AQP5 on tumorigenesis were tested by tumor xenografts experiments.ResultsAn effective lentivirus silencing AQP5 expression was obtained and used in this study. Down-regulating AQP5 inhibited proliferation and migration of cultured human epithelial ovarian cancer 3AO Cell. Furthermore, interfering of AQP5 during tumorigenesis could efficiently decrease the tumor growth in athymic mice.ConclusionsThese findings altogether suggest that AQP5 regulated multi processes in ovarian carcinogenesis and may be an attractive therapeutic target.

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“…Previous studies indicated that AQP5 protein localized on the membrane of different kinds of cells such as corneal epithelium cells, acinar cells in the lacrimal gland and pulmonary epithelial cells [14]. Overexpression of AQP5 have been reported to be associated with many kinds of cancers such as colorectal, cervical, lung, breast and epithelial ovarian cancer, and to be a potential prognostic biomarkers [15][16][17][18][19]. The role of AQP5 in carcinogenesis is not yet fully understood.…”

Section: Introductionmentioning

confidence: 99%

Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells

Cao

Zhu

Zhang

et al. 2014

Journal of Ovarian Research

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Background: Recent studies suggested that aquaporins 5 (AQP5) was associated with many kinds of cancers and regulated many processes of various kinds of cancer cells. Our previous studies also demonstrated that AQP5 was highly expressed in epithelial ovarian cancer and contributed to the progress of ovarian cancer. Methods: Lentivirus for knocking-down the expression of AQP5 was prepared and verified by qPCR and Western blotting. Cell counting kit-8 (CCK8) assay and transwell assay were performed to investigate the role of AQP5 on proliferation and migration of 3AO cells. The effects of down-regulating AQP5 on tumorigenesis were tested by tumor xenografts experiments.

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Aquaporin 5 promotes the proliferation and migration of human gastric carcinoma cells

Cited by 63 publications

References 26 publications

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

AQP5: A novel biomarker that predicts poor clinical outcome in colorectal cancer

Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells

Down-regulated aquaporin 5 inhibits proliferation and migration of human epithelial ovarian cancer 3AO cells

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