Aquaporin in different moult stages of a freshwater decapod crustacean: Expression and participation in muscle hydration control

Foguesatto, Kamila; Boyle, Robert T.; Rovani, Monique Tomazele; Freire, Carolina A.; Souza, Maurício de

doi:10.1016/j.cbpa.2017.03.003

Cited by 26 publications

(22 citation statements)

References 39 publications

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“…Previous gene expression studies in Palaemon argentinus [12] and S. paramamosain [2] have explored the role of aquaporin with Na+/K+-ATPase and Na+/K+/2Cl− cotransporter in the uptake of water during the molting processes; these are osmoregulation-related genes with the same trends of expression levels in each of the molting stages in our study. We inferred that aquaporin and other osmoregulation-related genes act synergistically to regulate osmotic pressure and water absorption after molting.…”

Section: Expression Patterns Of Degs Associated With Cuticle and Cytomentioning

confidence: 82%

“…Previous studies have made signi cant advances in our understanding of the molting cycle concerning growth characteristics, material changes, energy metabolism, and hormone regulation at the ecological and physiological levels [5][6][7][8][9]. Moreover, recent studies have explored molting-related genes and their effects on protein synthesis [10], chitin metabolism [11], and muscle hydration control [12] during the molting cycle. However, the molecular events and mechanisms associated with the dynamic process of molting remain poorly understood in crustaceans, especially in crabs.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain

Liu

Fu³

et al. 2021

Preprint

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Background: Molting is an inherent cyclic process that occurs in all crustaceans and is essential for growth, metamorphosis, and reproduction. Recent studies have shown that many organs and systems are involved in molting regulation, including the hepatopancreas, the immune system, and production of ecdysteroids by Y-organs. However, the molecular mechanisms underlying the dynamic process of crab molting remain poorly understood at the whole-individual level. Therefore, we investigated global expression changes in the transcriptomes of the mud crab Scylla paramamosain, the most cultured crab species worldwide. We revealed a cascade of sequential expression events of molting-related genes involved in various aspects of the molting process by using whole-body sequencing of juvenile crabs. Results: RNA-sequencing (RNA-seq) produced 139.49 Gb of clean reads and 20,436 differentially expressed genes (DEGs) among different molting stages. The expression patterns for genes involved in several molecular events critical for molting, such as cuticle reconstruction, cytoskeletal structure remodeling, hormone regulation, immune responses, and metabolism, were characterized and considered as mechanisms underlying molting in S. paramamosain. Among these genes, we identified 10,695 DEGs in adjacent molting stages. In further GO and KEGG analyses, the significantly enriched pathways included structural constituents of cuticle, binding and chitin metabolic processes, steroid hormone biosynthesis, insulin resistance, and amino sugar metabolic processes. The expression profiles of 12 functional genes detected via RNA-Seq were corroborated via real-time RT-PCR assays. The results indicated gene expression profiles across the molting cycle and identified possible activation pathways for future investigation of the underlying molecular mechanisms. Conclusions: This study establishes a comprehensive transcriptomic repertoire for the molting process in S. paramamosain. The data generated in this study will serve as an important transcriptomic resource for the crab research community to facilitate gene and genome annotation and provide fundamental support for future research on molecular mechanisms in this species. Furthermore, the comprehensive analysis results will improve our understanding of morphological variation in the molting cycle and serve as a potential blueprint for future research on molting in crustaceans and other animals.

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Section: Expression Patterns Of Degs Associated With Cuticle and Cytomentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain

Liu

Fu³

et al. 2021

Preprint

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“…The gill Lv-AE3 mRNA increased to a very high level during early postmolting (P1, Figure 5B ), the stage in which shrimp absorb a large amount of water, indicating that Lv -AE3 may mediate active ion exchange at this period to balance internal salinity and acidity. In the freshwater palaemonid shrimp ( Palaemonetes argentinus ), the haemolymph osmolality reduced after ecdysis while the aquaporin mRNA kept equally ( Foguesatto et al, 2017 ), indicating that crustaceans may absorb water for expanding the new exoskeleton by participation of the ion channels.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Anion Exchange Protein 3 in Pacific White Shrimp (Litopenaeus vannamei): mRNA Profiles for Tissues, Ontogeny, Molting, and Ovarian Development and Its Potential Role in Stress-Induced Gill Damage

Zhang

Yang

et al. 2021

Front. Physiol.

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Bicarbonate (HCO3–) transport mechanisms play an essential role in the acid-base homeostasis of aquatic animals, and anion exchange protein 3 (AE3) is a membrane transport protein that exchanges Cl–/HCO3– across the cell membrane to regulate the intracellular pH. In this study, the full-length cDNA of AE3 (Lv-AE3) was obtained from the Pacific white shrimp (Litopenaeus vannamei). The Lv-AE3 cDNA is 4,943 bp in length, contains an open reading frame of 2,850 bp, coding for a protein of 949 amino acids with 12 transmembrane domains. Lv-AE3 shows high sequence homology with other AE3 at the protein level. Lv-AE3 mRNA was ubiquitously detected in all tissues selected, with the highest expression level in the gill, followed by the ovary, eyestalk and brain. By in situ hybridization, Lv-AE3-positive cells were shown predominant localization in the secondary gill filaments. The expression levels of Lv-AE3 were further investigated during the essential life processes of shrimp, including ontogeny, molting, and ovarian development. In this case, the spatiotemporal expression profiles of Lv-AE3 in L. vannamei were highly correlated with the activities of water and ion absorption; for example, increased mRNA levels were present after hatching, during embryonic development, after ecdysis during the molt cycle, and in the stage IV ovary during gonadal development. After low/high pH and low/high salinity challenges, the transcript levels of Lv-AE3 were reduced in the gill, while the cell apoptosis rate increased. In addition, knockdown of Lv-AE3 mRNA expression induced cell apoptosis in the gill, indicating a potential link between Lv-AE3 and gill damage. Altogether, this study thoroughly investigated the relationship between the mRNA expression profiles of Lv-AE3 and multiple developmental and physiological processes in L. vannamei, and it may benefit the protection of crustaceans from fluctuated aquatic environments.

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“…The description of setogenesis has been used widely in a variety of studies on crustaceans (Sousa and Petriella, 2006;Sugumar et al, 2013;Foguesatto et al, 2017). However, the lack of images, detailed descriptions, and differences in the nomenclature of the observed structures may create confusion.…”

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confidence: 99%

“…Palaemon argentinus (Nobili, 1901), a freshwater shrimp that reaches a maximum size of 32 mm (females) and 29 mm (males) (Bond-Buckup and Buckup, 1989) can be maintained in the laboratory and offers a great variety of study opportunities, such as: reproductive biology (Schuldt and Capítulo, 1985), osmoregulation (Lignot et al, 1999;Ituarte et al, 2016), morphology, histology of the digestive tract (Sousa and Petriella, 2006), growth (Montagna, 2011), parasitism (Neves et al, 2004), moult cycle in the natural environment (Díaz et al, 1998;Felix and Petriella, 2003), behavioral ecology (Gancedo and Ituarte, 2017), and recently also the regulation of cell volume during the moult cycle (Foguesatto et al, 2017). Setogenesis in P. argentinus has been described only for juveniles (Díaz et al, 1998); some substages of the moult cycle of this species, however, have not yet been elucidated.…”

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confidence: 99%

Setogenesis and characterization of the new moult substages in the freshwater shrimp Palaemon argentinus (Nobili, 1901) (Caridea: Palaemonidae)

2019

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The moult cycle of crustaceans is continuous and during different stages of this cycle, physiological, biochemical and morphological changes occur. Therefore, understanding the different stages of the moult cycle in the target species becomes critical for a wide range of biological studies. Here we describe the natural cycle changes in the freshwater shrimp Palaemon argentinus and identify two new substages of post-moult, B 1 and B 2 , that are substages occurring before the intermoult, a stage widely used in crustacean studies. Furthermore, we present a more detailed description of stages already known, describing modifications of the structures and its presence or absence in each stage, in conjunction with explanatory pictures. We also indicate the duration for each stage of the cycle, thus expanding our knowledge of the moult cycle and setogenesis for P. argentinus.

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Aquaporin in different moult stages of a freshwater decapod crustacean: Expression and participation in muscle hydration control

Cited by 26 publications

References 39 publications

Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain

Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain

Molecular Identification of Anion Exchange Protein 3 in Pacific White Shrimp (Litopenaeus vannamei): mRNA Profiles for Tissues, Ontogeny, Molting, and Ovarian Development and Its Potential Role in Stress-Induced Gill Damage

Setogenesis and characterization of the new moult substages in the freshwater shrimp Palaemon argentinus (Nobili, 1901) (Caridea: Palaemonidae)

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