Aquaporin Isoforms Responsive to Salt and Water Stresses and Phytohormones in Radish Seedlings

Suga, Shinobu; Komatsu, Setsuko; Maeshima, Masayoshi

doi:10.1093/pcp/pcf148

Cited by 148 publications

(109 citation statements)

References 35 publications

(47 reference statements)

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“…OsPIP1;2, OsPIP1;3, OsPIP2;1, OsPIP2;3, OsPIP2;4, OsPIP2;5 and OsPIP2;6, were transcribed at higher rates in roots than leaves and only one PIP gene, OsPIP2;7, followed the pattern of mRNA accumulation of higher levels in leaves than roots. Inconsistency between aquaporin expression at the mRNA level and protein accumulation was also noted in a previous study [8,30]. These authors postulated that post-transcriptional mechanisms resulted in this, e.g.…”

Section: Pip Expression In Upland Rice and Lowland Rice Under Normal supporting

confidence: 63%

“…Different PIP genes exhibit different expression patterns. Water deficit was shown to enhance the expression of rice OsPIP1a and OsPIP2a [5], tomato TRAMP [6], Nicotiana excelsior NeMip2 and NeMip3 [7] and radish RsPIP2 [8], but reduced Nicotiana glauca NgMIP4 [9] and rice RWC1 [10]. In a recent study, both up-and down-regulation of PIP expression were observed under water deficit induced by mannitol in Arabidopsis [11].…”

Section: Introductionmentioning

confidence: 99%

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Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins (PIPs), was studied at both the protein and mRNA level in upland rice (Oryza sativa L. cv. Zhonghan 3) and lowland rice (Oryza sativa L. cv. Xiushui 63) when they were water stressed by treatment with 20% polyethylene glycol (PEG). Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1;2, Os-PIP1;3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1;2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid (ABA) level in upland rice but not in lowland rice under water deficit. Treatment with 60 µM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling pathways during water deficit.

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Section: Pip Expression In Upland Rice and Lowland Rice Under Normal supporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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“…Gibberellic acid and abscisic acid suppressed the levels of mRNAs of RsPIP2-1, RsPIP2-2 and RsPIP2-3 and the protein level of RsPIP2-1 in roots. On the other hand, the protein levels of RsPIP1-group members and RsTIPs were scarcely changed by these phytohormones [104].…”

Section: Relationships Between Plant Aquaporins and Hormonesmentioning

confidence: 94%

“…Brassinolide may control aquaporin activities in Arabidopsis thaliana, which has been shown to be involved in the modification of the water-transport properties of cell membranes [105], however, mRNA and protein levels of aquaporin isoforms in root and shoot of radish were not affected by brassinolide treatment [104]. Ethylene significantly increased stomatal conductance, root hydraulic conductivity (L p ), and root oxygen uptake in hypoxic seedlings.…”

Section: Relationships Between Plant Aquaporins and Hormonesmentioning

confidence: 98%

Aquaporin structure–function relationships: Water flow through plant living cells

Zhao

Shao

Chu

2008

Colloids and Surfaces B: Biointerfaces

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Plant aquaporins play an important role in water uptake and movement-an aquaporin that opens and closes a gate that regulates water movement in and out of cells. Some plant aquaporins also play an important role in response to water stress. Since their discovery, advancing knowledge of their structures and properties led to an understanding of the basic features of the water transport mechanism and increased illumination to water relations. Meanwhile, molecular and functional characterization of aquaporins has revealed the significance of their regulation in response to the adverse environments such as salinity and drought. This paper reviews the structure, species diversity, physiology function, regulation of plant aquaporins, and the relations between environmental factors and plant aquaporins. Complete understanding of aquaporin function and regulation is to integrate those mechanisms in time and space and to well regulate the permeation of water across biological membranes under changing environmental and developmental conditions.

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“…Nutritional deficiency in general decreases the expression of Lp and aquaporin genes in roots [4], however, specific genes were induced under special conditions, such as Arabidopsis NIP5;1 by boron-deficiency [24] and Arabidopsis TIP2;1 by nitrogen-deficiency [17]. Low temperature [13], phytohormones [23], and far-red light [22] also regulated the expression of plant aquaporins. These observations indicate that adequate regulation of water homeostasis via aquaporins is common in plant cells adjusting to various environments.…”

Section: Water Transport Of Barley Pipsmentioning

confidence: 99%

Barley plasma membrane intrinsic proteins (PIP Aquaporins) as water and CO2 transporters

Katsuhara

Hanba

2008

Pflugers Arch - Eur J Physiol

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We identified barley aquaporins, and demonstrated that one, HvPIP2;1, transports water and CO 2 . Regarding water homeostasis in plants, regulations of aquaporin expression were observed in many plants under several environmental stresses. Under salt stress, a number of plasma-membrane type aquaporins were down-regulated, which can prevent continuous dehydration resulting in cell death. The leaves of transgenic rice plants that expressed the largest amount of HvPIP2;1 showed a 40% increase in internal CO 2 conductance compared with leaves of wild-type rice plants. The rate of CO 2 assimilation also increased in the transgenic plants. The goal of our plant aquaporin research is to determine the key aquaporin species responsible for water and CO 2 transport, and to improve plant water relations, stress tolerance, CO 2 uptake/assimilation, and plant productivity via molecular breeding of aquaporins.

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Aquaporin Isoforms Responsive to Salt and Water Stresses and Phytohormones in Radish Seedlings

Cited by 148 publications

References 35 publications

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

Aquaporin structure–function relationships: Water flow through plant living cells

Barley plasma membrane intrinsic proteins (PIP Aquaporins) as water and CO2 transporters

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