Aquaporin-mediated dysregulation of cell migration in disease states

Smith, Ian M.; Banerjee, Shohini; Moses, Allison K.; Stroka, Kimberly M.

doi:10.1007/s00018-022-04665-9

Cited by 8 publications

(5 citation statements)

References 182 publications

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“…Previous studies showed that cell migration can be reduced by silencing expression of AQP3 (De Ieso & Yool, 2018; Pimpao et al., 2020; Rodrigues et al., 2019; Silva et al., 2022), evidencing that AQP3 has a pivotal role in cancer metastasis. Moreover, overexpression of AQP3 in cancer cells was shown to stimulate several intracellular signalling pathways, promoting cell proliferation, migration and invasion as well as aggravation of epithelial‐to‐mesenchymal transition (Smith et al., 2023). The role of AQP3 in signalling mechanisms in which H 2 O 2 acts as an important second messenger may explain, at least in part, the effect of gold compounds on cell migration of both MNT‐1 and A375 cells.…”

Section: Discussionmentioning

confidence: 99%

Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration

da Silva,

Pimpão,

Paccetti‐Alves

et al. 2024

The Journal of Physiology

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Aquaporin‐3 (AQP3) is a membrane channel with dual aquaglyceroporin/peroxiporin activity, facilitating the diffusion of water, glycerol and H2O2 across cell membranes. AQP3 shows aberrant expression in melanoma and its role in cell adhesion, migration and proliferation is well described. Gold compounds were shown to modulate AQP3 activity with reduced associated toxicity, making them promising molecules for cancer therapy. In this study, we validated the phenotype resulting from AQP3‐silencing of two melanoma cell lines, MNT‐1 and A375, which resulted in decreased H2O2 permeability. Subsequently, the AQP3 inhibitory effect of a new series of organogold compounds derived from Auphen, a potent AQP3 inhibitor, was first evaluated in red blood cells (RBCs) that highly express AQP3, and then in HEK‐293T cells with AQP3 overexpression to ascertain the compounds’ specificity. The first screening in RBCs unveiled two organogold compounds as promising blockers of AQP3 permeability. Moderate reduction of glycerol permeability but drastic inhibition of H2O2 permeability was detected for some of the gold derivatives in both AQP3‐overexpressing cells and human melanoma cell lines. Additionally, all compounds were effective in impairing cell adhesion, proliferation and migration, although in a cell type‐dependent manner. In conclusion, our data show that AQP3 peroxiporin activity is crucial for melanoma progression and highlight organogold compounds as promising AQP3 inhibitors with implications in melanoma cell adhesion, proliferation and migration, unveiling their potential as anticancer drugs against AQP3‐overexpressing tumours. imageKey points AQP3 affects cellular redox balance. Gold compounds inhibit AQP3 permeability in melanoma cells. AQP3 is involved in cell adhesion, proliferation and migration of melanoma. Blockage of AQP3 peroxiporin activity impairs melanoma cell migration. Gold compounds are potential anticancer drug leads for AQP3‐overexpressing cancers.

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Section: Discussionmentioning

confidence: 99%

Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration

da Silva,

Pimpão,

Paccetti‐Alves

et al. 2024

The Journal of Physiology

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“…18 The EGF, which is abundantly present in colostrum, is a known upstream regulator of AQP-3 expression in cancer cell cultures. 34,35 Additionally, lactoferrin, exopolysaccharides, and bioactive peptides with low molecular weight are present in fermented milk and serum, which can stimulate the proliferation and healing of skin cells.…”

Section: Investigated the Effects Of Bovine Colostrum Onmentioning

confidence: 99%

Exploring the potential of bovine colostrum as a bioactive agent in human tissue regeneration: A comprehensive analysis of mechanisms of action and challenges to be overcome

dos Santos,

Kraus,

da Silva Nascente

2024

Cell Biochemistry & Function

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The study examines bovine colostrum as a potent source of bioactive compounds, particularly growth factors, for tissue regeneration in humans. While previous research has hinted at therapeutic benefits, a comprehensive understanding of its mechanisms remains elusive, necessitating further investigation. This review analyzes nine selected scientific articles on bovine colostrum's bioactive potential in tissue regeneration. In vitro studies highlight its positive impact on cell behavior, including reduced proliferation and induced differentiation. Notably, optimal concentrations and specific colostrum components, such as extracellular vesicles and insoluble milk fat, show more favorable outcomes. In vivo studies underscore bovine colostrum as a promising natural resource for wound healing, despite some studies failing to identify associated benefits. Further research is crucial to unravel the intricate mechanisms, grasp the full potential in regenerative medicine, and develop more effective wound healing therapies. This refined understanding will pave the way for harnessing the complete regenerative potential of bovine colostrum in clinical applications.

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“…The EGF, which is abundantly present in colostrum, is a known upstream regulator of AQP-3 expression in cancer cell cultures (LI et al, 2013;SMITH et al, 2023). Additionally, lactoferrin, exopolysaccharides, and bioactive peptides with low molecular weight are present in fermented milk and serum, which can stimulate the proliferation and healing of skin cells.…”

Section: Keratinocytesmentioning

confidence: 99%

Exploring the Potential of Bovine Colostrum as a Bioactive Agent in Human Tissue Regeneration: A Comprehensive Analysis of Mechanisms of Action and Challenges to be Overcome

Santos,

Kraus,

Nascente

2024

Preprint

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Bovine colostrum is a rich source of bioactive compounds, including growth factors, that may promote tissue regeneration. Studies suggest it has potential therapeutic benefits in humans, but further research is needed to fully understand its mechanisms of action. This study aims to review scientific articles on the use of bovine colostrum as a bioactive agent for tissue regeneration in humans and twelve articles were selected. In vitro studies show that bovine colostrum can benefit cells by reducing cell proliferation and inducing differentiation. The concentration of colostrum used has a direct impact on the results, with no positive results observed at some high concentrations (20%). Extracted portions of colostrum (EVs and IMF) showed better results than the complete colostrum. In vivo studies show that bovine colostrum is a promising natural source for wound healing, as its bioactive factors help in the orderly progression of the healing process. However, some studies did not find any benefit related to tissue regeneration, and more research is needed to explore the full potential of colostrum in regenerative medicine and to develop more effective therapies for wound healing.

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Aquaporin-mediated dysregulation of cell migration in disease states

Cited by 8 publications

References 182 publications

Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration

Blockage of aquaporin‐3 peroxiporin activity by organogold compounds affects melanoma cell adhesion, proliferation and migration

Exploring the potential of bovine colostrum as a bioactive agent in human tissue regeneration: A comprehensive analysis of mechanisms of action and challenges to be overcome

Exploring the Potential of Bovine Colostrum as a Bioactive Agent in Human Tissue Regeneration: A Comprehensive Analysis of Mechanisms of Action and Challenges to be Overcome

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