Aquaporins in lacrimal glands and their role in dry eye disease

Bai, Ying; Zhang, Kaier; Cao, Xin; Chen, Peng

doi:10.1016/j.exer.2023.109676

Cited by 5 publications

(2 citation statements)

References 95 publications

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“…These findings suggest that AQP channels are suggested to have a significant impact on the pathophysiological processes of LG function. 36 Further clarification of the role of AQP5 holds promise for the development of specific therapeutic interventions, such as gene transfer, aimed at restoring the function of the channel. These interventions could potentially restore lacrimal gland function in cases of dry eye disease.…”

Section: Discussionmentioning

confidence: 99%

The Role of Aquaporin 4 in Lacrimal Gland Ductal Fluid Secretion in Mice

Elekes,

Csapó,

Szarka

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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Purpose Earlier reports highlighted the predominant presence of aquaporin 4 (AQP4) in the duct cells of rabbit lacrimal glands (LGs). Whereas significant alterations in AQP4 mRNA levels have been observed in experimental dry eye and during pregnancy, the impact of AQP4 in LG ductal fluid production remains unclear. In our recent work, the role of AQP4 in LG ductal fluid secretion was investigated utilizing wild type (WT) and AQP4 knock out (KO) mice. Methods Tear production was assessed in both WT and KO animals. Immunostaining was used to identify AQP4 protein. Duct segments were harvested from LGs of WT and KO mice. Fluid secretion and filtration permeability (P f ) were quantified using video-microscopy. Ductal tear production, elicited by a cell-permeable cAMP analogue (8-bromo cAMP), carbachol, vasoactive intestinal peptide (VIP), and phenylephrine (PHE), were assessed in both WT and KO ducts. Results A higher expression of AQP4 protein was noted in the duct cells from WT mice when compared to acinar cells. P f did not show notable alterations between WT and AQP4 KO ducts. Carbachol elicited comparable secretory responses in ducts from both WT and KO animals. However, 8-bromo cAMP, VIP, and PHE stimulation resulted in decreased secretion in ducts from AQP4 KO LGs. Conclusions Our findings underscore the functional relevance of AQP4 in the fluid production of mouse LG ducts. AQP4 seems to play different roles in fluid secretions elicited by different secretagogues. Specifically, cAMP-mediated, and adrenergic agonist-related secretions were reduced in AQP4 KO ducts.

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Section: Discussionmentioning

confidence: 99%

The Role of Aquaporin 4 in Lacrimal Gland Ductal Fluid Secretion in Mice

Elekes,

Csapó,

Szarka

et al. 2024

Invest. Ophthalmol. Vis. Sci.

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“…Several studies have found that CDC family genes are associated with apoptosis 17 , which induces cellular senescence owing to its apoptotic mechanism, with the induction of cell senescence possibly resulting in numerous ocular surface disorders, including DED 18 . Simultaneously, cellular senescence, as one of the key causes of DED, is associated with alterations in the structure and function of the lachrymal gland, such as atrophy and reduced protein secretion 19 , with a higher probability of lacrimal gland lesions in older females 20 . The MCM gene family is widely found in eukaryotes such as humans, plays a role in regulating cell division, and is a key factor in initiating DNA replication 21 .…”

Section: Discussionmentioning

confidence: 99%

The shared mechanism and potential diagnostic markers for premature ovarian failure and dry eye disease

Long,

Wu,

Jiang

et al. 2024

Sci Rep

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Premature ovarian failure (POF), which is often comorbid with dry eye disease (DED) is a key issue affecting female health. Here, we explored the mechanism underlying comorbid POF and DED to further elucidate disease mechanisms and improve treatment. Datasets related to POF (GSE39501) and DED (GSE44101) were identified from the Gene Expression Omnibus (GEO) database and subjected to weighted gene coexpression network (WGCNA) and differentially expressed genes (DEGs) analyses, respectively, with the intersection used to obtain 158 genes comorbid in POF and DED. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses of comorbid genes revealed that identified genes were primarily related to DNA replication and Cell cycle, respectively. Protein–Protein interaction (PPI) network analysis of comorbid genes obtained the 15 hub genes: CDC20, BIRC5, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, and CDCA3. To validate the analysis results, additional POF- and DED-related datasets (GSE48873 and GSE171043, respectively) were selected. miRNAs-lncRNAs-genes network and machine learning methods were used to further analysis comorbid genes. The DGIdb database identified valdecoxib, amorfrutin A, and kaempferitrin as potential drugs. Herein, the comorbid genes of POF and DED were identified from a bioinformatics perspective, providing a new strategy to explore the comorbidity mechanism, opening up a new direction for the diagnosis and treatment of comorbid POF and DED.

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