Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates

Cheung, Cecilia Y.; Anderson, Debra F.; Brace, Robert A.

doi:10.14814/phy2.12868

Cited by 9 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with this concept is our recent finding that AQP1 expression in the ovine amnion was positively correlated with the rate of fluid transfer between the amniotic fluid compartment and fetal intramembranous vasculature, thus potentially participate in AFV regulation (Cheung et al. ).…”

Section: Introductionsupporting

confidence: 59%

See 1 more Smart Citation

High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates

et al. 2018

Self Cite

View full text Add to dashboard Cite

Western style, high‐fat diet (HFD) and associated high lipid levels have deleterious effects on fetal and placental development independent of maternal obesity and/or diabetes. Our objectives were to determine whether HFD without development of obesity would alter amniotic fluid volume (AFV) and amnion aquaporin (AQP) expression in a non‐human primate model. Japanese macaques were fed either a control diet or HFD before and during pregnancy. The four quadrant amniotic fluid index (AFI) was used as an ultrasonic estimate of AFV at 120 days gestation. Amnion samples were collected at 130 days gestation by cesarean section and AQP mRNA levels were determined by quantitative RT‐PCR. Similar to that in human, AQP1, AQP3, AQP8, AQP9, and AQP11 were expressed in the macaque amnion with significant differences in levels among AQPs. In macaque, neither individual AQPs nor expression profiles of the five AQPs differed between control and non‐obese HFD animals. There were regional differences in AQP expression in that, AQP1 mRNA levels were highest and AQP8 lowest in reflected amnion while AQP3, AQP9, and AQP11 were not different among amnion regions. When subdivided into control and HFD groups, AQP1 mRNA levels remain highest in the reflected amnion of both groups. The HFD did not significantly affect the AFI, but AFI was positively correlated with AQP11 mRNA levels independent of diet. Collectively, these data suggest that HFD in pregnant non‐obese individuals may have at most modest effects on AFV as the AFI and amnion AQP expression are not substantially altered.

show abstract

Section: Introductionsupporting

confidence: 59%

“…In ovine amnion, AQP8 mRNA level is highest while AQP9 is lowest when compared to AQP1, AQP3 and AQP11 levels (Cheung et al. ). Whether such differences would alter amniotic fluid dynamics is unknown.…”

Section: Discussionmentioning

confidence: 99%

High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The methodologies were similar to those recently described (Cheung et al. ). Single strand cDNA synthesis from total RNA (2 μ g) was carried out using MultiScribe reverse transcriptase and random primers in the presence of RNase inhibitor (Applied Biosystems, Life Technologies, Foster City, CA).…”

Section: Methodsmentioning

confidence: 99%

“…The experimental protocols were described elsewhere (Cheung et al. ). Tissues were collected 2 days after initiating one of four different experimental conditions: control, urine drainage without fluid replacement, urine drainage with isovolumic fluid replacement (lactated Ringer's solution), and continuous intra‐amniotic fluid infusion (2 L/day of lactated Ringer's solution).…”

Section: Methodsmentioning

confidence: 99%

Transport-associated pathway responses in ovine fetal membranes to changes in amniotic fluid dynamics

2017

Self Cite

View full text Add to dashboard Cite

Current evidence suggests that amniotic fluid volume (AFV) is actively regulated by vesicular transport of amniotic fluid outward across the amnion and into the underlying fetal vasculature in the placenta. Our objective was to determine whether gene expression profiles of potential stimulators, inhibitors, and mediators of vesicular transport are altered in response to changes in intramembranous absorption (IMA) rate. Samples of ovine amnion and chorion were obtained from fetal sheep with normal, experimentally reduced or increased AFVs and IMA rates. Amnion and chorion levels of target mRNAs were determined by RT‐qPCR. In the amnion, caveolin‐1 and flotillin‐1 mRNA levels were unchanged during alterations in IMA rate. However, levels of both were significantly higher in amnion than in chorion. Tubulin‐α mRNA levels in the amnion but not in chorion were reduced when IMA rate decreased, and amnion levels correlated positively with IMA rate (P < 0.05). Dynamin‐2 mRNA levels were not altered by experimental conditions. Vascular endothelial growth factor (VEGF 164 and VEGF 164b) mRNA levels increased during both increases and decreases in IMA rate, whereas soluble Flt‐1 levels did not change. Neither HIF‐1α nor PBEF mRNA levels in the amnion were correlated with VEGF 164 expression levels and were not related to IMA rate. Collectively, our findings suggest that changes in amnion microtubule expression may be important in the regulation of transcellular vesicular transport of amniotic fluid and thus modulate IMA rate. Further, our results are consistent with the concept that the amnion is the rate‐limiting layer for amniotic fluid transport.

show abstract

“…In animal studies, the effect of fetal urinary prostaglandin E 2 on IM transport was explored but results did not support such a role (Cheung, Beardall, Anderson, & Brace, ). Conversely, AQP1 appeared to play a positive role in regulating the rate of IM transport (Cheung, Anderson, & Brace, ) while the growth and permeability factor vascular endothelial growth factor (VEGF) may mediate amniotic fluid transport across the amnion (Cheung, Anderson, & Brace, ). Recently, we reported that IM transport across the amnion is a multifactorial regulatory process that involves cellular transport pathways and signaling modulators.…”

Section: Introductionmentioning

confidence: 99%

Altered proteomics profile in the amnion of patients with oligohydramnios

Cheung

Brace

2020

Physiol Rep

Self Cite

View full text Add to dashboard Cite

In pregnancy, idiopathic oligohydramnios is an obstetrical complication that compromises maternal health with poor perinatal outcome. Effective therapeutic treatment of this condition has been hampered by the unknown etiology and lack of understanding of cellular and molecular mechanisms that underlie idiopathic oligohydramnios. Amniotic fluid volume (AFV) is determined by intramembranous (IM) transport of amniotic fluid across the amnion and this pathway is regulated to maintain AFV within the normal range. To gain understanding of the causes of idiopathic oligohydramnios, we performed proteomics analysis of the human amnion to investigate the changes in protein expression profiles of cellular transport pathways and regulators in patients with oligohydramnios. Placental amnions from five patients with normal pregnancies and five patients with oligohydramnios were subjected to proteomics experiments followed by bioinformatics analysis. Using Ingenuity Pathway Analysis (IPA) software, five categories of biological functions and multiple canonical pathways within each category were revealed. The top differentially expressed proteins that participate in mediating these pathways were identified. The functional pathways activated include: (a) cellular assembly and organization, (b) cell signaling and energy metabolism, and (c) immunological, infectious, and inflammatory functions. Furthermore, the analysis identified the category of pathways that facilitate molecular endocytosis and vesicular uptake. Under oligohydramniotic conditions, the mediators of clathrin vesicle‐mediated uptake and transport as well as intracellular trafficking mediators were up‐regulated. These findings suggest that idiopathic oligohydramnios may be associated with alternations in cellular organization and immunological functions as well as increases in activity of vesicular transport pathways across the amnion.

show abstract

Aquaporins in ovine amnion: responses to altered amniotic fluid volumes and intramembranous absorption rates

Cited by 9 publications

References 30 publications

High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates

High-fat diet effects on amniotic fluid volume and amnion aquaporin expression in non-human primates

Transport-associated pathway responses in ovine fetal membranes to changes in amniotic fluid dynamics

Altered proteomics profile in the amnion of patients with oligohydramnios

Contact Info

Product

Resources

About