Aquaporins Involvement in Pancreas Physiology and in Pancreatic Diseases

Arsenijević, Tatjana; Perret, Jason; Laethem, Jean‐Luc Van; Delporte, Christine

doi:10.3390/ijms20205052

Cited by 27 publications

(20 citation statements)

References 125 publications

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“…It is often argued that imaging may not be as effective for diagnosis as biomarkers, which are at the center stage for early detection 66 - 69 . However, imaging is always required for radiation therapy treatment planning.…”

Section: Discussionmentioning

confidence: 99%

Pancreatic Cancer detection via Galectin-1-targeted Thermoacoustic Imaging: validation in an in vivo heterozygosity model

Qin¹,

Qin²,

Yuan³

et al. 2020

Theranostics

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Purpose: To investigate the feasibility of microwave-induced thermoacoustic imaging (MTAI) in detecting small pancreatic tumors (< 10 mm in diameter) and to complement the limitation of current clinical imaging methods. Methods: A home-made MTAI system composed of a portable antenna and pulsed microwave generator was developed. The thermoacoustic nanoparticles were composed of the galectin-1 antibody for targeting pancreatic tumors and Fe 3 O 4 nanoparticles as microwave absorbers (anti-Gal1-Fe 3 O 4 nanoparticles). The microwave absorption properties of the nanoparticles were measured with a vector network analyzer and the resolving power of MTAI was investigated by imaging excised pancreatic tumors of different sizes (diameters of 1.0 mm, 3.1 mm, 5.0 mm, 7.2 mm). To simulate actual imaging scenarios, an in vivo heterozygosity model was constructed by covering the pancreatic tumors (~ 3 mm in diameter) in BALB/c nude mice with biologic tissue (~ 5 cm in depth). MTAI images of the heterozygosity model were acquired with/without the injection of the anti-Gal1-Fe 3 O 4 nanoparticles and the thermoacoustic contrast from pancreatic tumors was evaluated with Student's paired t test. The data were analyzed with analysis of variance and nonparametric statistics. Results : Following intravenous infusion, anti-Gal1-Fe 3 O 4 nanoparticles efficiently accumulated in the tumor. The MTAI contrast enhancement in pancreatic tumors with anti-Gal1-Fe 3 O 4 nanoparticles was verified in vitro and in vivo . The pancreatic tumors were visible in nude mice examined with MTAI with a mean contrast enhancement ratio of 2.3 ± 0.15 (standard error of the mean) ( P =. 001) at 6 h post-injection of the nanoparticles. MTAI identified tiny pancreatic tumors in deep tissues with high fidelity. Conclusion : MTAI offers deep imaging depth and high contrast when used with anti-Gal1-Fe 3 O 4 nanoparticles. It can identify pancreatic tumors smaller than 5 mm, which is beyond the identification limit size (~10 mm) of other nondestructive clinical imaging methods. Thus, MTAI has great potential as an alternative imaging modality for early pancreatic cancer detection.

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Section: Discussionmentioning

confidence: 99%

Pancreatic Cancer detection via Galectin-1-targeted Thermoacoustic Imaging: validation in an in vivo heterozygosity model

Qin¹,

Qin²,

Yuan³

et al. 2020

Theranostics

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“…Aquaporins (AQPs) are activated by Ca 2+ and mediate a water flow through the luminal membrane. The role of some AQP types in physiological and pathophysiological processes of exocrine pancreas has already been reviewed (Burghardt et al, 2006;Delporte, 2014;Arsenijevic et al, 2019). AQP1 is expressed at the apical and basolateral membrane of centro-acinar cells and intercalated ductal cells (Burghardt et al, 2003;Burghardt et al, 2006) and is also expressed in capillary endothelial cells and at the pancreatic zymogen granule membrane (Cho et al, 2002;Burghardt et al, 2003).…”

Section: Aquaporin Channelsmentioning

confidence: 99%

Ion Channel Signature in Healthy Pancreas and Pancreatic Ductal Adenocarcinoma

et al. 2020

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Pancreatic ductal adenocarcinoma (PDAC) is the fourth most common cause of cancerrelated deaths in United States and Europe. It is predicted that PDAC will become the second leading cause of cancer-related deaths during the next decades. The development of PDAC is not well understood, however, studies have shown that dysregulated exocrine pancreatic fluid secretion can contribute to pathologies of exocrine pancreas, including PDAC. The major roles of healthy exocrine pancreatic tissue are secretion of enzymes and bicarbonate rich fluid, where ion channels participate to fine-tune these biological processes. It is well known that ion channels located in the plasma membrane regulate multiple cellular functions and are involved in the communication between extracellular events and intracellular signaling pathways and can function as signal transducers themselves. Hereby, they contribute to maintain resting membrane potential, electrical signaling in excitable cells, and ion homeostasis. Despite their contribution to basic cellular processes, ion channels are also involved in the malignant transformation from a normal to a malignant phenotype. Aberrant expression and activity of ion channels have an impact on essentially all hallmarks of cancer defined as; uncontrolled proliferation, evasion of apoptosis, sustained angiogenesis and promotion of invasion and migration. Research indicates that certain ion channels are involved in the aberrant tumor growth and metastatic processes of PDAC. The purpose of this review is to summarize the important expression, localization, and function of ion channels in normal exocrine pancreatic tissue and how they are involved in PDAC progression and development. As ion channels are suggested to be potential targets of treatment they are furthermore suggested to be biomarkers of different cancers. Therefore, we describe the importance of ion channels in PDAC as markers of diagnosis and clinical factors.

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“…CCK activates the vagus nerve leading to cholinergic stimulation of acinar cells and consequent release of zymogen granules (35,37). Secretin and a decrease in duodenal pH from chyme cause ductal cells to release sodium, chloride, bicarbonate, and water into the pancreatic ducts via aquaporin and cystic fibrosis transmembrane conductance regular (CFTR) channels (37,38).…”

Section: Normal Physiology Of the Exocrine Pancreasmentioning

confidence: 99%