Aqueous biphasic systems composed of ionic liquids and polymers: A platform for the purification of biomolecules

Pereira, Jorge Fernando Brandão; Ventura, Sónia P. M.; Silva, Francisca A. e; Shahriari, Shahla; Freire, Mara G.; Coutinho, João A. P.

doi:10.1016/j.seppur.2013.04.004

Cited by 84 publications

(47 citation statements)

References 33 publications

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“…Water content in [Phpi] [BF 4 ] was less than 0.0001 of the mass fraction. 1 H and 13 C NMR analyses of the synthesized IL were carried out on a Bruker AVANCE III 500 MHz NMR spectrometer using D 2 O as the solvent. The NMR results are presented in Figures S1 and S2 in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

Formation of Novel Aqueous Two-Phase Systems with Piperazinium-Based Ionic Liquids and Anionic Surfactants: Phase Behavior and Microstructure

Lü

Cao

et al. 2015

J. Phys. Chem. B

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Two novel aqueous two-phase systems (ATPSs) involving protic piperazinium-based ionic liquids (ILs) and anionic surfactants were found in the 1-ethylpiperazinium tetrafluoroborate ([C2pi][BF4]) + sodium dodecyl sulfate (SDS) + H2O system and the 1-phenylpiperazinium tetrafluoroborate ([Phpi][BF4]) + sodium dodecyl benzenesulfonate (SDBS) + H2O system. The ATPS regions in the ternary phase diagrams were determined, and the compositions and the microstructures of the conjugated phases were analyzed by UV-vis, (1)H NMR, DLS, and cryogenic TEM measurements. The results demonstrate size-enhanced micelles for both ATPSs. The strong electrostatic interactions between the cationic moiety of IL and the anionic surfactant play a very important role in the assembly of the large aggregates, and the cation-π interactions are involved in the [Phpi][BF4] + SDBS + H2O ATPS. In addition, the small cationic moiety of [C2pi][BF4] can be packed in the micelles, while the larger hydrophilic cationic moiety of [Phpi][BF4] makes it difficult to get into the micelles, leading to the different size enhancement effects. The driving force of phase separation is the formation and distribution of the large aggregates in the aqueous solutions. This work presents a novel nonaromatic ATPS formed by a piperazinium-based IL and an anionic surfactant, in which considerable size enhancement of aggregates takes place without the assistance of aromaticity in contrast to the other aromatic ATPSs.

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Section: Methodsmentioning

confidence: 99%

Formation of Novel Aqueous Two-Phase Systems with Piperazinium-Based Ionic Liquids and Anionic Surfactants: Phase Behavior and Microstructure

Lü

Cao

et al. 2015

J. Phys. Chem. B

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“…Such mixtures are known as aqueous biphasic systems, as both layers are aqueous solutions of different compositions: the upper layer contains most of the PEG, whereas the lower layer contains most of the inorganic salt. Aqueous biphasic systems have proven to be useful in CCC for the purification of biological materials [121,[135][136][137]. Using ternary mass or molar phase diagrams, Berthod and coworkers studied the putative formation of ionic liquid-based aqueous biphasic systems by mixing [C 4 [21,22,24].…”

Section: Ionic Liquid-water Systemsmentioning

confidence: 99%

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

Brown¹,

Earle

Gîlea

et al. 2017

Topics in Current Chemistry Collections

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Ionic liquids can form biphasic solvent systems with many organic solvents and water, and these solvent systems can be used in liquid-liquid separations and countercurrent chromatography. The wide range of ionic liquids that can by synthesised, with specifically tailored properties, represents a new philosophy for the separation of organic, inorganic and bio-based materials. A customised countercurrent chromatograph has been designed and constructed specifically to allow the more viscous character of ionic liquid-based solvent systems to be used in a wide variety of separations (including transition metal salts, arenes, alkenes, alkanes, bio-oils and sugars).

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“…Moreover, a number of works have described novel cholinium-based ILs in which protein structure and the enzyme function can be maintained or even increased [47,49]. This fact, coupled with other advantages cited herein, motivated the application of these ILs to the formation of alternative ATPS, which served as a platform for the purification/separation of antibiotics [50,51] and proteins [47].…”

Section: Introductionmentioning

confidence: 98%

Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification

Souza

Lima

Coutinho

et al. 2015

Separation and Purification Technology

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Aqueous biphasic systems composed of ionic liquids and polymers: A platform for the purification of biomolecules

Cited by 84 publications

References 33 publications

Formation of Novel Aqueous Two-Phase Systems with Piperazinium-Based Ionic Liquids and Anionic Surfactants: Phase Behavior and Microstructure

Formation of Novel Aqueous Two-Phase Systems with Piperazinium-Based Ionic Liquids and Anionic Surfactants: Phase Behavior and Microstructure

Ionic Liquid–Liquid Chromatography: A New General Purpose Separation Methodology

Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification

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