Aqueous extract of Rhodiola imbricata rhizome stimulates Toll-like receptor 4, granzyme-B and Th1 cytokines in vitro

Mishra, K.P.; Ganju, Lilly; Chanda, Sunirmal; Karan, Dev; Sawhney, R. C.

doi:10.1016/j.imbio.2008.04.001

Cited by 33 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were stained with anti CD 16 FITC/56 PE (NK cell marker), anti CD 284 PE (TLR-4), anti CD253 (TREM-1) antibody (BD Pharmingen, San Jose, CA, USA). Samples were acquired using FACS caliber flow cytometer (Becton Dickinson, CA, USA) and analysed using Cell Quest Pro software [15].…”

Section: Flow Cytometric Analysis For Cell Surface Markersmentioning

confidence: 99%

Hypoxic Stress Induced TREM-1 and Inflammatory Chemokines in Human Peripheral Blood Mononuclear Cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

Hypoxia is a condition of low pO 2 , which creates a unique microenvironment affecting cell phenotype and subsequent immune response generation. Little is known about the impact of hypoxia on the phenotypic expression of NK cell, TREM-1, TLR-4 and inflammatory chemokines. In the present study we have determined the frequency of peripheral blood populations of CD16/CD56 (NK Cells) expressing cells, presence of activation marker CD354 (TREM-1), Toll like receptor (CD 284) on the cell surface and chemokines IL-8 and RANTES in the cellular supernatant of normoxia and hypoxia exposed cells by flow cytometry. GRP-78 expression was determined by reverse transcriptase polymerase chain reaction. The blood was collected from healthy individuals and exposed to normoxic and hypoxic (0.5 %) environment for 24 h. The percentage of NK cells (CD 16/56) was marginally up regulated while TLR-4 expression was diminished in hypoxia exposed cells as compare to the normoxic cells. TREM-1 expression was significantly up-regulated (p \ 0.05) in hypoxia as compared to the normoxic control. In addition when monocytic cell line THP-1 was exposed to 0.5 % hypoxia for 24 h, TLR4 expression was significantly decreased in hypoxic cells as compared to normoxic cells. Furthermore, GRP-78 mRNA expression was also upregulated by hypoxia or LPS exposure. These events are paralleled by strengthening up-regulation of the chemokines IL-8 and RANTES an otherwise necessary event for the chemotaxis of the neutrophils and macrophages to the inflammatory site. In conclusion, this study provides a novel insight into the mechanism linking low oxygen tension to the regulation of immune and inflammatory responses, leading to new perspectives of the role of hypoxia in programming immune cell functions.

show abstract

Section: Flow Cytometric Analysis For Cell Surface Markersmentioning

confidence: 99%

Hypoxic Stress Induced TREM-1 and Inflammatory Chemokines in Human Peripheral Blood Mononuclear Cells

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Further, it was observed that RAE treatment induced toll like receptor-4 (TLR-4) and intracellular granzyme B expression in mouse splenocytes [6]. The RAE decreased the proliferation of human leukemic cell lines K-562, HL-60 and EL-4.…”

Section: Introductionmentioning

confidence: 99%

“…The rhizome mostly contains rosin, rosavin, p-tyrosol and gallic acid [5][6][7]. Experimental exploration of Rhodiola rhizome has not explored in detail [3].…”

Section: Introductionmentioning

confidence: 99%

Adjuvant effect of aqueous extract ofRhodiola imbricatarhizome on the immune responses to tetanus toxoid and ovalbumin in rats

Mishra

Chanda

Shukla

et al. 2009

Immunopharmacology and Immunotoxicology

Self Cite

View full text Add to dashboard Cite

In the present study we have evaluated the immunopotentiating activity of Rhodiola aqueous extract (RAE) in rats. The efficacy of RAE was determined by using strong antigen tetanus toxoid (TT) and weak antigen Ovalbumin (OVA). The dynamic changes in humoral and cell-mediated immune response were measured. The results indicated that the TT specific immunoglobulin levels were significantly enhanced by RAE and were at par with complete Freund's adjuvant (CFA). The level of OVA induced antibody response was also enhanced by RAE. It was observed that TT and OVA in combination with CFA or RAE could evoke a significant delayed type hypersensitivity (DTH) response, confirming its potential to generate strong cell-mediated immunity (CMI). The anti-inflammatory or immunosuppressive effect of RAE was evaluated in adjuvant-induced arthritis model (AIA). RAE could not suppress the swelling response. Therefore, this study suggests that RAE has adjuvant/immunopotentiating activity in terms of humoral as well as cell-mediated immune response against strong antigen like TT and weak antigen like OVA.

show abstract

“…Not limited to pathogen-associated molecular patterns (PAMP), TLR4 responds to human endogenous structural proteins derived from tissue injury or during inflammation, the damage-associated molecular patterns (DAMP), such as type III repeat extra domain A of fibronectin, oligosaccharides of hyaluronic acid, human heat-shock protein Hsp60 and Hsp70 (see review [59]). A few reports have revealed the potential for protein extracts from plants and herbs to activate TLR4, such as taxol, an antitumor agent derived from the Yew plant [60], and aqueous extract of Rhodiola imbricata rhizome, a medicinal plant [61].…”

Section: Cutting Edge Breakthrough: Short Ragweed Pollen Triggers Ocumentioning

confidence: 99%

Recent Advances in Mucosal Immunology and Ocular Surface Diseases

Li¹,

Liu²,

Li³

et al. 2012

Advances in Ophthalmology

View full text Add to dashboard Cite

Aqueous extract of Rhodiola imbricata rhizome stimulates Toll-like receptor 4, granzyme-B and Th1 cytokines in vitro

Cited by 33 publications

References 8 publications

Hypoxic Stress Induced TREM-1 and Inflammatory Chemokines in Human Peripheral Blood Mononuclear Cells

Hypoxic Stress Induced TREM-1 and Inflammatory Chemokines in Human Peripheral Blood Mononuclear Cells

Adjuvant effect of aqueous extract ofRhodiola imbricatarhizome on the immune responses to tetanus toxoid and ovalbumin in rats

Recent Advances in Mucosal Immunology and Ocular Surface Diseases

Contact Info

Product

Resources

About