Aqueous extracts of lingonberry and blackberry leaves identified by high-content screening beneficially act on cholesterol metabolism

Röhrl, Clemens; Steinbauer, Stefanie; Bauer, Raimund; Roitinger, Eva; Otteneder, Katharina; Wallner, Melanie; Neuhauser, Cathrina; Schwarzinger, Bettina; Schwarzinger, Clemens; Stangl, Herbert; Iken, Marcus; Weghuber, Julian

doi:10.1039/d1fo01169c

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…Our experimental material lingonberry ( Figure 1 ) is a red berry, also known as mountain cranberry, cowberry. Sweet and sour taste, leaves and fruits can treat urethritis, cystitis, enteritis, dysentery and other diseases ( Kowalska, 2021 ; Parnanen et al, 2021 ; Rohrl et al, 2021 ). These berries are mainly developed and utilized in the United States and European countries.…”

Section: Discussionmentioning

confidence: 99%

Lingonberry (Vaccinium vitis-idaea L.) Interact With Lachnum pygmaeum to Mitigate Drought and Promote Growth

et al. 2022

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The application of Ericoid mycorrhizal (ErM) fungi is considered to be an important strategy for increasing plant yield and drought resistance. In this study, we isolated and identified two ErM fungi that can promote the growth of lingonberry. We tried to understand the potential of these two ErM fungi to promote the growth of lingonberry and the strategies to help plants cope with water shortage. The use value of ErM fungi was evaluated by inoculating Oidiodendron maius FC (OmFC) or Lachnum pygmaeum ZL6 (LpZL6), well-watered (WW) and severe drought stress (SDS). The results showed that the mycelium of LpZL6 was denser than that of OmFC, and both ErM fungi significantly increased the biomass of lingonberry stems and roots. They also significantly increased the chlorophyll content by 65.6 and 97.8%, respectively. In addition, inoculation with LpZL6 fungi can improve drought resistance, promote root growth and increase root wet weight by 1157.6%. Drought reduced the chlorophyll content and soluble sugar content of lingonberry but increased significantly after inoculation with LpZL6. Inoculation with LpZL6 decreased lingonberry’s malondialdehyde (MDA) content but increased the superoxide dismutase (SOD) activity. Overall, these results indicated that the successful coexistence of ErM fungi and lingonberry alleviated the adverse effects of drought stress through higher secondary metabolites and photosynthetic pigment synthesis.

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Section: Discussionmentioning

confidence: 99%

Lingonberry (Vaccinium vitis-idaea L.) Interact With Lachnum pygmaeum to Mitigate Drought and Promote Growth

et al. 2022

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“…Cellular free and esterified cholesterol were measured by gas chromatography as previously described 15 . Briefly, cells were washed and detached by scraping in ice-cold PBS.…”

Section: Methodsmentioning

confidence: 99%

Elder (Sambucus nigra), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis

Steinbauer,

König,

Neuhauser

et al. 2024

Sci Rep

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Cholesterol deposition in intimal macrophages leads to foam cell formation and atherosclerosis. Reverse cholesterol transport (RCT), initiated by efflux of excess cholesterol from foam cells, counteracts atherosclerosis. However, targeting RCT by enhancing cholesterol efflux was so far accompanied by adverse hepatic lipogenesis. Here, we aimed to identify novel natural enhancers of macrophage cholesterol efflux suitable for the prevention of atherosclerosis. Plant extracts of an open-access library were screened for their capacity to increase cholesterol efflux in RAW264.7 macrophages trace-labeled with fluorescent BODIPY-cholesterol. Incremental functional validation of hits yielded two final extracts, elder (Sambucus nigra) and bitter orange (Citrus aurantium L.) that induced ATP binding cassette transporter A1 (ABCA1) expression and reduced cholesteryl ester accumulation in aggregated LDL-induced foam cells. Aqueous elder extracts were subsequently prepared in-house and both, flower and leaf extracts increased ABCA1 mRNA and protein expression in human THP-1 macrophages, while lipogenic gene expression in hepatocyte-derived cells was not induced. Chlorogenic acid isomers and the quercetin glycoside rutin were identified as the main polyphenols in elder extracts with putative biological action. In summary, elder flower and leaf extracts increase macrophage ABCA1 expression and reduce foam cell formation without adversely affecting hepatic lipogenesis.

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“…HPLC‐MS analyses were performed by reversed‐phase chromatography using a Surveyor HPLC (Thermo Fisher Scientific) equipped with an Accucore C18 column (150 mm × 3.0 mm i.d., 2.6 µm particle size; Thermo Fisher Scientific) as described. [ 33 ] High‐resolution mass spectra were obtained using an LTQ Orbitrap Velios (Thermo Fisher Scientific) with an APCI source operated in positive and negative ionization mode. The resolution was set to 30 000 and diisooctylphthalate ( m / z = 391.2843) was used as an internal standard for mass calibration.…”

Section: Methodsmentioning

confidence: 99%

Anti‐Hyperglycemic Effects of Oils and Extracts Derived from Sea Buckthorn – A Comprehensive Analysis Utilizing In Vitro and In Vivo Models

Ollinger¹,

Neuhauser

Schwarzinger

et al. 2022

Molecular Nutrition Food Res

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Scope Sea buckthorn (Hippophaes rhamnoides) is capable of ameliorating disturbed glucose metabolism in animal models and human subjects. Here, the effect of sea buckthorn oil as well as of extracts of fruits, leaves, and press cake on postprandial glucose metabolism is systematically investigated. Methods and results Sea buckthorn did neither exert decisive effects in an in vitro model of intestinal glucose absorption nor did it alter insulin secretion. However, sea buckthorn stimulates GLUT4 translocation to the plasma membrane comparable to insulin, indicative of increased glucose clearance from the circulation. Isorhamnetin is identified in all sea buckthorn samples investigated and is biologically active in triggering GLUT4 cell surface localization. Consistently, sea buckthorn products lower circulating glucose by ≈10% in a chick embryo model. Moreover, sea buckthorn products fully revert hyperglycemia in the nematode Caenorhabditis elegans while they are ineffective in Drosophila melanogaster under euglycemic conditions. Conclusion These data indicate that edible sea buckthorn products as well as by‐products are promising resources for hypoglycemic nutrient supplements that increase cellular glucose clearance into target tissues.

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Aqueous extracts of lingonberry and blackberry leaves identified by high-content screening beneficially act on cholesterol metabolism

Abstract: Lingonberry and blackberry leaf extracts alter cholesterol metabolism and potentially protect from atherosclerosis.

Cited by 7 publications

References 32 publications

Lingonberry (Vaccinium vitis-idaea L.) Interact With Lachnum pygmaeum to Mitigate Drought and Promote Growth

Lingonberry (Vaccinium vitis-idaea L.) Interact With Lachnum pygmaeum to Mitigate Drought and Promote Growth

Elder (Sambucus nigra), identified by high-content screening, counteracts foam cell formation without promoting hepatic lipogenesis

Anti‐Hyperglycemic Effects of Oils and Extracts Derived from Sea Buckthorn – A Comprehensive Analysis Utilizing In Vitro and In Vivo Models

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