Aqueous Humor Cytokine Response in the Contralateral Eye after First-Eye Cataract Surgery in Patients with Primary Angle-Closure Glaucoma, High Myopia or Type 2 Diabetes Mellitus

Tang, Jiancen; Liu, Hanhan; Sun, Mengsha; Zhang, Xueqian; Chen, Hao; Li, Qian; Prokosch, Verena; Cui, Hongping

doi:10.31083/j.fbl2707222

Cited by 9 publications

(6 citation statements)

References 32 publications

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“…Through the analysis of aqueous humor in patients with diabetes, high myopia, and primary angle-closure glaucoma, Jiancen Tang and colleagues discovered that IL-2 levels in the aqueous humor of the contralateral eye increased compared to the preoperative levels in the operated eye after surgery. This phenomenon was also con rmed in our animal experiments [30] .It is well known that IL-2, produced by T cells, is an immunoregulatory protein. IL-2 plays a crucial role in regulating the immune system, particularly in the proliferation, differentiation, and function of T cells.…”

Section: Discussionsupporting

confidence: 88%

“…Interestingly, in recent years, more and more teams have found that in non-diabetic patients, the aforementioned phenomenon and high expression of biological factors such as MCP-1 do not exist [28,29] .In diabetic patients, there is a signi cant increase in MCP-1 and substance P in the second eye [28,29] . Furthermore, when different diseases are combined, the expression of biological factors in the aqueous humor before and after surgery varies among patients [30] .Therefore, in patients with systemic diseases, there are inherent differences in the AH environment.…”

Section: Introductionmentioning

confidence: 99%

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Changes in AH cytokines and metabolomics in contralateral eyes of ARC patients following unilateral surgery reveal effects on the contralateral eye's intraocular environment stability

Li,

Cheng,

Zhou

et al. 2024

Preprint

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Background: For patients with bilateral age-related cataracts, sequential phacoemulsification and intraocular lens implantation is a common treatment. However, it remains unclear whether surgery on the first eye affects the outcome of the second eye, as current research results are inconsistent. This study will explore whether surgery on one eye affects the non-operated eye by analyzing changes in aqueous humor biomarkers and metabolomics in the second eye. Methods: A rabbit model of unilateral phacoemulsification and intraocular lens implantation was established. The experimental group consisted of 15 rabbits undergoing this procedure. Postoperatively, rabbits were divided into five subgroups (three rabbits per subgroup), and aqueous humor was collected from both the operated and non-operated eyes at 1 day, 3 days, 1 week, 2 weeks, and 3 weeks after surgery. Additionally, 5 rabbits were selected as a control group, from which aqueous humor was extracted. Levels of IL-1a, IL-1β, IL-2, IL-4, IL-6, IL-8, IFN-γ, TNF-α, MCP-1, and VEGF in the aqueous humor were compared. In the clinical study, preoperative aqueous humor samples were collected from 22 patients undergoing bilateral phacoemulsification and intraocular lens implantation. Among them, 11 patients were tested for the aforementioned 10 cytokines, while the other 11 patients underwent untargeted metabolomics research. Results: In the animal experiment, levels of all 10 cytokines in the operated eyes were significantly higher compared to both the control and non-operated eyes groups (P < 0.05). In the non-operated eyes, IL-1β and IL-2 levels were also elevated compared to the control (P < 0.05). IL-1β remained elevated at 3 weeks post-surgery, while IL-2 returned to baseline after 3 days.In the clinical study, no significant differences were found in cytokine levels between the two eyes.In the untargeted metabolomics analysis, 354 metabolites showed differential expression—280 were upregulated and 74 were downregulated. Notably, Adenine and 2-Aminopurine were significantly downregulated, highlighting Purine metabolism as the most impacted pathway. Conclusions: Animal experiments showed a significant increase in IL-1β and IL-2 levels in the non-operated eyes postoperatively, reflecting systemic and local inflammatory responses. In clinical experiments, although no significant changes in cytokines were observed in the aqueous humor of both eyes, differential expression of metabolites indicated metabolic adjustments in the non-operated eye following surgery on the first eye. These findings suggest potential metabolic effects of surgery on the non-operated eye at the metabolic level.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Changes in AH cytokines and metabolomics in contralateral eyes of ARC patients following unilateral surgery reveal effects on the contralateral eye's intraocular environment stability

Li,

Cheng,

Zhou

et al. 2024

Preprint

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“…IgG N-glycome profiling by matrix-assisted laser desorption/ ionization time of flight mass spectrometry, raw matrix-assisted laser desorption/ionization time of flight mass spectrometry data processing, and circulating inflammatory markers measurement (cytokine assay) were conducted according to previous reports 16,[20][21][22][23] and are described in detail in the Supplemental Material.…”

Section: Mass Spectrometric Igg N-glycomics and Cytokine Assaymentioning

confidence: 99%

Human Plasma IgG N -Glycome Profiles Reveal a Proinflammatory Phenotype in Chronic Thromboembolic Pulmonary Hypertension

et al. 2023

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BACKGROUND: The pathological mechanism of chronic thromboembolic pulmonary hypertension (CTEPH) is not fully understood, and inflammation has been reported to be one of its etiological factors. IgG regulates systemic inflammatory homeostasis, primarily through its N -glycans. Little is known about IgG N -glycosylation in CTEPH. We aimed to map the IgG N -glycome of chronic thromboembolic pulmonary hypertension to provide new insights into its pathogenesis and discover novel markers and therapies. METHODS: We characterized the plasma IgG N -glycome of patients with chronic thromboembolic pulmonary hypertension in a discovery cohort and validated our results in an independent validation cohort using matrix-assisted laser desorption/ionization time of flight mass spectrometry. Thereafter, we correlated IgG N -glycans with clinical parameters and circulating inflammatory cytokines in patients with CTEPH. Furthermore, we determined IgG N -glycan quantitative trait loci in chronic thromboembolic pulmonary hypertension to reveal partial mechanisms underlying glycan changes. RESULTS: Decreased IgG galactosylation representing a proinflammatory phenotype was found in CTEPH. The distribution of IgG galactosylation showed a strong association with NT-proBNP (N-terminal pro-B-type natriuretic peptide) in CTEPH. In line with the glycomic findings, IgG pro-/anti-inflammatory N -glycans correlated well with a series of inflammatory markers and gene loci that have been reported to be involved in the regulation of these glycans or inflammatory immune responses. CONCLUSIONS: This is the first study to reveal the full signature of the IgG N -glycome of a proinflammatory phenotype and the genes involved in its regulation in CTEPH. Plasma IgG galactosylation may be useful for evaluating the inflammatory state in patients with CTEPH; however, this requires further validation. This study improves our understanding of the mechanisms underlying chronic thromboembolic pulmonary hypertension inflammation from the perspective of glycomics.

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“…Inflammatory marker measurements were performed without knowledge of the CHIP status of the patients. Profiling of the inflammatory marker of circulating cytokines and IgG galactosylation (IgG-Gal) ratio was performed according to published protocols, [27][28][29] detailed methods were in Supplemental Material.…”

Section: Inflammatory Marker Measurementsmentioning

confidence: 99%

Clonal Hematopoiesis of Indeterminate Potential in Chronic Thromboembolic Pulmonary Hypertension: A Multicenter Study

Liu,

Zhou,

Lian

et al. 2024

Hypertension

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BACKGROUND: The pathogenesis of chronic thromboembolic pulmonary hypertension (CTEPH) is multifactorial and growing evidence has indicated that hematological disorders are involved. Clonal hematopoiesis of indeterminate potential (CHIP) has recently been associated with an increased risk of both hematological malignancies and cardiovascular diseases. However, the prevalence and clinical relevance of CHIP in patients with CTEPH remains unclear. METHODS: Using stepwise calling on next-generation sequencing data from 499 patients with CTEPH referred to 3 centers between October 2006 and December 2021, CHIP mutations were identified. We associated CHIP with all-cause mortality in patients with CTEPH. To provide insights into potential mechanisms, the associations between CHIP and inflammatory markers were also determined. RESULTS: In total, 47 (9.4%) patients with CTEPH carried at least 1 CHIP mutation at a variant allele frequency of ≥2%. The most common mutations were in DNMT3A , TET2 , RUNX1 , and ASXL1 . During follow-up (mean, 55 months), deaths occurred in 22 (46.8%) and 104 (23.0%) patients in the CHIP and non-CHIP groups, respectively ( P <0.001, log-rank test). The association of CHIP with mortality remained robust in the fully adjusted model (hazard ratio, 2.190 [95% CI, 1.257–3.816]; P =0.006). Moreover, patients with CHIP mutations showed higher circulating interleukin-1β and interleukin-6 and lower interleukin-4 and IgG galactosylation levels. CONCLUSIONS: This is the first study to show that CHIP mutations occurred in 9.4% of patients with CTEPH are associated with a severe inflammatory state and confer a poorer prognosis in long-term follow-up.

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Aqueous Humor Cytokine Response in the Contralateral Eye after First-Eye Cataract Surgery in Patients with Primary Angle-Closure Glaucoma, High Myopia or Type 2 Diabetes Mellitus

Cited by 9 publications

References 32 publications

Changes in AH cytokines and metabolomics in contralateral eyes of ARC patients following unilateral surgery reveal effects on the contralateral eye's intraocular environment stability

Changes in AH cytokines and metabolomics in contralateral eyes of ARC patients following unilateral surgery reveal effects on the contralateral eye's intraocular environment stability

Human Plasma IgG N -Glycome Profiles Reveal a Proinflammatory Phenotype in Chronic Thromboembolic Pulmonary Hypertension

Clonal Hematopoiesis of Indeterminate Potential in Chronic Thromboembolic Pulmonary Hypertension: A Multicenter Study

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