Aqueous Humor Nitric Oxide Levels in Patients With Behçet Disease

Yılmaz, Gürsel; Sizmaz, Sadi; Demirel-Yılmaz, Emine; Duman, Sunay; Aydin, Pınar

doi:10.1097/00006982-200206000-00012

Cited by 40 publications

(21 citation statements)

References 38 publications

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“…Behcet's disease presents clinical symptoms of uveitis. Yilmaz et al (2002) have recently detected an increase in total nitrate in the aqueous humor of patients (2.1±0.6 μM) compared with levels detected in nonsufferers (>0.08 μM). Similarly, Ohta and colleagues (2003) have shown an increase of nitrate levels from 15.4 μM to 57.1±1.7 μM in the aqueous humor of experimentally lipopolysaccharide (LPS)-induced uveitis in rats.…”

Section: Oxidative Stress and Lipid-peroxidationmentioning

confidence: 95%

Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells

Serbecic

Beutelspacher

2005

Cell Tissue Res

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To determine the effects of vitamin supplementation on the lipid-peroxidation-mediated toxicity of iron-ions on corneal endothelial cells (CECs) leading to apoptosis, murine CECs were maintained in tissue culture medium supplemented with increasing concentrations of free iron-ions, a treatment known to lead to increased lipid-peroxidation. The concentration of anti-oxidative vitamins (ascorbic acid, tocopherol and retinoic acid) in the cell supernatant and in the cells was determined by high-pressure liquid chromatography. Apoptosis was assessed by quantification of caspase-3-like activity and by using annexin-V/propidium iodide stains for flow cytometry. Lipid-peroxidation was measured by the malondialdehyde method. Supplementation with anti-oxidative vitamins was tested for the ability to counteract the induction of apoptosis. The production of nitric oxide was assessed spectrophotometrically and the expression levels of inducible and endothelial nitric oxide synthase were determined by Western blot. Increasing levels of free iron led to a rapid loss of anti-oxidative vitamins in the supernatant and in the CECs. This was correlated with rising levels of malondialdehyde and increased apoptosis. Supplementation with ascorbic acid or alpha-tocopherol alone did not prevent lipid-peroxidation in the cells. A combination of vitamins C and E (ascorbic acid, tocopherol) or solitary supplementation with vitamin A (retinoic acid) prevented lipid-peroxidation. We thus present a novel in vitro model for testing the direct influence of pro-oxidative species on CECs. We also show that supplementation with anti-oxidative vitamins to CECs significantly prevents the generation of free-radical-induced oxidative injury and apoptosis. These findings may have important implications for the storage of human corneae prior to transplantation and for the prolongation of corneal graft survival.

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Section: Oxidative Stress and Lipid-peroxidationmentioning

confidence: 95%

Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells

Serbecic

Beutelspacher

2005

Cell Tissue Res

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“…Uveitis is a devastating inflammatory eye disease that can lead to visual impairment and blindness 1 – 3. There is accumulating evidence that nitric oxide (NO) plays a role in the development of experimental and clinical uveits 1 – 3.…”

mentioning

confidence: 99%

“…There is accumulating evidence that nitric oxide (NO) plays a role in the development of experimental and clinical uveits 1 – 3. Indeed, aqueous humor levels of NO metabolites are increased in animal models and patients with uveitis 1 – 3…”

mentioning

confidence: 99%

Aqueous humor levels of asymmetric dimethylarginine (ADMA) are positively associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis

Yoshida

Yamagishi²,

Ueda³

et al. 2007

British Journal of Ophthalmology

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“…NO is an important mediator of uveal inflammation and NO synthase activity has been demonstrated experimentally in the uveal tissue [11]. In addition, aqueous humor NO levels have been reported to be increased in uveitic BD patients [31]. Therefore, when patients with ocular involvement are compared to the control group, this statistical significance becomes sharper.…”

Section: Discussionmentioning

confidence: 99%

Angiotensin-converting enzyme gene and endothelial nitric oxide synthase gene polymorphisms in Behçet’s disease with or without ocular involvement

et al. 2009

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Aqueous Humor Nitric Oxide Levels in Patients With Behçet Disease

Abstract: Aqueous humor nitric oxide levels are elevated in patients with a history of Behçet disease.

Cited by 40 publications

References 38 publications

Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells

Anti-oxidative vitamins prevent lipid-peroxidation and apoptosis in corneal endothelial cells

Aqueous humor levels of asymmetric dimethylarginine (ADMA) are positively associated with monocyte chemoattractant protein-1 (MCP-1) in patients with uveitis

Angiotensin-converting enzyme gene and endothelial nitric oxide synthase gene polymorphisms in Behçet’s disease with or without ocular involvement

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