Aqueous Infrared Pharmaceutical Analysis of Two Choline Compounds by Flow Injection Analysis Using the CIRCLE Cell

Abstract

The advent of the cylindrical internal reflectance (CIRCLE) cell has solved the difficult problem of quantitation of solutes in aqueous solutions by infrared spectroscopy. By a combination of flow injection analysis (FIA) and Fourier transform infrared (FT-IR) spectroscopy with the CIRCLE cell, a fast, reproducible technique suited for the determination of two choline compounds of pharmaceutical interest—succinylcholine chloride and bethanechol chloride—was developed. Quantitation was carried out with the use of either the ester band at 1075 cm−1 for bethanechol or the trimethyl quaternary ammonium band at 953 cm−1 for succinylcholine. Linearity of standard solutions was achieved from about 0.5 to at least 50 ppt for both compounds, and detection limits of 0.02% w/v were possible. A sample throughput of 60 samples/h was estimated. Analysis of pharmaceutical preparations was performed after minor sample clean-up, which was necessary only in the case of the bethanechol chloride tablets.

Determination of diosmin in pharmaceutical formulations using Fourier transform infrared spectrophotometry

“…Infrared spectrometry (IR) provides a useful way for the identification of drugs (USP XXII, 1990;Moffat, 1986;Ciurczak and Drennen, 2001;McClure, 1992;Garrigues et al, 1992;Miller et al, 1988) as well as for quantitative analysis, and the help of Fourier transform (FT-IR) permits continuous monitoring of the spectral baseline and simultaneous analysis of different components of the same sample.…”

FT-IR Spectrophotometric Analysis of Ascorbic Acid and Biotin and their Pharmaceutical Formulations

“…However, the traditional techniques employed to obtain the IR spectra, such as alkali halides disks, mulls, and thin films, are sometimes not adequate for quantitative analysis. Fourier transform (FT)-IR permits continuous monitoring of the spectral baseline and simultaneous analysis of different components of the same sample (Garrigues, Gallignani, and de la Guardia 1992;Miller, Danielson, and Katon 1988).…”

Whey fermentation: on-line analysis of lactose and lactic acid by FTIR spectroscopy

“…Braue and Pannella (1987) have suggested that the reliable sensitivity limit in the analysis of aqueous solutions is 0.05% (w/v). Miller et al (1988) however, claimed that detection limits of 0.02% (w/v) were possible, thereby supporting the specified limits of the CIR-CLE cell. 303 Figure 2 shows the change in the MIR spectrum of the medium during the course of fermentation.…”