Aqueous two-phase affinity partitioning systems: Current applications and trends

Ruiz‐Ruiz, Federico; Benavides, Jorge; Aguilar, Oscar; Rito‐Palomares, Marco

doi:10.1016/j.chroma.2012.04.077

Cited by 142 publications

(72 citation statements)

References 68 publications

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“…This method has advantages over conventional extraction techniques like, environment-friendly, low cost, capable of continuous operation, ease of scaling-up and is efficient for many kinds of experiments specially for the concentration and purification of biomolecules [1, 2, 4, 5]. The use of affinity ligands in ATPS can result in the higher recovery yields and higher purification folds of biological products as it is a primary stage recovery technique [6]. Affinity ligands can be covalently attached to polymer or polymer can also be modified with hydrophobic groups [5] Interested readers about aqueous two-phase affinity partitioning (ATPAP) are referred to an excellent review by Ruiz-Ruiz et al [6].…”

Section: History and Backgroundmentioning

confidence: 99%

“…The use of affinity ligands in ATPS can result in the higher recovery yields and higher purification folds of biological products as it is a primary stage recovery technique [6]. Affinity ligands can be covalently attached to polymer or polymer can also be modified with hydrophobic groups [5] Interested readers about aqueous two-phase affinity partitioning (ATPAP) are referred to an excellent review by Ruiz-Ruiz et al [6]. …”

Section: History and Backgroundmentioning

confidence: 99%

“…Other types include, ionic liquids and short-chain alcohols [1, 2, 4, 6, 7, 10, 11]. In addition to this, ionic and/or non-ionic surfactants are used for the formation of micellar and reverse micellar ATPSs [6, 12, 13]. Polymer – polymer/salt systems have been studied for more than five decades.…”

Section: Types Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

“…Downstream processing can cost up to 80 % of the overall production cost [94] and phase separation by gravity in the downstream process is time consuming, which ultimately lead to an escalating production costs [6, 87, 97, 98]. To overcome this limitation column extractor are used which do not require centrifugation for phase separation.…”

Section: Applications Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

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Aqueous two-phase system (ATPS): an overview and advances in its applications

et al. 2016

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Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia. Although, the partition behavior involved in the method is complex and difficult to predict. Current research shows that it has also been successfully used in the detection of veterinary drug residues in food, separation of precious metals, sewage treatment and a variety of other purposes. The ATPS is able to give high recovery yield and is easily to scale up. It is also very economic and environment friendly method. The aim of this review is to overview the basics of ATPS, optimization and its applications.

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Section: History and Backgroundmentioning

confidence: 99%

Section: History and Backgroundmentioning

confidence: 99%

Section: Types Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

Section: Applications Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

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Aqueous two-phase system (ATPS): an overview and advances in its applications

et al. 2016

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“…The transfer of a solute from one phase to the other phase in the ATPSs depends strongly on the components within the system, as well as, of the intensive thermodynamic properties of the two phases in equilibrium [19]. In this sense, modulating these thermodynamic properties is fundamental in order to obtain the best conditions for the purification of solutes and the creation of news ATPSs.…”

Section: Introductionmentioning

confidence: 99%

Phase diagrams, densities and refractive indexes of poly(ethylene oxide)+organic salts+water aqueous two-phase systems: Effect of temperature, anion and molar mass

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Ferreira

et al. 2015

Fluid Phase Equilibria

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Phase diagrams, densities and refractive indexes of poly(ethylene oxide) + organic salts + water aqueous two-phase systems: Effect of temperature, anion and molar mass, Fluid Phase Equilibria http://dx. Graphical abstractHighlights  The segregation process is endothermic and entropically driven for all ATPS.  The biphasic region on the phase diagrams increase as the molar mass of PEO increase.  The biphasic region increased in relation to the anions studied: citrate > tartrate > succinate. AbstractThe application of aqueous two-phase systems (ATPSs) at the industrial level requires systems formed by non-toxic substances to decrease the negative impact on the environment. Organic salts such as sodium citrate, sodium tartrate and sodium succinate have been utilized in order to fulfill this objective. In this work, ATPSs formed by poly(ethylene oxide), PEO, with molar mass 10,000 or 35,000 g mol -1 , organic salts and water, namely PEO10000 + sodium citrate + water, PEO10000 + sodium tartrate + water, PEO10000 + sodium succinate + water and PEO35000 + sodium citrate + water ATPSs at (283.15, 298.15 and 313.15) K have been studied. Effects of temperature, anion and polymer molar mass on the phase diagrams, as well as, the densities and refractive indexes of both phases of the ATPSs were evaluated. The segregation process was endothermic and entropically driven for all ATPSs. The biphasic region on the phase diagrams increased as the molar mass of PEO increased. In addition, the biphasic region also increased in relation to the anions studied: citrate > tartrate > succinate. The consistency of the tie-line data was ascertained by applying the Othmer-Tobias correlation.

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