Aqueous two-phase partition of complex protein feedstocks derived from brain tissue homogenates

Aqueous two-phase recovery of bio-nanoparticles: A miniaturization study for the recovery of bacteriophage T4

“…The partition behavior of bioproducts previously purified differs from that when such systems are challenged with crude or partially purified feedstock. This effect has been reported previously where the partition of biological suspensions had an impact upon the performance of the ATPS [23,30].…”

Practical application of aqueous two-phase partition to process development for the recovery of biological products

“…65%) produced by recombinant E. coli was reported using an one-stage ATPS process [16]. These reports and others [2][3][4][5][6][7][8][9][10][11][12][13][14][15] clearly demonstrated the benefits of ATPS processes at bench scale for the recovery of products. Furthermore, the type of proteins products that have been recovered with acceptable process yield (i.e.…”

“…A critical analysis of reports [2][3][4][5][6][7][8][9][10][11][12][13][14][15] dealing with the use of ATPS in bioproducts recovery exhibited that the application of ATPS for the recovery of proteins has resulted in processes designed as primary purification operations. Such processes have been characterised by single or multi-staged operations.…”

(Surfactant+polymer) interaction parameter studied by (liquid+liquid) equilibrium data of quaternary aqueous solution containing surfactant, polymer, and salt

“…There are three kinds of ATPSs containing (polymer + polymer) ATPS, (polymer + salt) ATPS, and (surfactant + salt) ATPS that have been used for partitioning of biological materials. (Polymer + polymer) and (polymer + salt) ATPSs are well-known [4][5][6][7][8][9][10] but investigations on (surfactant + salt) ATPS scarcely have been found in the literature. Recently Wang et al [11] have used ATPS containing surfactant Triton-X 100 and some salts for partitioning of membrane proteins.…”