Aqueous two-phase systems with thermo-sensitive EOPO co-polymer (UCON) and sulfate salts: Effect of temperature and cation

Rico, Xiana; González-Amado, Marlen; Soto, Ana; Rodrı́guez, Oscar

doi:10.1016/j.jct.2017.01.009

Cited by 20 publications

(8 citation statements)

References 21 publications

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“…Parallelly, the effect of electrolytes might also induce changes in the solution properties, i.e. causing salting in and salting out effects [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives

Sabo

Lopes

Santos-Ebinuma

et al. 2018

Process Biochemistry

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The partitioning of bacteriocin, a promising alternative to chemical preservatives, in a novel inexpensive and stable aqueous two-phase system (ATPS) comprising poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) was studied. The ATPS was generated by mixing both polymers with Na 2 SO 4 or choline chloride ([Ch]Cl) and a bacteriocin extract from the fermented broth of Lactobacillus plantarum ST16Pa. Bacteriocin showed stability at different pH values (3.0-8.0) and temperatures (50-80°C), as well as in the presence of ATPS components. Hydrophobic and electrostatic interactions were found to be the major driving forces for bacteriocin partitioning. The peptide partitioned preferentially to the PEG-rich phase (partition coefficient, K Bact > 1). However, the highest partition coefficient was achieved in the polymeric-based ATPS using [Ch]Cl as additive, as follows: 8 wt% PEG 10,000 g/mol/8 wt% NaPA 8,000 g/mol/0.5 M [Ch]Cl, resulting in a K Bact equal to 32. Moreover, these conditions promoted high selectivity (S = 62.7), since the greater part of total proteins partitioned into the NaPA-rich phase (K prot = 0.51). In conclusion, ATPS composed of PEG/NaPA using cholinebased salts as additive can be considered mainly as a first step for bacteriocin extraction from fermented broth by applying low polymer content and mild conditions.

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“…Parallelly, the effect of electrolytes might also induce changes in the solution properties, i.e. causing salting in and salting out effects [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives

Sabo

Lopes

Santos-Ebinuma

et al. 2018

Process Biochemistry

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“… 35 Compared with the two-phase region of Na 3 C 6 H 5 O 7 and K 3 C 6 H 5 O 7 , it was found that the salting-out ability of cations follows the order: Na + > K + for the citrate-based salts, which is accordant with the literature. 50 Similarly, the absolute values of Δ hyd G and Δ hyd S for Na + and K + are in the order Na + (Δ hyd G = −365 kJ mol −1 , Δ hyd S = −111 J K −1 mol −1 ) > K + (Δ hyd G = −295 kJ mol −1 , Δ hyd S = −74 J K −1 mol −1 ). The effect of cations on the salting-out ability for cholinium-based MILs follows the Hofmeister series.…”

Section: Resultsmentioning

confidence: 95%

“…35 Compared with the two-phase region of Na 3 C 6 H 5 O 7 and K 3 C 6 H 5 O 7 , it was found that the saltingout ability of cations follows the order: Na + > K + for the citratebased salts, which is accordant with the literature. 50 Similarly, the absolute values of D hyd G and D hyd S for Na + and K + are in the order Na…”

Section: Resultsmentioning

confidence: 98%

Extraction in cholinium-based magnetic ionic liquid aqueous two-phase system for the determination of berberine hydrochloride in Rhizoma coptidis

et al. 2018

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“…Effect of concentration of UCON solution and phosphate solution on the separation of ⊍-La From previous research, the ATPF demonstrated a stable existence when the UCON content was 40-80%. 34 An excessive UCON concentration led to high viscosity, which was not conducive to flotation. Therefore, relatively low concentrations of UCON solution (30%, 40% and 50%, w/w) were used to explore the optimal condition.…”

Section: Resultsmentioning

confidence: 99%

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

Jiang

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: ⊍-lactalbumin (⊍-La) is of great interest to the industry as a result of its excellent functional properties and nutritional value. Aqueous two-phase flotation (ATPF) of thermo-sensitive polymer poly (ethylene glycol-ran-propylene glycol) monobutyl ether (UCON) and KH 2 PO 4 was applied to directly separate and purify ⊍-La from milk whey, which was purposed to simplify the production process and reduced cost of production.RESULTS: The effect of ATPF composition and operating parameters on the flotation efficiency (E) and purity of ⊍-La were investigated. The optimal conditions included 2 min of premixing time, 30 mL min −1 flow velocity and 20 min of flotation time, whereas the composition conditions comprised 35.0 mL 0.18 g mL −1 phosphate solution (containing 10% (cow milk whey/salt solution, v/v) cow milk whey, 50 ppm defoamer and 2 g NaCl) and 5.0 mL of 40% (w/w) UCON solution. Under the optimal conditions, E of ⊍-La was 95.67 ± 1.04% and purity of ⊍-La was 98.78 ± 1.19%. UCON was recovered by a thermally-induced phase separation and reused in next ATPF process without reducing E of ⊍-La. Purified ⊍-La was characterized by several key technologies. The results indicated that ⊍-La in cow milk whey could be directly separated and purified by the ATPF and the purity was satisfactory. Moreover, it was suggested there was no obvious structure difference between the ⊍-La separated by ATPF and the ⊍-La standard.CONCLUSION: The present study enabled the recycling of UCON, providing an effective, economically viable and environmentally friendly approach for the separation and purification of protein.

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Aqueous two-phase systems with thermo-sensitive EOPO co-polymer (UCON) and sulfate salts: Effect of temperature and cation

Cited by 20 publications

References 21 publications

Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives

Bacteriocin partitioning from a clarified fermentation broth of Lactobacillus plantarum ST16Pa in aqueous two-phase systems with sodium sulfate and choline-based salts as additives

Extraction in cholinium-based magnetic ionic liquid aqueous two-phase system for the determination of berberine hydrochloride in Rhizoma coptidis

Direct separation and purification of α‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

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