Aqueous zymography screening of matrix metalloproteinase activity and inhibition based on colorimetric gold nanoparticles

Chuang, Yao-Chen; Huang, Wei; Chiang, Pin Hsuan; Tang, Meng Che; Lin, Chih‐Sheng

doi:10.1016/j.bios.2011.11.002

Cited by 25 publications

(6 citation statements)

References 50 publications

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“…The synthesized AuNPs‐oligonucleotide probes were characterized by a microplate spectrophotometer from 400 to 700 nm. A shift in absorbance peak confirmed whether the oligonucleotides were successfully attached to the surface of AuNPs to form AuNPs‐oligonucleotide probes or not (Chuang, Huang, Chiang, Tang, & Lin, 2012).…”

Section: Methodsmentioning

confidence: 83%

Using loop‐mediated isothermal amplification combined with gold nanoparticles for optically rapid detection of shrimp Vibrio parahaemolyticus

Sun

Lee

et al. 2022

Journal of Food Safety

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Shrimp is one of the most important aquatic products in the world. Early detection, prevention, and treatment of shrimp pathogens is important. One major shrimp pathogen, Vibrio parahaemolyticus causes acute hepatopancreatic necrosis disease (VPAHPND) has been studied, but research into pathogen detection needs to be strengthened. Therefore, we were targeting to VPAHPND and using emerging molecular biotechnologies to develop a method for pathogens detection for the shrimp industry. Gold nanoparticles (AuNPs) have an unique color change property. Free AuNPs in solution are the color of claret‐red, while the aggregated AuNPs appear slight purple. Based on the characteristics of visible color difference of AuNPs, we worked on establishing a simple, fast, and specific loop‐mediated isothermal amplification (LAMP) detection method for VPAHPND diagnosis using AuNPs. This method, amplified the target DNA of VPAHPND by LAMP and the DNA were directly added onto AuNPs solution for detection. The test results show a color change, so that the results can be visually inspected by naked eye. Using the methods, shrimp VPAHPND can be early detected and treated, boost the economic benefits, and efficiency of shrimp farming.

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Section: Methodsmentioning

confidence: 83%

Using loop‐mediated isothermal amplification combined with gold nanoparticles for optically rapid detection of shrimp Vibrio parahaemolyticus

Sun

Lee

et al. 2022

Journal of Food Safety

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“…Gel electrophoresis was performed to confirm the change in color of AuNPs after modification and enzyme digestion. 12 Visible color changes in AuNPs red band and UV light excited fluorescence band could be observed. Furthermore, 1.5% agarose gel was prepared with working TBE buffer (45 mM Tris-borate/1 mM EDTA).…”

Section: Methodsmentioning

confidence: 92%

“…AuNPs were prepared by citrate reduction method according to the reported protocol. 12 A 50 mL aqueous solution in a conical flask containing 1.0 mM chloroauric acid was heated to a vigorous boil with stirring. Then, 38.8 mM sodium citrate solution (5 mL) was rapidly added to the solution.…”

Section: Methodsmentioning

confidence: 99%

Detection of chymase activity using a specific peptide probe conjugated onto gold nanoparticles

et al. 2018

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Gold nanoparticles (AuNPs) can be applied in biosensors using fluorescence resonance energy transfer (FRET) technique. Based on this technique, we have established a sensitive and efficient biosensing method by modifying a peptide-probe onto AuNPs to detect proteinase enzyme activity in this study.This biosensing method was designed for chymase activity detection and applied in kidney disease diagnosis. In this study, 16 nm-AuNPs were used to construct the AuNPs-based fluorescence peptide probe (named AuNPs-peptide probe) for chymase activity determination. The peptide sequence is FITC-Acp-DRVYIHPFHLDDDDDC, which comprises a fluorophore at the N-terminal end, an enzyme (chymase) substrate (DRVYIHPFHL), a spacer (DDDDD) and cysteine (C) to conjugate to AuNPs surface. When the enzyme catalyzes the substrate sequence, the fluorophore drifts away from AuNPs and the fluorescence emitting signal can be excited at 495 nm and detected at 515 nm. The results indicate that the time required for the AuNPs-peptide probe for activity detection of chymase was only 15 min, and a linear correlation from 10 to 100 ng mL À1 of chymase was acquired. The chymase reaction would be significantly inhibited by addition of specific chymase inhibitor chymostatin. The AuNPs-peptide probe was tested for the detection of high concentrations of trypsin and chymotrypsin, but only minor emitted fluorescence intensity was detected. According to these results, sensitivity and specificity of the AuNPspeptide probe for chymase detection have been confirmed. AuNPs-peptide probe was successfully used for the detection of renal chymase activity; and the results indicate the pathogenically increased chymase activity in kidney tissue of nephropathic mice from aristolochic acid I treatment.

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“…), stability for a long period of time, easy surface functionalization, biocompatibility, unique optical properties and high catalytic activity provide the successful use of gold nanoparticles in the development of various bio-and nanosensors. [7][8][9][10][11] The specific characteristics mentioned above determine the main features of AuNP use in the development of different types of biosensors and nanosensors for detection of various samples including inorganic and organic pollutants, 4,12-14 toxins, 15,16 pathogens, 5,17 biomolecules, 6,[18][19][20] enzyme activity, 10,21 as well as diagnostics of different diseases, 22,23 etc. Thus, unique size-dependent optical properties of AuNP in combination with capabilities to surface functionalization define their effective use in the development of various LSPR-based, SERS-based and colorimetric nano-and biosensors.…”

Section: Discussionmentioning

confidence: 99%

Features of gold nanoparticles application for bio- and nanosensors

Рєзніченко¹

2018

IJBSBE

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Aqueous zymography screening of matrix metalloproteinase activity and inhibition based on colorimetric gold nanoparticles

Cited by 25 publications

References 50 publications

Using loop‐mediated isothermal amplification combined with gold nanoparticles for optically rapid detection of shrimp Vibrio parahaemolyticus

Using loop‐mediated isothermal amplification combined with gold nanoparticles for optically rapid detection of shrimp Vibrio parahaemolyticus

Detection of chymase activity using a specific peptide probe conjugated onto gold nanoparticles

Features of gold nanoparticles application for bio- and nanosensors

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