Aquimarina aggregata sp. nov., isolated from seawater

Abstract

A novel Gram-stain-negative, agarolytic, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated RZW4-3-2 T , was isolated from coastal seawater of the Yellow Sea in China (119.625 E 35.517 N). The organism grew optimally at 28 C, at pH 7.0 and in the presence of 2.0-3.0 % (w/v) NaCl. The strain required seawater or artificial seawater for growth, and NaCl alone did not support growth. Strain RZW4-3-2 T contained MK-6 as the major respiratory quinone and iso-C 17 : 0 3-OH, iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 15 : 0 3-OH as the dominant fatty acids. The polar lipids of strain RZW4-3-2 T comprised phosphatidylethanolamine, five unknown phospholipids and one unknown lipid. The DNA G+C content of strain RZW4-3-2 T was 32.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina pacifica SW150 T , Aquimarina macrocephali JCM 15542 T and Aquimarina muelleri LMG 22569 T with 98.1, 97.4 and 97.1 % sequence similarities, respectively. The estimated DNA-DNA hybridization values were 20.00±2.31 %, 20.70±2.33 % and 20.70±2.32 % between strain RZW4-3-2 T and A. pacifica SW150 T , A. macrocephali JCM 15542 T and A. muelleri LMG 22569 T , respectively. On the basis of results of polyphasic analyses, strain RZW4-3-2 T represents a novel species of the genus Aquimarina, for which the name Aquimarinaaggregata sp. nov. is proposed. The type strain is RZW4-3-2 T (=JCM 30824 T =MCCC 1K00698 T ).

Carapace microbiota in American lobsters (Homarus americanus) associated with epizootic shell disease and the green gland

“…The genus Aquimarina is a Gram-negative, aerobic, halophilic microorganism and affiliated with the phylum Bacteroidota (formerly Bacteroidetes ) and the family Flavobacteriaceae. They are normally found in marine environments ( Hahnke and Harder, 2013 ; Wang et al, 2016 , 2018 ), as well as in various marine hosts such as algae, sponges, and lobsters ( Kennedy et al, 2014 ; Kumar et al, 2016 ; Ranson et al, 2018 ). However, some Aquimarina species contain type 9 secretion system, gliding motility apparatus, and enzymes such as chitinase that breaks down crude chitin ( Hudson et al, 2019 ; Silva et al, 2019 ), which may contribute to development of ESD in lobsters.…”

Structural Insight into H‐NOX Gas Sensing and Cognate Signaling Protein Regulation

“…Until recently, it was thought that the glycine hinge was absolutely conserved, rendering this pivot point the universal hinge in H‐NOX proteins. However, continued genome searching revealed that several Flavobacteriaceae have H‐NOXs with native alanine substitutions in one of the glycine hinge residues (Figure B). These H‐NOXs provided an opportunity to directly interrogate the effect of steric bulk in the hinge region.…”

Comparative genomics reveals complex natural product biosynthesis capacities and carbon metabolism across host‐associated and free‐living Aquimarina (Bacteroidetes, Flavobacteriaceae) species

“…For instance, Aquimarina salinaria (Chen et al, 2012) was shown to possess antagonistic activity towards the toxic cyanobacterium Microcystis aeruginosa (Chen et al, 2011;Chen et al, 2012). Furthermore, at least three Aquimarina species have already been described to possess agarolytic activity, namely, A. aggregata, A. agarivorans and A. agarilytica (Lin et al, 2012;Zhou et al, 2015;Wang et al, 2016). Aquimarina species have as well been implicated in the emergence of epizootic shell disease (ESD) in the American lobster (Homarus americanus).…”