Arabica coffee accessions originated from Ethiopia with resistance to nematode Meloidogyne paranaensis

Abstract

The aim of this study was to evaluate the resistance of Ethiopian Coffea arabica from IAPAR’s germplasm bank to Meloidogyne paranaensis. Two experiments were carried out in a greenhouse in IAPAR, Londrina, Paraná State, Brazil. A completely randomized design was used with 15 treatments, eight replications and one plant per plot. 14 accessions of C. arabica from Ethiopia were evaluated and the cultivar Mundo Novo IAC 376-4 was used as a susceptible control. Seedlings with three to four pairs of leaves were transplanted into plastic cups with a capacity of 700 mL and 1200 eggs and/or J2 of M. paranaensis (IP) were inoculated after one month. The evaluations were performed 130 days after inoculation, when the data of the number of eggs and J2 per gram of roots, the final population of nematodes (FP) were obtained and the reproduction factor (RF) was calculated using the formula: RF = IP / FP. To classify the resistance levels of the genotypes, the reduction of the reproduction factor (RRF) was used. The Ethiopian accessions were classified from highly resistant to highly susceptible. Most of the Ethiopian coffees were highly resistant to M. paranaensis. The genotype Geisha also showed resistance, but is still in heterozygous condition.

Genome-wide association study for resistance to Pseudomonas syringae pv. garcae in Coffea arabica

“…Several studies, based on this C. arabica Ethiopian germplasm, identified sources of desirable traits, such as resistance to BHB ( Mohan et al., 1978 ); resistance to nematodes Meloidogyne paranaensis , M. incognita , and M. exigua ( Anzueto et al., 2001 ; Fatobene et al., 2017 ; Holderbaum et al., 2020 ); resistance to coffee berry disease ( van der Vossen and Walyaro, 2009 ); drought tolerance ( Queiroz-Voltan et al., 2014 ); biochemical compounds which influence beverage quality ( Bertrand et al., 2006 ); low caffeine content ( Silvarolla et al., 2004 ); and other chemical compounds ( Scholz et al., 2016 ).…”