Arabidopsis adc-silenced line exhibits differential defense responses to Botrytis cinerea and Pseudomonas syringae infection

Chávez-Martínez, Ana Isabel; Ortega-Amaro, María Azucena; Torres, M; Serrano, Mario; Jiménez-Bremont, Juan Francisco

doi:10.1016/j.plaphy.2020.09.035

Cited by 15 publications

(8 citation statements)

References 34 publications

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“…In our study, the mlo mutant displayed spontaneous cell death which may play a major role in plant susceptibility to S. sclerotiorum although PR1 expression was highly induced. In fact, some Arabidopsis mutants with higher PR1 expression but decreased resistance to necrotrophic pathogens were also reported like adc (arginine decarboxylase enzymes) mutants [ 68 ]. This was consistent with our results.…”

Section: Discussionmentioning

confidence: 99%

Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.

Liu

Zhang

et al. 2023

Biotechnol Biofuels

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Background Oilseed rape (Brassica napus L.) is known as one of the most important oilseed crops cultivated around the world. However, its production continuously faces a huge challenge of Sclerotinia stem rot (SSR), a destructive disease caused by the fungus Sclerotinia sclerotiorum, resulting in huge yield loss annually. The SSR resistance in B. napus is quantitative and controlled by a set of minor genes. Identification of these genes and pyramiding them into a variety are a major strategy for SSR resistance breeding in B. napus. Results Here, we performed a genome-wide association study (GWAS) using a natural population of B. napus consisting of 222 accessions to identify BnaA08g25340D (BnMLO2_2) as a candidate gene that regulates the SSR resistance. BnMLO2_2 was a member of seven homolog genes of Arabidopsis Mildew Locus O 2 (MLO2) and the significantly SNPs were mainly distributed in the promoter of BnMLO2_2, suggesting a role of BnMLO2_2 expression level in the regulation of SSR resistance. We expressed BnMLO2_2 in Arabidopsis and the transgenic plants displayed an enhanced SSR resistance. Transcriptome profiling of different tissues of B. napus revealed that BnMLO2_2 had the most expression level in leaf and silique tissues among all the 7 BnMLO2 members and also expressed higher in the SSR resistant accession than in the susceptible accession. In Arabidopsis, mlo2 plants displayed reduced resistance to SSR, whereas overexpression of MLO2 conferred plants an enhanced SSR resistance. Moreover, a higher expression level of MLO2 showed a stronger SSR resistance in the transgenic plants. The regulation of MLO2 in SSR resistance may be associated with the cell death. Collinearity and phylogenetic analysis revealed a large expansion of MLO family in Brassica crops. Conclusion Our study revealed an important role of BnMLO2 in the regulation of SSR resistance and provided a new gene candidate for future improvement of SSR resistance in B. napus and also new insights into understanding of MLO family evolution in Brassica crops.

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Section: Discussionmentioning

confidence: 99%

Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.

Liu

Zhang

et al. 2023

Biotechnol Biofuels

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“…Generally, reduced Put levels, in adc loss of function mutants and adc -silenced lines, result in pathogen susceptibility [ 183 , 195 ]. Sánchez-Rangel et al [ 51 ] generated a transgenic line of A. thaliana that silences ADC1 and ADC2 genes; this adc -silenced line is a non-lethal line with reduced ADC gene expression and low Put levels and high levels of ROS.…”

Section: Resistance Responses To Biotic Stressesmentioning

confidence: 99%

“…Sánchez-Rangel et al [ 51 ] generated a transgenic line of A. thaliana that silences ADC1 and ADC2 genes; this adc -silenced line is a non-lethal line with reduced ADC gene expression and low Put levels and high levels of ROS. Chávez-Martínez et al [ 195 ] examined the response of this adc -silenced line against two pathogens with different lifestyles. This silenced line was more susceptible to Botrytis cinerea , and the expression of PR1 was upregulated, while the jasmonic acid-related genes LOX3 and PDF1.2 and PAD3 involved in camalexin biosynthesis were downregulated.…”

Section: Resistance Responses To Biotic Stressesmentioning

confidence: 99%

Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress

González-Hernández

Scalschi

Vicedo

et al. 2022

IJMS

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Putrescine (Put) is the starting point of the polyamines (PAs) pathway and the most common PA in higher plants. It is synthesized by two main pathways (from ornithine and arginine), but recently a third pathway from citrulline was reported in sesame plants. There is strong evidence that Put may play a crucial role not only in plant growth and development but also in the tolerance responses to the major stresses affecting crop production. The main strategies to investigate the involvement of PA in plant systems are based on the application of competitive inhibitors, exogenous PAs treatments, and the most efficient approaches based on mutant and transgenic plants. Thus, in this article, the recent advances in understanding the role of this metabolite in plant growth promotion and protection against abiotic and biotic stresses will be discussed to provide an overview for future research.

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“…In most cases, Put levels in infected tissues are the result of de novo biosynthesis (Kim, Kim, & Hwang, 2013; Rossi, Marina, & Pieckenstain, 2015), although it has also been proposed that Put is excreted by the pathogen during plant tissue colonisation (Vilas et al, 2018). Reduced Put levels, in adc loss‐of‐function mutants and adc ‐silenced lines, result in pathogen susceptibility (Chávez‐Martínez, Ortega‐Amaro, Torres, Serrano, & Jiménez‐Bremont, 2020; Kim et al, 2013; Liu et al, 2020). Overexpression of the AtADC2 gene, as well as Put supplementation in Arabidopsis , elicit the expression of plant defence genes and stimulate local salicylic acid biosynthesis (Alcázar, García‐Martínez, Cuevas, Tiburcio, & Altabella, 2005; Liu et al, 2020).…”

Section: Pa Metabolism During Plant Responses To Pathogen Attackmentioning

confidence: 99%

Current status and perspectives on the role of polyamines in plant immunity

González

Jasso-Robles

Flores‐Hernández

et al. 2021

Annals of Applied Biology

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Polyamines are low molecular weight amines, with versatile and essential functions during the lifespan of prokaryotes and eukaryotes. Modulation of polyamine metabolism under biotic stress has attracted a great deal of interest; however, the precise functions of these amines in plant defence responses to pathogen attack are still poorly understood. Herein we summarise and review important aspects of the regulation of polyamine biosynthesis, catabolism and conjugation under biotic stress that might contribute to improve present‐day knowledge about these compounds in plant immunity. Particular attention is paid to the intracellular localisation of the proteins/enzymes associated to polyamine metabolism, as well as the impact of pathogen effector proteins on the control of plant polyamine metabolism is discussed.

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Arabidopsis adc-silenced line exhibits differential defense responses to Botrytis cinerea and Pseudomonas syringae infection

Cited by 15 publications

References 34 publications

Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.

Functional and evolutionary study of MLO gene family in the regulation of Sclerotinia stem rot resistance in Brassica napus L.

Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress

Current status and perspectives on the role of polyamines in plant immunity

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