Arabidopsis AtMSRB5 functions as a salt-stress protector for both Arabidopsis and rice

Abstract

Salinity, drought and low temperature are major environmental factors that adversely affect crop productivity worldwide. In this study we adopted an activation tagging approach to identify salt tolerant mutants of Arabidopsis. Thousands of tagged Arabidopsis lines were screened to obtain several potential mutant lines resistant to 150 mM NaCl. Transcript analysis of a salt-stress tolerance 1 (sst1) mutant line indicated activation of AtMSRB5 and AtMSRB6 which encode methionine sulfoxide reductases. Overexpression of AtMSRB5 in Arabidopsis (B5OX) showed a similar salt tolerant phenotype. Furthermore, biochemical analysis indicated stability of the membrane protein, H+-ATPase 2 (AHA2) through regulation of Na+/K+ homeostasis which may be involved in a stress tolerance mechanism. Similarly, overexpression of AtMSRB5 in transgenic rice demonstrated a salt tolerant phenotype via the modulation of Na+/K+ homeostasis without a yield drag under salt and oxidative stress conditions.

Genome-Wide Identification and Analysis of Plasma Membrane H+-ATPases Associated with Waterlogging in Prunus persica (L.) Batsch

“…AHA2 (AT4G30190) is involved in the low potassium response by forming a proton motive force in roots and by promoting solute uptake [62]. It is also involved in iron uptake [75] and salt stress [76,77]. In Group 3, there were two Arabidopsis genes, two peach genes, and three rice genes, among which that the (AHA4) AT3G47950 responded to drought treatment and could be involved in water use efficiency.…”