Arabidopsis CP12 mutants have reduced levels of phosphoribulokinase and impaired function of the Calvin–Benson cycle

López-Calcagno, Patricia E.; Abuzaid, Amani Omar; Lawson, Tracy; Raines, Christine A.

doi:10.1093/jxb/erx084

Cited by 51 publications

(51 citation statements)

References 70 publications

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“…S2), suggesting a less rigid structure. Since cyanobacterial and eukaryotic PRKs are found together with CP12, the greater flexibility of PRK could enable the formation of regulatory GAPDH-CP12-PRK complexes (9)(10)(11)(12)(13)(14)(15). Moreover, AtPRK and CrPRK are characterized by an increased number of exposed random-coiled regions ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin–Benson cycle

Gurrieri

Giudice

Demitri

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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In land plants and algae, the Calvin–Benson (CB) cycle takes place in the chloroplast, a specialized organelle in which photosynthesis occurs. Thioredoxins (TRXs) are small ubiquitous proteins, known to harmonize the two stages of photosynthesis through a thiol-based mechanism. Among the 11 enzymes of the CB cycle, the TRX target phosphoribulokinase (PRK) has yet to be characterized at the atomic scale. To accomplish this goal, we determined the crystal structures of PRK from two model species: the green alga Chlamydomonas reinhardtii (CrPRK) and the land plant Arabidopsis thaliana (AtPRK). PRK is an elongated homodimer characterized by a large central β-sheet of 18 strands, extending between two catalytic sites positioned at its edges. The electrostatic surface potential of the catalytic cavity has both a positive region suitable for binding the phosphate groups of substrates and an exposed negative region to attract positively charged TRX-f. In the catalytic cavity, the regulatory cysteines are 13 Å apart and connected by a flexible region exclusive to photosynthetic eukaryotes—the clamp loop—which is believed to be essential for oxidation-induced structural rearrangements. Structural comparisons with prokaryotic and evolutionarily older PRKs revealed that both AtPRK and CrPRK have a strongly reduced dimer interface and an increased number of random-coiled regions, suggesting that a general loss in structural rigidity correlates with gains in TRX sensitivity during the molecular evolution of PRKs in eukaryotes.

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Section: Resultsmentioning

confidence: 99%

“…S1). In addition, PRK forms a regulatory complex with the redox-sensitive CP12 and the photosynthetic GAPDH (A 4 -GAPDH and AB-GAPDH) (9)(10)(11)(12)(13)(14)(15). The formation of this ternary complex occurs in both plants and algae, and causes an almost complete inhibition of PRK activity (11,16).…”

mentioning

confidence: 99%

Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin–Benson cycle

Gurrieri

Giudice

Demitri

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Up-regulation of SgCP12 expression resulted in promoted plant growth with increased plant height and biomass, while down-regulation led to reduced plant height and biomass. The phenotype in antisense S. guianensis are similar to CP12 antisense transgenic tobacco and Arabidopsis double or triple mutants cp12-1 / 2 and cp12-1 / 2 / 3 showing a reduced growth phenotype ( Howard et al, 2011 ; López-Calcagno et al, 2017 ). It is suggested that SgCP12 is a good candidate gene used for improving plant growth and chilling tolerance.…”

Section: Discussionmentioning

confidence: 71%

“…PRK and GAPDH activities were lower in antisense plants than in the wild type under both control and chilling conditions. Likely, reduced PRK protein levels were observed in Arabidopsis mutants lacking CP12-1 transcript, but Rubisco and GAPDH protein levels were not altered ( López-Calcagno et al, 2017 ). There was a slight reduction in GAPDH and PRK activities in CP12 antisense tobacco plants although it is not statistically different ( Howard et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…Carbon allocation to the cell wall and organic acids is increased and carbon going to starch and soluble carbohydrates is decreased in CP12 antisense plants which showed a phenotype of dwarfism and reduced apical dominance ( Howard et al, 2011 ). No severe photosynthetic or growth phenotype was observed in Arabidopsis single cp12-1, cp12-2 , and cp12-3 mutants, but reduced transcripts of both CP12-1 and CP12-2 lead to reduced PRK protein level and photosynthetic capacity and a phenotype with slower growth and reduced seed yield in double or triple mutants CP12-1 / 2 and CP12-1 / 2 / 3 ( López-Calcagno et al, 2017 ). However, it is unknown what phenotypes display in transgenic plants overexpressing CP12 .…”

Section: Introductionmentioning

confidence: 99%

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Chloroplast Protein 12 Expression Alters Growth and Chilling Tolerance in Tropical Forage Stylosanthes guianensis (Aublet) Sw

Qiu

Zhou

et al. 2018

Front. Plant Sci.

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Stylosanthes guianensis (Aublet) Sw. is a tropical forage legume with soil acidity tolerance and excellent adaptation to infertile soils, but sensitive to chilling. To understand the molecular responses of S. guianensis to chilling, differentially expressed genes between a chilling tolerant mutant 7–1 and the wild type were identified using suppression subtractive hybridization, and eight of them were confirmed and the regulation pattern were analyzed using quantitative reverse transcription PCR (qRT-PCR). Chloroplast protein 12 (CP12) functions to regulate the Calvin cycle by forming a ternary complex with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK). SgCP12 transcript was induced by chilling in both plants, and higher levels were observed in 7–1 than in the wild type, implying a potential role of SgCP12 in chilling tolerance. To confirm this, transgenic S. guianensis plants over-expressing or down-regulating SgCP12 were generated, respectively. Higher Fv/Fm and survival rate and lower ion leakage were observed in transgenic plants overexpressing SgCP12 as compared with the wild type after chilling treatment, while lower Fv/Fm and survival rate and higher ion leakage were found in SgCP12 antisense plants. SgCP12 overexpression plants showed promoted growth with increased plant height and fresh weight, while the antisense plants exhibited reduced growth with decreased plant height and fresh weight as compared with the wild type. The results indicated that regulation of SgCP12 expression alters plant growth and chilling tolerance in S. guianensis. In addition, higher levels of net photosynthetic rate (Pn), GAPDH and PRK activities were observed in SgCP12 overexpression transgenic plants, while lower levels in antisense plants than in the wild type under both control and chilling conditions, indicating that altered activities of GAPDH and PRK were associated with the changed Pn in transgenic S. guianensis. Our results suggest that SgCP12 regulates GAPDH and PRK activities, Pn, and chilling tolerance in S. guianensis.

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CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production

Barupal,

Kumar

2024

Gene Editing in Plants

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Arabidopsis CP12 mutants have reduced levels of phosphoribulokinase and impaired function of the Calvin–Benson cycle

Abstract: HighlightReverse genetics approaches revealed functional redundancy between the CP12-1 and CP12-2 proteins and a role for these proteins in determining the level of the Calvin–Benson cycle enzyme phosphoribulokinase.

Cited by 51 publications

References 70 publications

Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin–Benson cycle

Arabidopsis and Chlamydomonas phosphoribulokinase crystal structures complete the redox structural proteome of the Calvin–Benson cycle

Chloroplast Protein 12 Expression Alters Growth and Chilling Tolerance in Tropical Forage Stylosanthes guianensis (Aublet) Sw

CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production

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