Arabidopsis CPR5 plays a role in regulating nucleocytoplasmic transport of mRNAs in ethylene signaling pathway

Abstract

The ETR1 receptor plays a predominant role in ethylene signaling in Arabidopsis thaliana. Previous studies showed that both RTE1 and CPR5 can directly bind to the ETR1 receptor and regulate ethylene signaling. RTE1 was suggested to promote the ETR1 receptor signaling by in uencing its conformation, but little is known about the regulatory mechanism of CPR5 in ethylene signaling. In this study, we presented data showing that both RTE1 and CPR5 bound to the N-terminal domains of ETR1, and regulated ethylene signaling via the ethylene receptor. On the other hand, the research provided evidence indicating that CPR5 could act as a nucleoporin to regulate the ethylene-related mRNAs export out of the nucleus, while RTE1 or its homolog (RTH) had no effect on the nucleocytoplasmic transport of mRNAs.Nuclear qRT-PCR analysis and poly(A)-mRNA in situ hybridization showed that defect of CPR5 restricted nucleocytoplasmic transport of mRNAs. These results advance our understanding of the regulatory mechanism of CPR5 in ethylene signaling. Key MessageArabidopsis CPR5 is involved in regulation of ethylene signaling via two different ways: interacting with the ETR1 N-terminal domains, and controlling nucleocytoplasmic transport of ethylene-related mRNAs.

Novel Bisexual Flower Control Gene Regulates Sex Differentiation in Melon (Cucumis melo L.)

“…Ethylene plays a key role in melon sex determination through signal transduction processes. CPR5 has been proven to be a key gene in the ethylene signal transduction pathway, where it promotes ethylene signal transduction by directly binding to ETR1 (ethylene receptor protein). , We hypothesized that the ethylene signaling pathway mediated by CmCPR5 mutation in Y101 was dysfunctional. Since using ethephon solution is a common method of investigating the response of sex-determination-related genes to ethylene in Cucumis, ,− we investigated the expression pattern of CmCPR5 under different ethephon treatments, according to previous studies. , …”

“…4 was found to encode CPR5 protein, which is a multipotent enzyme involved in many plant growth and development processes and disease regulation pathways. Notably, CPR5 responds to ethylene, and recently, AtCPR5 has been shown to regulate ethylene signaling in A. thaliana by interacting with the ethylene receptor AtETR1 . , Based on this, we analyzed the expression of CmCPR5 (Candidate No. 4, MELO3C007802) and found that the expression was suppressed in the bisexual line Y101 but induced in the unisexual line 0426 by ethylene in a dose-dependent manner.…”

“…Presently, five ethylene receptors (ETR1/2, ERS1/2, and EIN4) are involved in ethylene signal transduction, among which ETR1 is the predominant one. − ETR1 is regulated by other components of the ethylene signal transduction pathway, including RTE1 (Reversion to Ethylene Sensitivity 1), a positive upstream regulator of ETR1 which is plant specific. − A novel positive upstream regulator of ETR1, AtCPR5 has been recently identified in Arabidopsis thaliana where it interacts with AtETR1 to regulate ethylene responses and signaling . Additionally, Chen demonstrated that Arabidopsis CPR5 binds to the N-terminal domains of ETR1 to regulate ethylene signaling via ethylene receptors …”

“…34 Additionally, Chen demonstrated that Arabidopsis CPR5 binds to the N-terminal domains of ETR1 to regulate ethylene signaling via ethylene receptors. 35 Melons exhibit diverse sexual types associated with complex determination mechanisms, which have been widely studied for many years. The andromonoecious type has been the main target in melon breeding; however, the development mechanisms of unisexual/bisexual flowers in melons have also been explored.…”

Genome-Wide Identification and Characterization of CPR5 Genes in Gossypium Reveals Their Potential Role in Trichome Development

“…The involvement of CPR5 with plant disease defense had been deeply studied in Arabidopsis . The study showed that CPR5 played important roles in signaling pathways, such as CKI-RB-E2F cell cycle signaling pathway, activated immune responses, and ROS signal transduction ( Borghi et al, 2011 ; Chen et al, 2022 ). CPR5 was also found to be involved with multiple plant hormones signaling pathways, such as salicylic acid and ethylene ( Clarke et al, 2004 ).…”