Arabidopsis G‐protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Klopffleisch, Karsten; Phan, Nguyen; Augustin, Kelsey; Bayne, Robert S.; Booker, Katherine S.; Botella, José Ramón; Carpita, Nicholas C.; Carr, Tyrell; Chen, Jay; Cooke, Thomas Ryan; Frick-Cheng, Arwen E; Friedman, Erin J.; Fulk, Brandon; Hahn, Michael G.; Jiang, Kun; Jordá, Lucía; Kruppe, Lydia Linnéa; Liu, Chenggang; Lorek, Justine; McCann, Maureen C.; Molina, Antonio; Moriyama, Etsuko N.; Mukhtar, M. Shahid; Mudgil, Yashwanti; Pattathil, Sivakumar; Schwarz, John J.; Seta, Steven; Tan, Matthew; Temp, Ulrike; Trusov, Yuri; Urano, Daisuke; Welter, Bastian; Yang, Jing; Panstruga, Ralph; Uhrig, Joachim F.; Jones, Alan M.

doi:10.1038/msb.2011.66

Cited by 192 publications

(189 citation statements)

References 44 publications

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“…When agb1 and agg1 agg2 mutants were inoculated with P. syringae pv tomato DC3118 by spraying, they supported a small but significant increase of P. syringae pv tomato DC3118 growth compared with the wild type, suggesting that stomatal defense could be affected in these mutants as well. In agb1 mutants, stomatal density was shown to be higher than in the wild type (Zhang et al, 2008a;Klopffleisch et al, 2011). The increased stomatal density is probably also a contributing factor to higher P. syringae pv tomato DC3118 growth in the agb1 mutants.…”

Section: Discussionmentioning

confidence: 92%

“…Similar to AGB1, ER is also required for resistance against P. cucumerina (Llorente et al, 2005). Recently, both er and agb1 mutants were found to have altered cell wall structure (Sánchez-Rodríguez et al, 2009;Klopffleisch et al, 2011;Delgado-Cerezo et al, 2012). The similarity of the mutant phenotypes in er and agb1 mutants suggests that AGB1 may be required for the functions of ER in the regulation of development, cell wall structure, and resistance against P. cucumerina.…”

Section: Discussionmentioning

confidence: 95%

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Heterotrimeric G Proteins Serve as a Converging Point in Plant Defense Signaling Activated by Multiple Receptor-Like Kinases

et al. 2013

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In fungi and metazoans, extracellular signals are often perceived by G-protein-coupled receptors (GPCRs) and transduced through heterotrimeric G-protein complexes to downstream targets. Plant heterotrimeric G proteins are also involved in diverse biological processes, but little is known about their upstream receptors. Moreover, the presence of bona fide GPCRs in plants is yet to be established. In Arabidopsis (Arabidopsis thaliana), heterotrimeric G protein consists of one Gα subunit (G PROTEIN α-SUBUNIT1), one Gβ subunit (ARABIDOPSIS G PROTEIN β-SUBUNIT1 [AGB1]), and three Gγs subunits (ARABIDOPSIS G PROTEIN γ-SUBUNIT1 [AGG1], AGG2, and AGG3). We identified AGB1 from a suppressor screen of BAK1-interacting receptor-like kinase1-1 (bir1-1), a mutant that activates cell death and defense responses mediated by the receptor-like kinase (RLK) SUPPRESSOR OF BIR1-1. Mutations in AGB1 suppress the cell death and defense responses in bir1-1 and transgenic plants overexpressing SUPPRESSOR OF BIR1-1. In addition, agb1 mutant plants were severely compromised in immunity mediated by three other RLKs, FLAGELLIN-SENSITIVE2 (FLS2), Elongation Factor-TU RECEPTOR (EFR), and CHITIN ELICITOR RECEPTOR KINASE1 (CERK1), respectively. By contrast, G PROTEIN α-SUBUNIT1 is not required for either cell death in bir1-1 or pathogen-associated molecular pattern-triggered immunity mediated by FLS2, EFR, and CERK1. Further analysis of agg1 and agg2 mutant plants indicates that AGG1 and AGG2 are also required for pathogen-associated molecular pattern-triggered immune responses mediated by FLS2, EFR, and CERK1, as well as cell death and defense responses in bir1-1. We hypothesize that the Arabidopsis heterotrimeric G proteins function as a converging point of plant defense signaling by mediating responses initiated by multiple RLKs, which may fulfill equivalent roles to GPCRs in fungi and animals.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Heterotrimeric G Proteins Serve as a Converging Point in Plant Defense Signaling Activated by Multiple Receptor-Like Kinases

et al. 2013

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“…These observations indicate a defect in cell division and elongation as the possible cause of gpa1 morphological phenotypes, which also could explain the short hypocotyl and open apical hook of gpa1 etiolated seedlings (Lease et al, 2001;Ullah et al, 2002Ullah et al, , 2003. Interestingly, the assembly of a G-protein interactome has revealed that GPA1 interacts with proteins that are involved in the modulation of cell wall composition or structure (Klopffleisch et al, 2011), like those we reported misregulated in the adt3 proteome (Table I), providing a possible link between G-proteins and morphogenesis. ADT3 is part of a signaling module that includes GPA1 and GCR1, whose activation by blue light leads to Phe synthesis (Warpeha et al, 2006(Warpeha et al, , 2008.…”

Section: Adt3 In Signaling and Developmentmentioning

confidence: 98%

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

et al. 2016

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During the transition from seed to seedling, emerging embryos strategically balance available resources between building up defenses against environmental threats and initiating the developmental program that promotes the switch to autotrophy. We present evidence of a critical role for the phenylalanine (Phe) biosynthetic activity of AROGENATE DEHYDRATASE3 (ADT3) in coordinating reactive oxygen species (ROS) homeostasis and cotyledon development in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. We show that ADT3 is expressed in the cotyledon and shoot apical meristem, mainly in the cytosol, and that the epidermis of adt3 cotyledons contains higher levels of ROS. Genome-wide proteomics of the adt3 mutant revealed a general down-regulation of plastidic proteins and ROS-scavenging enzymes, corroborating the hypothesis that the ADT3 supply of Phe is required to control ROS concentration and distribution to protect cellular components. In addition, loss of ADT3 disrupts cotyledon epidermal patterning by affecting the number and expansion of pavement cells and stomata cell fate specification; we also observed severe alterations in mesophyll cells, which lack oil bodies and normal plastids. Interestingly, up-regulation of the pathway leading to cuticle production is accompanied by an abnormal cuticle structure and/or deposition in the adt3 mutant. Such impairment results in an increase in cell permeability and provides a link to understand the cell defects in the adt3 cotyledon epidermis. We suggest an additional role of Phe in supplying nutrients to the young seedling.

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“…Experimental PPIs of CORNET consist of data retrieved from BIND (Bader et al, 2003), IntAct (Hermjakob et al, 2004), BioGRID (Stark et al, 2006), DIP (Salwinski et al, 2004), MINT (Chatr-aryamontri et al, 2007), TAIR (Rhee et al, 2003), Arabidopsis Interactome Mapping (Arabidopsis Interactome Mapping Consortium, 2011), MIND 0.5 (Lalonde et al, 2010), and the G-protein interactome (Klopffleisch et al, 2011). Predicted PPIs were not considered in this study.…”

Section: Cornet 20 Datamentioning

confidence: 99%

The Potential of Text Mining in Data Integration and Network Biology for Plant Research: A Case Study onArabidopsis

et al. 2013

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Despite the availability of various data repositories for plant research, a wealth of information currently remains hidden within the biomolecular literature. Text mining provides the necessary means to retrieve these data through automated processing of texts. However, only recently has advanced text mining methodology been implemented with sufficient computational power to process texts at a large scale. In this study, we assess the potential of large-scale text mining for plant biology research in general and for network biology in particular using a state-of-the-art text mining system applied to all PubMed abstracts and PubMed Central full texts. We present extensive evaluation of the textual data for Arabidopsis thaliana, assessing the overall accuracy of this new resource for usage in plant network analyses. Furthermore, we combine text mining information with both protein-protein and regulatory interactions from experimental databases. Clusters of tightly connected genes are delineated from the resulting network, illustrating how such an integrative approach is essential to grasp the current knowledge available for Arabidopsis and to uncover gene information through guilt by association. All largescale data sets, as well as the manually curated textual data, are made publicly available, hereby stimulating the application of text mining data in future plant biology studies.

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Arabidopsis G‐protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Abstract: Yeast two-hybrid technology is used to build a high-quality protein interaction network centered on Arabidopsis G-protein coupled signaling. The interactions uncovered are without precedent in animals and fungi and help identify new cellular roles for G-protein signaling in plants.

Cited by 192 publications

References 44 publications

Heterotrimeric G Proteins Serve as a Converging Point in Plant Defense Signaling Activated by Multiple Receptor-Like Kinases

Heterotrimeric G Proteins Serve as a Converging Point in Plant Defense Signaling Activated by Multiple Receptor-Like Kinases

The dehydratase ADT3 affects ROS homeostasis and cotyledon development

The Potential of Text Mining in Data Integration and Network Biology for Plant Research: A Case Study onArabidopsis

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