Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism

Kansup, Jeeraporn; Tsugama, Daisuke; Liu, Shenkui; Takano, Tetsuo

doi:10.1016/j.bbrc.2014.01.106

Cited by 20 publications

(15 citation statements)

References 23 publications

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“…A GST pull‐down assay was conducted as described in [29]. Briefly, crude extract of GST‐AtTrx m2 fusion protein was bound to Glutathione Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis mitochondrial voltage‐dependent anion channel 3 (AtVDAC3) protein interacts with thioredoxin m2

et al. 2015

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Edited by Ulf-Ingo Flügge Keywords:Mitochondrial voltage-dependent anion channel Chloroplast thioredoxin Protein interaction NaCl stress a b s t r a c t Voltage-dependent anion channels (VDACs) are conserved mitochondrial outer membrane proteins. A yeast two-hybrid screen identified interaction between Arabidopsis VDAC3 and the chloroplast protein thioredoxin m2 (AtTrx m2). This was confirmed via pull-down assay. A bimolecular fluorescence complementation assay located the interaction in mitochondria. AtVDAC3 and AtTrx m2 transcripts were expressed in multiple tissues and up-regulated by abiotic stress. Under NaCl stress, AtVDAC3 overexpression inhibited growth and increased H 2 O 2 accumulation, while AtTrx m2 overexpression conferred resistance to NaCl and reduced H 2 O 2 . Results indicate that both AtVDAC3 and AtTrx m2 are involved in ROS signaling and play opposite roles in NaCl stress response.

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Section: Methodsmentioning

confidence: 99%

Arabidopsis mitochondrial voltage‐dependent anion channel 3 (AtVDAC3) protein interacts with thioredoxin m2

et al. 2015

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“…To identify AGB1-interacting proteins, we used AGB1 as a bait protein for a yeast two-hybrid screen of a normalized Arabidopsis cDNA library (for details, see Methods). The screening identified two potential AGB1 interactors: NPH3, a regulator of hypocotyl phototropism (Kansup et al, 2014), and BBX21 (GenBank: AAO42831; AT1G75540), a BBX-containing transcription factor. Because BBX21 is known to play a vital role in hypocotyl elongation (Datta et al, 2007;Xu et al, 2016), we selected BBX21 for further analysis.…”

Section: The Coiled-coil Domain Of Agb1 Physically Interacts With Thementioning

confidence: 99%

“…Hypocotyl development in Arabidopsis thaliana is a delicate developmental process regulated through the complex interplay between light and endogenous hormonal cues (Neff et al, 2006;Alabadí and Blá zquez, 2009;Lau and Deng, 2010;Boron and Vissenberg, 2014). Two mutants in AGB1 (agb1-1 and agb1-2) have a short hypocotyl phenotype and display decreased hypocotyl phototropism, relative to wild-type (Ullah et al, 2003;Chen et al, 2004;Kansup et al, 2014). In contrast, gpa1-4 (GPA1) has a slightly short hypocotyl phenotype, and gcr1-2 (GCR1, G-Protein-Coupled Receptor 1) hypocotyls develop normally compared with wild-type (Chen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, gpa1-4 (GPA1) has a slightly short hypocotyl phenotype, and gcr1-2 (GCR1, G-Protein-Coupled Receptor 1) hypocotyls develop normally compared with wild-type (Chen et al, 2004). Meanwhile, AGB1 has been shown to interact with NONPHOTOTROPIC HYPOCOTYL3 (NPH3), a regulator of phototropism that interacts with the blue light receptor, PHOT1 (Motchoulski and Liscum, 1999;Lariguet et al, 2006;Kansup et al, 2014), suggesting that AGB1 plays a pivotal role in regulating hypocotyl development. Studies have precluded a direct involvement of AGB1 and GPA1 in coupling phytochrome-mediated seedling photomorphogenesis, and the developmental defects observed in hypocotyls of G-protein mutants were attributed to altered hormone responses (Jones et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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The G-Protein β Subunit AGB1 Promotes Hypocotyl Elongation through Inhibiting Transcription Activation Function of BBX21 in Arabidopsis

Gao

et al. 2017

Molecular Plant

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Hypocotyl development in Arabidopsis thaliana is regulated by light and endogenous hormonal cues, making it an ideal model to study the interplay between light and endogenous growth regulators. BBX21, a B-box (BBX)-like zinc-finger transcription factor, integrates light and abscisic acid signals to regulate hypocotyl elongation in Arabidopsis. Heterotrimeric G-proteins are pivotal regulators of plant development. The short hypocotyl phenotype of the G-protein β-subunit (AGB1) mutant (agb1-2) has been previously identified, but the precise role of AGB1 in hypocotyl elongation remains enigmatic. Here, we show that AGB1 directly interacts with BBX21, and the short hypocotyl phenotype of agb1-2 is partially suppressed in agb1-2bbx21-1 double mutant. BBX21 functions in the downstream of AGB1 and overexpression of BBX21 in agb1-2 causes a more pronounced reduction in hypocotyl length, indicating that AGB1 plays an oppositional role in relation to BBX21 during hypocotyl development. Furthermore, we demonstrate that the C-terminal region of BBX21 is important for both its intracellular localization and its transcriptional activation activity that is inhibited by interaction with AGB1. ChIP assays showed that BBX21 specifically associates with its own promoter and with those of BBX22, HY5, and GA2ox1. which is not altered in agb1-2. These data suggest that the AGB1-BBX21 interaction only affects the transcriptional activation activity of BBX21 but has no effect on its DNA binding ability. Taken together, our data demonstrate that AGB1 positively promotes hypocotyl elongation through repressing BBX21 activity.

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“…To date, Y2/3H screening has been the primary method used to identify AGB1 interactors 125 (Mudgil et al, 2009;Kobayashi et al, 2012;Tsugama et al, 2012;Kansup et al, 2013;126 Tsugama et al, 2013;Kansup et al, 2014;Xu et al, 2015). In order to identify AGB1-associated 127 proteins in planta, we performed co-IP from transgenic Arabidopsis expressing 35S driven 128 FLAG-tagged AGB1 in the agb1-2 background, or from wild-type plants as a negative control.…”

Section: Identification Of Agb1-associated Proteins By Co-ip and Massmentioning

confidence: 99%

The G Protein β-Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement

Chakravorty

Assmann

2018

Plant Physiol.

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Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism

Cited by 20 publications

References 23 publications

Arabidopsis mitochondrial voltage‐dependent anion channel 3 (AtVDAC3) protein interacts with thioredoxin m2

Arabidopsis mitochondrial voltage‐dependent anion channel 3 (AtVDAC3) protein interacts with thioredoxin m2

The G-Protein β Subunit AGB1 Promotes Hypocotyl Elongation through Inhibiting Transcription Activation Function of BBX21 in Arabidopsis

The G Protein β-Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement

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