Arabidopsis Lectin Receptor Kinase P2K2 Is a Second Plant Receptor for Extracellular ATP and Contributes to Innate Immunity

Pham, An T.; Cho, Sung Hwan; Stacey, Gary

doi:10.1104/pp.19.01265

Cited by 87 publications

(106 citation statements)

References 58 publications

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“…It is the founding member of purinoreceptor (P2K) family, with DORN1 as P2K1 (Choi et al ., 2014). Very recently, Arabidopsis lectin receptor kinase LecRK‐I.5 was shown to act as a second plant receptor for eATP and designated as P2K2 (Pham et al ., 2020). P2K2 shares 74% amino acid similarity to P2K1 and binds to ATP with similar high affinity ( Kd = 44.47 ± 15.73 n m ).…”

Section: Apoplastic Dampsmentioning

confidence: 99%

Short‐ and long‐distance signaling in plant defense

Sun

Zhang²

2020

The Plant Journal

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Summary When encountering microbial pathogens, plant cells can recognize danger signals derived from pathogens, activate plant immune responses and generate cell‐autonomous as well as non‐cell‐autonomous defense signaling molecules, which promotes defense responses at the infection site and in the neighboring cells. Meanwhile, local damages can result in the release of immunogenic signals including damage‐associated molecule patterns and phytocytokines, which also serve as danger signals to potentiate immune responses in cells surrounding the infection site. Activation of local defense responses further induces the production of long‐distance defense signals, which can move to distal tissue to activate systemic acquired resistance. In this review, we summarize current knowledge on various signaling molecules involved in short‐ and long‐distance defense signaling, and emphasize the roles of regulatory proteins involved in the processes.

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Section: Apoplastic Dampsmentioning

confidence: 99%

Short‐ and long‐distance signaling in plant defense

Sun

Zhang²

2020

The Plant Journal

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“…In Arabidopsis thaliana , eATP can act as a damage-associated molecular pattern (DAMP) and activates immunity signalling through the plasma membrane purinoreceptor AtDORN1 (Does Not Respond to Nucleotides1, also known as P2K1) [ 7 ]. A plasma membrane co-receptor P2K2 has recently been identified and both DORN1/P2K1 and P2K2 are lectin receptor kinases [ 8 ]. eATP-dependent but AtDORN1-independent effects have also been reported [ 9 , 10 ], pointing to the presence of other perception mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots

Mohammad-Sidik

Sun

Shin

et al. 2021

IJMS

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Extracellular ATP (eATP) has long been established in animals as an important signalling molecule but this is less understood in plants. The identification of Arabidopsis thaliana DORN1 (Does Not Respond to Nucleotides) as the first plant eATP receptor has shown that it is fundamental to the elevation of cytosolic free Ca2+ ([Ca2+]cyt) as a possible second messenger. eATP causes other downstream responses such as increase in reactive oxygen species (ROS) and nitric oxide, plus changes in gene expression. The plasma membrane Ca2+ influx channels involved in eATP-induced [Ca2+]cyt increase remain unknown at the genetic level. Arabidopsis thaliana Annexin 1 has been found to mediate ROS-activated Ca2+ influx in root epidermis, consistent with its operating as a transport pathway. In this study, the loss of function Annexin 1 mutant was found to have impaired [Ca2+]cyt elevation in roots in response to eATP or eADP. Additionally, this annexin was implicated in modulating eATP-induced intracellular ROS accumulation in roots as well as expression of eATP-responsive genes.

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“…Previous research clearly indicates that eATP is an important stress signaling compound in plants, which in Arabidopsis is recognized by the P2K1 receptor, perhaps in combination with the recently identi ed, closely related P2K2 receptor 34 . Details of importance of eATP signaling in plants are emerging, including evidence that P2K1 contributes to plant defense against pathogens 33,48,49 , ATP mediated ROS signaling, JA levels and S-acylation 33,37,49 .…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial inoculation assay. The bacterial inoculation assay was performed with minor modi cations as previously described 34 . 2-week-old Arabidopsis seedlings were inoculated in 50 ml of Pseudomonas syringae pv.…”

Section: Methodsmentioning

confidence: 99%

“…The rst eATP receptor in plants, P2K1 (also termed DORN1 or LecRK-I.9) binds to ATP and ADP, leading to a variety of cellular changes, among them an increase in cytoplasmic Ca 2+ levels, production of reactive oxygen species (ROS), and defense-related transcriptional responses [30][31][32][33] . Recent studies have begun to unravel the complex signaling events that mediate purinergic signaling in plants but progress is still at an early stage 34 . In order to further elucidate the details of the plant eATP signaling pathway, we performed a forward genetic screen based on the aequorin-bioluminescence Ca 2+ imaging approach 35 .…”

Section: Introductionmentioning

confidence: 99%

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Activation of the plant mevalonate pathway by extracellular ATP

Cho¹,

Tóth²,

Kim³

et al. 2020

Preprint

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The mevalonate (MVA) pathway plays a critical role in multiple cellular processes in both animals and plants. In plants, the products of this pathway impact growth and development, as well as the response to environmental stress. A forward genetic screen of Arabidopsis thaliana using Ca2+ imaging identified mevalonate kinase (MVK) as a critical component of plant purinergic signaling. MVK interacts directly with the plant extracellular ATP (eATP) receptor P2K1 and is phosphorylated by P2K1 in response to eATP. Mutation of P2K1-mediated phosphorylation sites in MVK eliminates the ATP-induced cytoplasmic calcium response, MVK enzymatic activity, and suppresses pathogen defense. The data demonstrate that the plasma membrane associated P2K1 directly impacts plant cellular metabolism by phosphorylation of MVK, a key enzyme in the mevalonate pathway. The results underline the importance of purinergic signaling in plants and the ability of eATP to influence the activity of a key metabolite pathway with global effects on plant metabolism.

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Arabidopsis Lectin Receptor Kinase P2K2 Is a Second Plant Receptor for Extracellular ATP and Contributes to Innate Immunity

Cited by 87 publications

References 58 publications

Short‐ and long‐distance signaling in plant defense

Short‐ and long‐distance signaling in plant defense

Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots

Activation of the plant mevalonate pathway by extracellular ATP

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