Arabidopsis peroxidase AtPRX53 influences cell elongation and susceptibility to<i>Heterodera schachtii</i>

Jin, Jing; Hewezi, Tarek; Baum, Thomas J.

doi:10.4161/psb.6.11.17684

Cited by 35 publications

(36 citation statements)

References 71 publications

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“…Our findings are in agreement with previous reports describing other Arabidopsis CIII Prxs having negative effects on growth. For instance, overexpression of AtPRX37 causes dwarfism (Pedreira et al, 2011), and plants mutated for or overexpressing AtPRX53 exhibit longer or shorter hypocotyls, respectively (Jin et al, 2011). We also observed enhanced hypocotyl elongation in atprx71-1 and atprx71-2 etiolated seedlings; this latter result indicates that AtPRX71 regulates plant growth by restricting cell size rather than cell number, because it has been shown that elongation of etiolated hypocotyls occurs mainly by cell expansion (Gendreau et al, 1997).…”

Section: Discussionsupporting

confidence: 64%

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

et al. 2015

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The structure of the cell wall has a major impact on plant growth and development, and alteration of cell wall structural components is often detrimental to biomass production. However, the molecular mechanisms responsible for these negative effects are largely unknown. Arabidopsis (Arabidopsis thaliana) plants with altered pectin composition because of either the expression of the Aspergillus niger polygalacturonase II (AnPGII; 35S:AnPGII plants) or a mutation in the QUASIMODO2 (QUA2) gene that encodes a putative pectin methyltransferase (qua2-1 plants), display severe growth defects. Here, we show that expression of Arabidopsis PEROXIDASE71 (AtPRX71), encoding a class III peroxidase, strongly increases in 35S:AnPGII and qua2-1 plants as well as in response to treatments with the cellulose synthase inhibitor isoxaben, which also impairs cell wall integrity. Analysis of atprx71 loss-of-function mutants and plants overexpressing AtPRX71 indicates that this gene negatively influences Arabidopsis growth at different stages of development, likely limiting cell expansion. The atprx71-1 mutation partially suppresses the dwarf phenotype of qua2-1, suggesting that AtPRX71 contributes to the growth defects observed in plants undergoing cell wall damage. Furthermore, AtPRX71 seems to promote the production of reactive oxygen species in qua2-1 plants as well as plants treated with isoxaben. We propose that AtPRX71 contributes to strengthen cell walls, therefore restricting cell expansion, during normal growth and in response to cell wall damage.

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Section: Discussionsupporting

confidence: 64%

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

et al. 2015

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“…Peroxidases are involved in the different physiological functions in the plants, such as peroxide scavenging, lignification, cell wall synthesis, and auxin metabolism [73, 74]. Transgenic experiments showed that both cell elongation and hypocotyl length were suppressed in AtPRX53 (a PEROXIDASE 2 homologous gene)-overexpressing transgenic plants, whereas hypocotyl length was increased in an AtPRX53 mutant [75]. Further analysis confirmed that the effect of AtPRX53 overexpression on cell elongation was associated with a cross-linking reaction in the cell walls.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling to Understand the Effect of Citrus Rootstocks on the Growth of ‘Shatangju’ Mandarin

Liu

et al. 2017

PLoS ONE

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To obtain insight into potential mechanisms underlying the influence of rootstock on scion growth, we performed a comparative analysis of ‘Shatangju’ mandarin grafted onto 5 rootstocks: Fragrant orange (Citrus junons Sieb. ex. Tanaka), Red tangerine (Citrus reticulata Blanco), ‘Shatangju’ mandarin (Citrus reticulata Blanco), Rough lemon (Citrus jambhiri Lush) and Canton lemon (Citrus limonia Osbeck). The tree size of ‘Shatangju’ mandarin grafted onto Canton lemon and Rough lemon were the largest, followed by self-rooted rootstock trees, and the lowest tree sizes correspond to ones grafted on Red tangerine and Fragrant orange rootstocks. The levels of indoleacetic acid (IAA) and gibberellin (GA) were significantly and positively related to growth vigor. The differences of gene expression in leaves of trees grafted onto Red tangerine, Canton lemon and ‘Shatangju’ mandarin were analyzed by RNA-Seq. Results showed that more differentially expressed genes involved in oxidoreductase function, hormonal signal transduction and the glycolytic pathway were enriched in ‘Red tangerine vs Canton lemon’. qRT-PCR analysis showed that expression levels of ARF1, ARF8, GH3 and IAA4 were negatively correlated with the growth vigor and IAA content. The metabolism of GA was influenced by the differential expression of KO1 and GA2OX1 in grafted trees. In addition, most of antioxidant enzyme genes were up-regulated in leaves of trees grafted onto Red tangerine, resulting in a higher peroxidase activity. We concluded that different rootstocks significantly affected the expression of genes involved in auxin signal transduction pathway and GA biosynthesis pathway in the grafted plants, and then regulated the hormone levels and their signal pathways.

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“…AtPrx64 (Lee et al, 2013), AtPrx02/25/ 71 (Shigeto et al, 2013), AtPrx37 (Pedreira et al, 2011) and AtPrx72 (Herrero et al, 2013) have been attributed a role in cell wall stiffening, mostly through lignin polymerization in cell walls of various cell types. On the other hand, AtPrx36 (Kunieda et al, 2013), AtPrx33/34 (Passardi et al, 2006) and AtPrx53 (Jin et al, 2011) have been suggested to play roles in wall loosening based on the phenotype of the corresponding mutants. Their CW targets have not been identified, but CW loosening could be due to the breaking of polysaccharides bonds.…”

Section: Reverse Genetic Studies: Recent Discovery Of CIII Prx Functionsmentioning

confidence: 98%

Roles of cell wall peroxidases in plant development

et al. 2015

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Arabidopsis peroxidase AtPRX53 influences cell elongation and susceptibility toHeterodera schachtii

Cited by 35 publications

References 71 publications

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

The Arabidopsis thaliana Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.

Transcriptome Profiling to Understand the Effect of Citrus Rootstocks on the Growth of ‘Shatangju’ Mandarin

Roles of cell wall peroxidases in plant development

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