Arabidopsis Phyllotaxis Is Controlled by the Methyl-Esterification Status of Cell-Wall Pectins

Peaucelle, Alexis; Louvet, Romain; Johansen, Jorunn Nergaard; Höfte, Monica; Laufs, Patrick; Pelloux, Jérôme; Mouille, Grégory

doi:10.1016/j.cub.2008.10.065

Cited by 308 publications

(320 citation statements)

References 31 publications

Supporting

Mentioning

300

Contrasting

Unclassified

Order By: Relevance

“…The regulation of the DM of HG has been implicated in many aspects of plant development (Wolf et al, 2009a), including cell adhesion (Tieman and Handa, 1994;Wen et al, 1999;Mollet et al, 2000;Bouton et al, 2002;Mouille et al, 2007;Durand et al, 2009), adventitious rooting (Guénin et al, 2011), primordia emergence at the shoot apical meristem (Peaucelle et al, 2008(Peaucelle et al, , 2011, fruit ripening (Brummell and Harpster, 2001;Phan et al, 2007), and plant defense (Bethke et al, 2014). Although data have shown an important role for Figure 5.…”

Section: Discussionmentioning

confidence: 99%

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

et al. 2014

View full text Add to dashboard Cite

Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling, and biochemical analyses was used on wild-type and Atpme48 mutant plants. We showed that AtPME48 is specifically expressed in the male gametophyte and is the second most expressed PME in dry and imbibed pollen grains. Pollen grains from homozygous mutant lines displayed a significant delay in imbibition and germination in vitro and in vivo. Moreover, numerous pollen grains showed two tips emerging instead of one in the wild type. Immunolabeling and Fourier transform infrared analyses showed that the degree of methylesterification of the homogalacturonan was higher in pme482/2 pollen grains. In contrast, the PME activity was lower in pme482/2, partly due to a reduction of PME48 activity revealed by zymogram. Interestingly, the wild-type phenotype was restored in pme482/2 with the optimum germination medium supplemented with 2.5 mM calcium chloride, suggesting that in the wild-type pollen, the weakly methylesterified homogalacturonan is a source of Ca 2+ necessary for pollen germination. Although pollen-specific PMEs are traditionally associated with pollen tube elongation, this study provides strong evidence that PME48 impacts the mechanical properties of the intine wall during maturation of the pollen grain, which, in turn, influences pollen grain germination.

show abstract

Section: Discussionmentioning

confidence: 99%

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Because the degree of HG methylation affects the stiffness of the cell wall (Peaucelle et al, 2008), the LM20 and JIM7 antibodies were used to look at the highly methylesterified HG distribution ( Figure 5; Supplemental Figure 8). An increase in LM20 labeling in AM was observed in the uuat1-2 mutant when compared with wild-type Col-0 (Figures 5A and 5B), suggesting the (A) Immunoblot of the yeast microsomal fractions used to make the proteoliposomes.…”

Section: Absence Of Uuat1 Has Pleiotropic Effects On Seed Coat Cell Wmentioning

confidence: 99%

UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage

et al. 2017

View full text Add to dashboard Cite

“…Four PMEIs have been functionally characterized in Arabidopsis to date. PMEI1 and PMEI2 (Wolf et al, 2003) are highly expressed in flowers; PMEI1 expression is specific to pollen, while the expression of PMEI3 is particularly high in the apical meristem (Peaucelle et al, 2008). PMEI4 is up-regulated during growth acceleration in skotomorphic hypocotyls (Pelletier et al, 2010).…”

Section: Oe Of a Novel Pmei As A System To Study Pme Functionmentioning

confidence: 99%

Demethylesterification of Cell Wall Pectins in Arabidopsis Plays a Role in Seed Germination

et al. 2012

View full text Add to dashboard Cite

The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed germination requires cell wall extensibility changes in both the radicle itself and in the micropylar tissues surrounding the radicle. In wild-type seeds of Arabidopsis (Arabidopsis thaliana), PME activities peaked around the time of testa rupture but declined just before the completion of germination (endosperm weakening and rupture). We overexpressed an Arabidopsis PME inhibitor to investigate PME involvement in seed germination. Seeds of the resultant lines showed a denser methylesterification status of their cell wall homogalacturonans, but there were no changes in the neutral sugar and uronic acid composition of the cell walls. As compared with wild-type seeds, the PME activities of the overexpressing lines were greatly reduced throughout germination, and the low steady-state levels neither increased nor decreased. The most striking phenotype was a significantly faster rate of germination, which was not connected to altered testa rupture morphology but to alterations of the micropylar endosperm cells, evident by environmental scanning electron microscopy. The transgenic seeds also exhibited an apparent reduced sensitivity to abscisic acid with respect to its inhibitory effects on germination. We speculate that PME activity contributes to the temporal regulation of radicle emergence in endospermic seeds by altering the mechanical properties of the cell walls and thereby the balance between the two opposing forces of radicle elongation and mechanical resistance of the endosperm.

show abstract

Arabidopsis Phyllotaxis Is Controlled by the Methyl-Esterification Status of Cell-Wall Pectins

Cited by 308 publications

References 31 publications

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

PECTIN METHYLESTERASE48 Is Involved in Arabidopsis Pollen Grain Germination

UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage

Demethylesterification of Cell Wall Pectins in Arabidopsis Plays a Role in Seed Germination

Contact Info

Product

Resources

About