Arabidopsis response to low-phosphate conditions includes active changes in actin filaments and PIN2 polarization and is dependent on strigolactone signalling

Kumar, Manoj; Pandya-Kumar, Nirali; Dam, Anandamoy; Haor, Hila; Mayzlish-Gati, Einav; Belausov, Eduard; Wininger, Smadar; Abu-Abied, Mohamad; McErlean, Christopher S. P.; Bromhead, Liam J.; Prandi, Cristina; Kapulnik, Yoram; Koltai, Hinanit

doi:10.1093/jxb/eru513

Cited by 44 publications

(30 citation statements)

References 61 publications

(100 reference statements)

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“…PIN2 degradation in vacuoles is suppressed under phosphorus deficiency or treatment with phospholipase Dderived phosphatidic acid Membrane-localized PIN2 is degraded in the vacuole. Given that, under LP conditions, root hair elongation is reduced and auxin transport from the root tip to the shoot is blocked in pin2 seedlings but PIN2 expression is not significantly altered (Kumar et al, 2015;Bhosale et al, 2018), we examined the PIN2 degradation under both the NP and LP conditions. Indeed, observation of the fluorescence of PIN2-GFP fusion protein revealed the increased accumulation of PIN2 in the vacuole under NP conditions ( Fig.…”

Section: Pin2 Deficiency Results In Defective Root Hair Elongation Unmentioning

confidence: 99%

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Lin

Yao

et al. 2019

New Phytologist

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Summary Root hair development is crucial for phosphate absorption, but how phosphorus deficiency affects root hair initiation and elongation remains unclear. We demonstrated the roles of auxin efflux carrier PIN‐FORMED2 (PIN2) and phospholipase D (PLD)‐derived phosphatidic acid (PA), a key signaling molecule, in promoting root hair development in Arabidopsis thaliana under a low phosphate (LP) condition. Root hair elongation under LP conditions was greatly suppressed in pin2 mutant or under treatment with a PLDζ2‐specific inhibitor, revealing that PIN2 and polar auxin transport and PLDζ2‐PA are crucial in LP responses. PIN2 was accumulated and degraded in the vacuole under a normal phosphate (NP) condition, whereas its vacuolar accumulation was suppressed under the LP or NP plus PA conditions. Vacuolar accumulation of PIN2 was increased in pldζ2 mutants under LP conditions. Increased or decreased PIN2 vacuolar accumulation is not observed in sorting nexin1 (snx1) mutant, indicating that vacuolar accumulation of PIN2 is mediated by SNX1 and the relevant trafficking process. PA binds to SNX1 and promotes its accumulation at the plasma membrane, especially under LP conditions, and hence promotes root hair development by suppressing the vacuolar degradation of PIN2. We uncovered a link between PLD‐derived PA and SNX1‐dependent vacuolar degradation of PIN2 in regulating root hair development under phosphorus deficiency.

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Section: Pin2 Deficiency Results In Defective Root Hair Elongation Unmentioning

confidence: 99%

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Lin

Yao

et al. 2019

New Phytologist

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“…Being, PINs polar localization in PM is primarily determined by the constitutive trafficking of PIN vesicles between the PM and endosomes which requires F-actin bundling. 40 Kumar et al 13 studied this and observed a significant reduction in the accumulation of BFA bodies, reduced movement of ARA7labeled endosomes and increased actin bundling under low Pi condition in WT but not in max2-1. In addition, mutants for MAX2, MAX4, PIN2 and TIR3 (required for polar auxin transport) and one ACTIN2 mutant line had a reduced response (in term of root hair density) to low Pi compared with WT.…”

Section: Sl-associated Root Development Involve Changes In Auxin Efflmentioning

confidence: 96%

“…Recently, Kumar et al 13 reported that SL signaling under low Pi condition (at least during early developmental stage, 48 hpg) transmits in a MAX2 dependent manner, 48 also involve regulation of PIN2 polar localization in PM and actin bundling and dynamics in Arabidopsis. In Kumar et al 13 studies, Arabidopsis seedlings under low Pi condition showed reduced PIN2 trafficking and polarization in the PM (similar to the results presented for PIN2 and 7 in Gonzalez-Mendoza et al 50 ), decreased ARA7labeled endosome trafficking, and increased actin filament bundling in root cells of WT. The max4-1, but not max2-1, with supplementation of synthetic SL (GR24) exhibited depletion of PIN2 from the PM under low-Pi conditions.…”

Section: Sl-associated Root Development Involve Changes In Auxin Efflmentioning

confidence: 99%

“…5 It is only recently demonstrated that SLs are involved in orchestrating the shoot architecture by acting as shoot branching suppressor [6][7][8] and root architecture, by regulating lateral and adventitious root formation, and root hair development. [9][10][11][12][13] SLs are synthesized mainly in root and other plant parts such as epicotyl and internode tissue; however the identified SLs to date are most abundant in roots. 3 Natural SLs share a common tricyclic lactone structure consisting of 3 rings (ABC), connected to a D-ring butenolide group via an enol-ether bridge.…”

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confidence: 99%

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Strigolactone signaling in root development and phosphate starvation

Kumar

Pandya-Kumar

Kapulnik

et al. 2015

Plant Signaling & Behavior

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Strigolactones (SLs), have recently been recognized as phytohormone involve in orchestrating shoot and root architecture. In, roots SLs positively regulate root hair length and density, suppress lateral root formation and promote primary root meristem cell number. The biosynthesis and exudation of SLs increases under low phosphate level to regulate root responses. This hormonal response suggests an adaptation strategy of plant to optimize growth and development under nutrient limitations. However, little is known on signal-transduction pathways associated with SL activities. In this review, we outline the current knowledge on SL biology by describing their role in the regulation of root development. Also, we discuss the recent findings on the non-cell autonomous signaling of SLs, that involve PIN polarization, vesicle trafficking, changes in actin architecture and dynamic in response to phosphate starvation.

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“…Transcriptome analysis revealed that SLs inhibit bud growth by negatively regulating auxin transport in rice [156]. SLs regulate root development by inhibiting the transport of auxin from shoots to roots and auxin flux within root tissues, and regulate branching by affecting polar auxin transport (PAT)/canalization in rice [157][158][159]. When auxin is depleted, SL can inhibit bud outgrowth in pea.…”

Section: Sl or Kar Crosstalk With Auxinmentioning

confidence: 99%

Comparing and Contrasting the Multiple Roles of Butenolide Plant Growth Regulators: Strigolactones and Karrikins in Plant Development and Adaptation to Abiotic Stresses

Tao

Lian

Wang

2019

IJMS

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Strigolactones (SLs) and karrikins (KARs) are both butenolide molecules that play essential roles in plant growth and development. SLs are phytohormones, with SLs having known functions within the plant they are produced in, while KARs are found in smoke emitted from burning plant matter and affect seeds and seedlings in areas of wildfire. It has been suggested that SL and KAR signaling may share similar mechanisms. The α/β hydrolases DWARF14 (D14) and KARRIKIN INSENSITIVE 2 (KAI2), which act as receptors of SL and KAR, respectively, both interact with the F-box protein MORE AXILLARY GROWTH 2 (MAX2) in order to target SUPPRESSOR OF MAX2 1 (SMAX1)-LIKE/D53 family members for degradation via the 26S proteasome. Recent reports suggest that SLs and/or KARs are also involved in regulating plant responses and adaptation to various abiotic stresses, particularly nutrient deficiency, drought, salinity, and chilling. There is also crosstalk with other hormone signaling pathways, including auxin, gibberellic acid (GA), abscisic acid (ABA), cytokinin (CK), and ethylene (ET), under normal and abiotic stress conditions. This review briefly covers the biosynthetic and signaling pathways of SLs and KARs, compares their functions in plant growth and development, and reviews the effects of any crosstalk between SLs or KARs and other plant hormones at various stages of plant development. We also focus on the distinct responses, adaptations, and regulatory mechanisms related to SLs and/or KARs in response to various abiotic stresses. The review closes with discussion on ways to gain additional insights into the SL and KAR pathways and the crosstalk between these related phytohormones.factors through interactions between the phytohormone regulatory networks via the perception and signal transduction originating at various receptors [20][21][22][23].Strigolactones (SLs) were originally isolated from root exudates of cotton and as seed germination stimulants from plants in the Orobanchaceae family that parasitize plant roots (Striga, Phelipanche, and Orobanche spp.) [24][25][26]. SLs normally control seed germination and seedling development [27], shoot branching [28-32], root architecture [33], and leaf senescence [34]. SLs also promote beneficial symbiotic relationships between host plants and mycorrhizal fungi [35,36]. The biosynthesis and signaling of SLs are regulated by various abiotic stress factors [37][38][39][40], including the recently reported SL involvement in responding to nutrient deprivation, drought, chilling and salinity [38,[40][41][42][43][44][45][46][47][48][49][50]. Such studies provide new insights into the novel roles SL signaling plays in the regulation of plant adaptation to adverse environmental conditions [51][52][53][54].Karrikins (KARs) are found in smoke released from the heating or combustion of plant material, after which they can stimulate the germination of dormant seeds [55][56][57][58]. KARs are also involved in the inhibition of hypocotyl elongation and in the promotion of cotyledon expansion and...

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Arabidopsis response to low-phosphate conditions includes active changes in actin filaments and PIN2 polarization and is dependent on strigolactone signalling

Abstract: HighlightThis study provides an insight into the cellular events of cytoskeleton rearrangement, vesicle trafficking, and PIN protein localization that are associated with low-Pi response and are dependent on strigolactone-auxin cross-talk.

Cited by 44 publications

References 61 publications

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Strigolactone signaling in root development and phosphate starvation

Comparing and Contrasting the Multiple Roles of Butenolide Plant Growth Regulators: Strigolactones and Karrikins in Plant Development and Adaptation to Abiotic Stresses

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