Arabidopsis Serine Decarboxylase 1 (SDC1) in Phospholipid and Amino Acid Metabolism

Liu, Yu-chi; Gunawan, Farrel; Yunus, Ian Sofian; Nakamura, Yuki

doi:10.3389/fpls.2018.00972

Cited by 13 publications

(10 citation statements)

References 19 publications

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“…9). The initial reaction step is the conversion of Ser to Etn by SERINE DECARBOXYLASE 1 (SDC1; Yunus et al, 2016;Liu et al, 2018a). The product Etn is then phosphorylated to PEtn by CHOLINE/ ETHANOLAMINE KINASE 4 (CEK4; Lin et al, 2015).…”

Section: An Updated Metabolic Map Of Pc Biosynthesismentioning

confidence: 99%

A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth

et al. 2018

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Phosphatidylcholine (PC) is a primary class of membrane lipids in most eukaryotes. In plants, the primary PC biosynthetic pathway and its role in plant growth and development remain elusive due to lack of a mutant model with substantially decreased PC content. Recently, a double mutant of Arabidopsis (Arabidopsis thaliana) PHOSPHO-BASE N-METHYLTRANSFERASE 1 (PMT1) and PMT3 was reported with reduced PC content and defective plant growth. However, residual PC content as well as the nonlethal phenotype of the mutant suggests an additional enzyme contributes to PC biosynthesis. In this article, we report on the role of three PMTs in PC biosynthesis and plant development, with a focus on PMT2. PMT2 had the highest expression level among the three PMTs, and it was highly expressed in roots. The pmt1 pmt2 double mutant enhanced the defects in root growth, cell viability, and PC content of pmt1, suggesting that PMT2 functions together with PMT1 in roots. Chemical inhibition of PMT activity in wild-type roots reproduced the short root phenotype observed in pmt1 pmt2, suggesting that PMT1 and PMT2 are the major PMT isoforms in roots. In shoots, pmt1 pmt2 pmt3 enhanced the phenotype of pmt1 pmt3, showing seedling lethality and further reduced PC content without detectable de novo PC biosynthesis. These results suggest that PMTs catalyze an essential reaction step in PC biosynthesis and that the three PMTs have differential tissue-specific functions in PC biosynthesis and plant growth.

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Section: An Updated Metabolic Map Of Pc Biosynthesismentioning

confidence: 99%

A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth

et al. 2018

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“…However, several other proteins were also highlighted, such as the serine decarboxylase (SDC). SDC is responsible for conversion of serine to ethanolamine, a phospholipid precursor in plants 93 . Phospholipids are essential components of biological membranes and signal transduction cascades in plants 94 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa

Crizel¹,

Perin

Vighi

et al. 2020

Sci Rep

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Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragaria x ananassa CDPK gene family was performed, including gene structures, phylogeny, interactome and expression profiles. Nine new CDPK genes in Fragaria x ananassa were identified based on RNA-seq data. These identified strawberry FaCDPK genes were classified into four main groups, based on the phylogenetic analysis and structural features. FaCDPK genes were differentially expressed during fruit development and ripening, as well as in response to abiotic stress (salt and drought), and hormone (abscisic acid) treatment. In addition, the interaction network analysis pointed out proteins involved in the ABA-dependent response to plant stress via Ca 2+ signaling, especially RBOHs. To our knowledge, this is the first report on CDPK families in Fragaria x ananassa, and it will provide valuable information for development of biofortified fruits and stress tolerant plants. Plants are constantly exposed to stress conditions, such as drought, low or high temperature, and high salinity 1. Plants adapt to these conditions by capturing external signals and modulating their responses using complex mechanisms. These mechanisms involve the perception of the stimulus and subsequent signal transduction, which lead to the activation of various chemical, molecular, biochemical, and physiologic changes, improving plant plasticity 2. The calcium ion (Ca 2+) plays central roles in the regulation of different physiological processes in plants as an important secondary messenger. Transient changes in the cytoplasmic concentration of Ca 2+ are detected by several types of sensor proteins that initiate rapid signal transduction processes by triggering cascades of phosphorylation events. Several major classes of Ca 2+ binding proteins have been characterized in plants, including Ca 2+-dependent protein kinases (CDPK), calmodulin (CaM), CAM-like proteins (CML), and calcineurin B-like proteins (CBL) 3-6. Among these, CDPK constitutes a large calcium-sensing family only found in plants, protists, oomycetes, and green algae, and not in animals and fungi 7. CDPK structures include four domains: N-terminal, self-regulatory/autoinhibitory, serine/threonine kinase, and finally calmodulin-like regulatory domains (CaM-LD) 8. The CaM-LD domain contains three or four EFhand Ca 2+-binding motifs that recognize distinct Ca 2+ signatures with variable affinities. The C-terminal lobe of the CaM-LD binds Ca 2+ with high affinity. At low Ca 2+ levels, the structure is stabilized by the interaction of this lobe with the auto-inhibitory region of the protein. Otherwise, a conformational change induced by the binding of Ca 2+ to the low-affinity N-terminal lobe of CaM-LD results in the release of the auto-inhibition 9,10. CDPKs are involved in stress signaling, hormone respon...

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“…The ethanolamine, which is the first step in PC biosynthesis [58] . Choline /ethanolamine kinase (CEK: A0A0K9RLT3) catalyzes the initial reaction step of choline metabolism that produces phosphoethanolamine [59] .…”

Section: Analysis Of Daps Related To Metabolismmentioning

confidence: 99%

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

Cui

Cheng

et al. 2020

Preprint

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Background Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of beet salt-tolerant. Results Here, leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data. Conclusions Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.

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Arabidopsis Serine Decarboxylase 1 (SDC1) in Phospholipid and Amino Acid Metabolism

Cited by 13 publications

References 19 publications

A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth

A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth

Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

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