Arabidopsis thaliana intramembrane proteases

Abstract

Proteolysis is considered as a crucial factor determining the proper development of the plant and its efficient functioning in variable environmental conditions. The role of proteases in protein quality control and protein turnover processes is well documented. The results of studies performed in recent years reveal; however, that proteolytic enzymes also participate in signal transduction pathways by releasing membrane-anchored transcription factors in the process known as regulated intramembrane proteolysis (RIP). The first described intramembrane protease was identified in human cells in 1997. In turn, the first plant intramembrane protease was identified in 2005, in Arabidopsis thaliana. To date, most studies concerning the RIP process in plants have been performed on this model plant. The knowledge concerning the potential physiological role of RIP is very limited. However, continuously accumulating information concerning this issue indicates that RIP, like the other proteolytic mechanisms, has a significant effect on plant ontogenesis, acclimatization and fertility. The aim of this article is to gather and systemize the present knowledge concerning the intramembrane proteases in A. thaliana.

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies

“…These new subunits have particularly high CF-MS scores for interaction with NDH subcomplex B and L members and, in some cases, score higher than interactions among known members of the NDH complex (Figure 6D, network). EGY1 and EGY2 are chloroplast-localized intramembrane metalloproteases, but their specific functions remain unknown (Adamiec et al, 2017). The specific enrichment of EGY1, EGY2, and the NDH complex in bundle sheath chloroplasts of C4 plants supports the association of these metalloproteases with NDH in vivo (Majeran et al, 2008).…”

Identification of differentially expressed transcripts for trunk formation in sago palm using annealing control primer GeneFishing technique

“…The DETs encoding for these proteins are from the NT sample, with higher band intensity of 123% and 47%, respectively. The membrane-associated zinc metallopeptidase is suggested to have cleaving functions at the trans-membrane [59]. Several homologs of the metallopeptidase have been identified in Arabidopsis using the bioinformatics approach [60].…”

A pan-plant protein complex map reveals deep conservation and novel assemblies

“…These new subunits have high CF-MS scores for interactions with multiple subunits of the NDH complex, scoring highly with NDH subcomplex B and L members, and in some cases scoring higher than the interactions between known members of the NDH complex itself ( Figure 6D, network ). EGY1 and EGY2 are chloroplast-localized intramembrane metalloproteases, but their speci ic functions remain unknown (Adamiec et al, 2017) . The speci ic enrichment of EGY1, EGY2, and the NDH complex in Bundle Sheath chloroplasts of C4 plants supports the association of these metalloproteases with NDH in vivo (Majeran et al, 2008) .…”