Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Zentgraf, Ulrike; Doll, Jasmin

doi:10.3390/plants8120578

Cited by 53 publications

(45 citation statements)

References 105 publications

(121 reference statements)

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“…Many individual TFs have also been shown to regulate LS; for example, NAP, WRKY53, WRKY75, and ORE1 are positive regulators and JUB1, WRKY54, and WRKY70 negatively regulate LS (Guo et al, 2017;Lei et al, 2020;Miao et al, 2004;Qiu et al, 2015;Zentgraf & Doll, 2019).…”

Section: Introductionmentioning

confidence: 99%

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Brusslan

2020

Plant Direct

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In plants, the vegetative to reproductive phase transition (termed bolting in Arabidopsis) generally precedes age‐dependent leaf senescence (LS). Many studies describe a temporal link between bolting time and LS, as plants that bolt early, senesce early, and plants that bolt late, senesce late. The molecular mechanisms underlying this relationship are unknown and are potentially agriculturally important, as they may allow for the development of crops that can overcome early LS caused by stress‐related early‐phase transition. We hypothesized that leaf gene expression changes occurring in synchrony with bolting were regulating LS. ARABIDOPSIS TRITHORAX (ATX) enzymes are general methyltransferases that regulate the adult vegetative to reproductive phase transition. We generated an atx1, atx3, and atx4 (atx1,3,4) triple T‐DNA insertion mutant that displays both early bolting and early LS. This mutant was used in an RNA‐seq time‐series experiment to identify gene expression changes in rosette leaves that are likely associated with bolting. By comparing the early bolting mutant to vegetative WT plants of the same age, we were able to generate a list of differentially expressed genes (DEGs) that change expression with bolting as the plants age. We trimmed the list by intersection with publicly available WT datasets, which removed genes from our DEG list that were atx1,3,4 specific. The resulting 398 bolting‐associated genes (BAGs) are differentially expressed in a mature rosette leaf at bolting. The BAG list contains many well‐characterized LS regulators (ORE1, WRKY45, NAP, WRKY28), and GO analysis revealed enrichment for LS and LS‐related processes. These bolting‐associated LS regulators may contribute to the temporal coupling of bolting time to LS.

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Section: Introductionmentioning

confidence: 99%

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Brusslan

2020

Plant Direct

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“…Gene regulatory networks connect various WRKY transcription factors ( Figure 6 D). WRKY53 is a main component of a network that controls early senescence that is regulated by hydrogen peroxide [ 71 ]. It is regulated by various mechanisms and connects senescence with stress responses.…”

Section: Resultsmentioning

confidence: 99%

“… Schematic representation of gene networks regulating leaf dynamics showing differentially expressed genes in cid3cid4 . Networks of NAC transcription factors and WRKYs based on previously reported work ( A ) [ 66 , 67 ], ( B ) [ 67 ], ( C ) [ 68 , 69 , 70 ], ( D ) [ 63 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ]. NAC scale includes ( A ), ( B ), and ( C ).…”

Section: Figurementioning

confidence: 99%

The ATXN2 Orthologs CID3 and CID4, Act Redundantly to In-Fluence Developmental Pathways throughout the Life Cycle of Arabidopsis thaliana

López-Juárez

Aguilar-Henonin

Guzmán

2021

IJMS

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RNA-binding proteins (RBPs) are key elements involved in post-transcriptional regulation. Ataxin-2 (ATXN2) is an evolutionarily conserved RBP protein, whose function has been studied in several model organisms, from Saccharomyces cerevisiae to the Homo sapiens. ATXN2 interacts with poly(A) binding proteins (PABP) and binds to specific sequences at the 3′UTR of target mRNAs to stabilize them. CTC-Interacting Domain3 (CID3) and CID4 are two ATXN2 orthologs present in plant genomes whose function is unknown. In the present study, phenotypical and transcriptome profiling were used to examine the role of CID3 and CID4 in Arabidopsis thaliana. We found that they act redundantly to influence pathways throughout the life cycle. cid3cid4 double mutant showed a delay in flowering time and a reduced rosette size. Transcriptome profiling revealed that key factors that promote floral transition and floral meristem identity were downregulated in cid3cid4 whereas the flowering repressor FLOWERING LOCUS C (FLC) was upregulated. Expression of key factors in the photoperiodic regulation of flowering and circadian clock pathways, were also altered in cid3cid4, as well as the expression of several transcription factors and miRNAs encoding genes involved in leaf growth dynamics. These findings reveal that ATXN2 orthologs may have a role in developmental pathways throughout the life cycle of plants.

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“…On the other hand, WRKY TFs regulate the expression of downstream genes involved in general features of senescence, as remobilization processes and induction of antioxidant enzymes. In fact, direct interaction of WRKY53 with the promoter of catalase genes has been reported [ 73 ]. Accordingly, we found induction of catalase activity only in drought-stressed leaves from PMB-0220, agreeing with the induction of PvWRKY53 expression in these plants, but not in the PHA-0683 ones.…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of Drought Responses in Two Phaseolus vulgaris Genotypes

López

Pineda

Alamillo

2020

Plants

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Drought is probably the most harmful stress affecting common bean crops. Domestication, worldwide spread and local farming practices has entailed the development of a wide variety of common bean genotypes with different degrees of resistance to water stress. In this work, physiological and molecular responses to water stress have been compared in two common bean accessions, PHA-0683 and PMB-0220, previously identified as highly and moderately resistant to water stress, respectively. Our hypothesis was that only quantitative differences in the expression patterns of key genes should be found if molecular mechanisms regulating drought resistance are similar in the two accessions. However, results presented here indicate that the resistance to drought in PMB-0220 and PHA-0683 common bean accessions is regulated by different molecular mechanisms. Differential regulation of ABA synthesis and ABA signaling related genes among the two genotypes, and the control of the drought-induced senescence have a relevant contribution to the higher resistance level of PHA-0683 accession. Our results also suggest that expression patterns of key senescence-related transcription factors could be considered in the screening for drought resistance in common bean germplasm collections.

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Arabidopsis WRKY53, a Node of Multi-Layer Regulation in the Network of Senescence

Cited by 53 publications

References 105 publications

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

The ATXN2 Orthologs CID3 and CID4, Act Redundantly to In-Fluence Developmental Pathways throughout the Life Cycle of Arabidopsis thaliana

Differential Regulation of Drought Responses in Two Phaseolus vulgaris Genotypes

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