Arabidopsis YL1/BPG2 Is Involved in Seedling Shoot Response to Salt Stress through ABI4

Li, Pengcheng; Huang, Jinguang; Yu, Shao-Wei; Sun, Peng; Wu, Changai; Zheng, Chengchao

doi:10.1038/srep30163

Cited by 17 publications

(24 citation statements)

References 63 publications

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“…RNA extraction and cDNA synthesis experiments were performed as described previously24. The qRT-PCR experiments were performed using a CFX96 real-time PCR system (Bio-Rad, C1000) with SYBR green real-time PCR master mix (Takara).…”

Section: Methodsmentioning

confidence: 99%

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

et al. 2016

Sci Rep

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MDN1/Rea1, as an AAA-type ATPase, is predicted to be the largest protein involved in pre-ribosome maturation in most organisms. However, its function in plant growth and development is poorly understood. Here, we characterized a novel Arabidopsis mutant, dwarf & short root (dsr) 1, which shows pleiotropic developmental phenotypes, such as slow germination, short root, dwarf shoot, and reduced seed set under normal growth conditions. Using positional cloning, we revealed that the AtMDN1 function is impaired by a ‘glutamic acid’ to ‘lysine’ change at position 3838 of the amino acid sequence in dsr1. Multiple sequence alignment analysis revealed that the mutated Glu residue, which located in the linker domain of AtMDN1, is extremely conserved among organisms. AtMDN1 is expressed in various tissues, particularly in the shoot apex and root tip. Moreover, the results of transcript profile analyses showed that the dysfunction of AtMDN1 in dsr1 impairs the expression of genes related to plant growth and development, which is tightly associated with the pleiotropic phenotypes of dsr1. Thus, we concluded that the Glu residue plays a vital role in maintaining AtMDN1 functions, which are essential for plant growth and development.

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Section: Methodsmentioning

confidence: 99%

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

et al. 2016

Sci Rep

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“…ABI4-regulated individual genes and transcriptomes have been reported in a variety of contexts, including seed development, dormancy and germination (Nakabayashi et al, 2005;Penfield et al, 2006;Shu et al, 2013), oxidative stress response (Kerchev et al, 2011), ABAtreatment of ectopic expression lines (Reeves et al, 2011), salt stress response (Li et al, 2016), and retrograde signaling from organelles (Koussevitzky et al, 2007;Giraud et al, 2009;Leon et al, 2012). In dry seeds, expression of over 2200 genes was reduced at least two-fold in abi4 mutants, while over 2800 genes were expressed at least two-fold higher in this background (Nakabayashi et al, 2005), reflecting primarily repressive action of ABI4.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of Serine114 of the transcription factor ABSCISIC ACID INSENSITIVE 4 is essential for activity

Eisner

Maymon

Sánchez

et al. 2020

Preprint

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The transcription factor ABA-INSENSITIVE(ABI)4 has diverse roles in regulating plant growth, including inhibiting germination and reserve mobilization in response to ABA and high salinity, inhibiting seedling growth in response to high sugars, inhibiting lateral root growth, and repressing light-induced gene expression. ABI4 activity is regulated at multiple levels, including gene expression, protein stability, and activation by phosphorylation. Although ABI4 can be phosphorylated at multiple residues by MAPKs, we found that S114 is the preferred site of MPK3. To examine the possible biological role of S114 phosphorylation, we transformed abi4-1 mutant plants with ABI4pro::ABI4 constructs encoding wild type (114S), phosphorylation-null (S114A) or phosphomimetic (S114E) forms of ABI4. Phosphorylation of S114 is both necessary and sufficient for the response to ABA, glucose, salt stress, and lateral root development, where the abi4 phenotype could be complemented by expressing ABI4(114S) or ABI4(S114E) but not ABI4(S114A). Comparison of root transcriptomes in ABA-treated roots of abi4-1 mutant plants transformed with constructs encoding the different phosphorylation-forms of S114 of ABI4 revealed that 85% of the ABI4-regulated genes whose expression pattern could be restored by expressing ABI4(114S) are down-regulated by ABI4. Over half of the ABI4-modulated genes were independent of the phosphorylation state of ABI4; these are enriched for stress responses. Phosphorylation of S114 was required for regulation of 35% of repressed genes, but only 17% of induced genes. The genes whose repression requires the phosphorylation of S114 are mainly involved in embryo and seedling development, growth and differentiation, and regulation of gene expression.

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“…73 genes could nd at least one homolog in A. thaliana genome, whereas 19 genes did not hit any A. thaliana genes. Four genes, SIN_1008253, SIN_1008230, SIN_1008220 and SIN_1008205, encoding GTP binding domain, FAD/NAD(P)-binding domain, RNA recognition motif domain and Zinc nger (A20-type) respectively, were homologous to Arabidopsis genes whose functional roles in response to drought stress had been validated through mutagenesis or transgenic studies [24][25][26][27]. Two of them, SIN_1008253 and SIN_1008220 contained nonsynonymous substitutions in the exons between two parents (Additional le 1: Table S3).…”

Section: Putative Candidate Genes Of Major Qtls For Drought Tolerancementioning

confidence: 99%

“…Two of them, SIN_1008253 and SIN_1008220 contained nonsynonymous substitutions in the exons between two parents (Additional le 1: Table S3). SIN_1008253, homologous with AT3G57180, which encodes a chloroplast localized protein YL1/BPG2 that involved in seedling shoot response to salt stress [24]. Loss of YL1 function caused Na + accumulation and hypersensitive phenotype of shoot under salt stress [24].…”

Section: Putative Candidate Genes Of Major Qtls For Drought Tolerancementioning

confidence: 99%

“…SIN_1008253, homologous with AT3G57180, which encodes a chloroplast localized protein YL1/BPG2 that involved in seedling shoot response to salt stress [24]. Loss of YL1 function caused Na + accumulation and hypersensitive phenotype of shoot under salt stress [24]. SIN_1008230 harbored one SNP and a 17bp InDel in the promoter region (at the position of <-1kb from start codon).…”

Section: Putative Candidate Genes Of Major Qtls For Drought Tolerancementioning

confidence: 99%

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Construction of a high density genetic map and QTL mapping of PEG-induced drought tolerance at the early seedling stage in sesame using whole genome re-sequencing

Liang¹,

Ye²,

Yan³

et al. 2020

Preprint

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BackgroundImprovement in sesame (Sesamum indicum L.) drought tolerance at seedling stage is important for yield stability. Genetic approaches combing with conventional breeding is the most effective way to develop drought-tolerant cultivars. So far, only a few studies have been reported to reveal gene/ quantitative trait loci (QTL) controlling drought tolerance in sesame. To identify the genomic regions associated with drought tolerance, we constructed a high-density genetic map using a recombinant inbred line (RIL) population through whole genome re-sequencing (WGRS) technique. QTLs contributing to three seedling traits were identified under both non-stress and water stress conditions.ResultsThree drought tolerance related traits and their relative values (the ratio of value under stress to value under control condition), including seedling weight (SW), shoot length (SL) and root length (RL), were evaluated under control and PEG-induced osmotic conditions at seedling stage in a RIL population derived from cross of Zhushanbai (ZSB) and Jinhuangma (JHM). Significant variation and high broad sense heritability were observed for all traits except SW under stress condition in the population. With this population, a high-density linkage map with 1354 bin markers was constructed through WGRS strategy. Composite interval mapping analysis was performed for all the traits as well as their relative phenotypic data. A total of 34 QTLs were detected for these traits under both conditions and their relative values, and 13 stable QTLs associated with seven traits were revealed in two independent experiments, explaining on average, 4.95-16.26% of phenotypic variation for each QTL. Four of them contributed more than 10% of phenotypic variation. One region on chromosome 12 contained two major QTLs related to RL under osmotic condition and relative RL. Seven candidate genes underlying major QTLs for drought tolerance were identified according to gene descriptions and variations between parents.ConclusionThe current study reports the first QTL mapping of drought tolerance related traits through a RIL population and first QTL detection of root related trait (root length) in sesame. These findings will provide new genetic resources for molecular improvement of drought tolerance and candidate gene identification in sesame.

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Arabidopsis YL1/BPG2 Is Involved in Seedling Shoot Response to Salt Stress through ABI4

Cited by 17 publications

References 63 publications

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

The Mutation of Glu at Amino Acid 3838 of AtMDN1 Provokes Pleiotropic Developmental Phenotypes in Arabidopsis

Phosphorylation of Serine114 of the transcription factor ABSCISIC ACID INSENSITIVE 4 is essential for activity

Construction of a high density genetic map and QTL mapping of PEG-induced drought tolerance at the early seedling stage in sesame using whole genome re-sequencing

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