Arabinogalactan and fructooligosaccharides improve the gut barrier function in distinct areas of the colon in the Simulator of the Human Intestinal Microbial Ecosystem

A Critical Evaluation of In Vitro Hesperidin 2S Bioavailability in a Model Combining Luminal (Microbial) Digestion and Caco‐2 Cell Absorption in Comparison to a Randomized Controlled Human Trial

“…SHIME samples containing the highest quantities of microbial phenolic metabolites (AC and TC of treatment week 1 and 3) were diluted 1:10 in medium to study their transport and phase II metabolism in Caco‐2 cell monolayers. Average TEER values of 315 ± 36 Ω cm 2 did not change during the transport experiment ( p = 0.21), and a visual microscopical investigation of the cells confirmed monolayer integrity . Phenolics were quantified in apical and basolateral compartments before and after 2 h of treatment.…”

“…Average TEER values of 315 ± 36 cm 2 did not change during the transport experiment (p = 0.21), and a visual microscopical investigation of the cells confirmed monolayer integrity. [22] Phenolics were quantified in apical and basolateral compartments before and after 2 h of treatment. Of all compounds identified in the SHIME, only dihydroisoferulic acid transport was significant (Figure 3).…”

Micro- and nano bio-based delivery systems for food applications: In vitro behavior

“…7. This model suffered some modification and actually the five vessels represent the human stomach, small intestine, ascending, transverse and descending colon, incorporating a microbial ecosystem in the large intestine vessels [208,209]. Chaikham et al [208] used this model to assess the survivability of microencapsulated probiotics (i.e., Lactobacillus acidophilus LA5 or Lactobacillus casei 01) suspended in pressurized longan juice.…”

“…Therefore, these in vitro digestion systems can be considered as a preliminary, but still crucial, step towards the study of functional food products digestion [200,204,206,209]. [206]).…”

New Frontiers in Fibers: Innovative and Emerging Research on the Gut Microbiome and Bone Health

“…2). Recent advances in this type of modeling allow researchers to combine the study of bacteria-host interactions-such as mucosal adhesion (M-SHIME) [26] and interaction with the immune system (cocultures of enterocytes and immune cells) [27]-with the continuous model, thereby further increasing the scientific output. The effect of soluble corn fiber (SCF; Promitor SCF 85, Tate & Lyle, Hoffman Estates, IL) has recently been investigated in the SHIME and in vivo and it is used as a case study in this article.…”