Arabinogalactan-Proteins from the Liverwort Marchantia polymorpha L., a Member of a Basal Land Plant Lineage, Are Structurally Different to Those of Angiosperms

Happ, Kathrin; Classen, Birgit

doi:10.3390/plants8110460

Cited by 22 publications

(48 citation statements)

References 64 publications

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“…SEC analysis was performed as described in 68 . For calculation of hydrodynamic volumes, different pullulans with the masses 85.3 × 10 4 Da, 38.0 × 10 4 Da, 18.6 × 10 4 Da, 2.37 × 10 4 Da, 1,22 × 10 4 Da (all from Shodex, Japan), 4.8 × 10 4 Da and 10.0 × 10 4 Da (both Honeywell Fluka, USA) were used.…”

Section: Size-exclusion Chromatography (Sec)mentioning

confidence: 99%

Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Pfeifer

Shafee

Johnson

et al. 2020

Sci Rep

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Seagrasses evolved from monocotyledonous land plants that returned to the marine habitat. This transition was accomplished by substantial changes in cell wall composition, revealing habitat-driven adaption to the new environment. Whether arabinogalactan-proteins (AGPs), important signalling molecules of land plants, are present in seagrass cell walls is of evolutionary and plant development interest. AGPs of Zostera marina L. were isolated and structurally characterised by analytical and bioinformatics methods as well as by ELISA with different anti-AGP antibodies. Calcium-binding capacity of AGPs was studied by isothermal titration calorimetry (ITC) and microscopy. Bioinformatic searches of the Z. marina proteome identified 9 classical AGPs and a large number of chimeric AGPs. The glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues. Although the common backbone structure of land plant AGPs is conserved in Z. marina, the terminating residues are distinct with high amounts of uronic acids. These differences likely result from the glycan-active enzymes (glycosyltransferases and methyltransferases) and are essential for calcium-binding properties. The role of this polyanionic surface is discussed with regard to adaption to the marine environment.

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Section: Size-exclusion Chromatography (Sec)mentioning

confidence: 99%

Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Pfeifer

Shafee

Johnson

et al. 2020

Sci Rep

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“…Although the hydroxyproline content of examined AGP‐like glycoproteins might seem quite low, AGPs with much lower content or even completely lacking hydroxyproline were already described (Bobalek and Johnson 1983, Baldwin et al. 1993, Happ and Classen 2019, Pfeifer et al. 2020).…”

Section: Discussionmentioning

confidence: 98%

“…Unique 3‐ O ‐methyl‐rhamnose was found in AGPs of various mosses, ferns, and liverwort Marchantia polymorpha (Bartels and Classen 2017, Bartels et al. 2017, Happ and Classen 2019). Although no information regarding the presence of methylated hexose in AGPs can be found, exceptional 6‐methyl‐galactofuranose was identified in Chlamydomonas reinhardtii extensins (Bollig et al.…”

Section: Discussionmentioning

confidence: 99%

Arabinogalactan‐like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs

Přerovská

Henke²,

Bleha³

et al. 2021

Journal of Phycology

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Arabinogalactan proteins (AGPs) encompass a diverse group of plant cell wall proteoglycans, which play an essential role in plant development, signaling, plant‐microbe interactions, and many others. Although they are widely distributed throughout the plant kingdom and extensively studied, they remain largely unexplored in the lower plants, especially in seaweeds. Ulva species have high economic potential since various applications were previously described including bioremediation, biofuel production, and as a source of bioactive compounds. This article presents the first experimental confirmation of AGP‐like glycoproteins in Ulva species and provides a simple extraction protocol of Ulva lactuca AGP‐like glycoproteins, their partial characterization and unique comparison to scarcely described Solanum lycopersicum AGPs. The reactivity with primary anti‐AGP antibodies as well as Yariv reagent showed a great variety between Ulva lactuca and Solanum lycopersicum AGP‐like glycoproteins. While the amino acid analysis of the AGP‐like glycoproteins purified by the β‐d‐glucosyl Yariv reagent showed a similarity between algal and land plant AGP‐like glycoproteins, neutral saccharide analysis revealed unique glycosylation of the Ulva lactuca AGP‐like glycoproteins. Surprisingly, arabinose and galactose were not the most prevalent monosaccharides and the most outstanding was the presence of 3‐O‐methyl‐hexose, which has never been described in the AGPs. The exceptional structure of the Ulva lactuca AGP‐like glycoproteins implies a specialized adaptation to the marine environment and might bring new insight into the evolution of the plant cell wall.

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“…AGPs are proteoglycans made of a heavily O-glycosylated (90% of the overall mass) protein backbone (Dehors et al 2019). They are ubiquitous cell wall components in land plants, speculated to be involved in a diversity of vital processes such as differentiation, cell to cell recognition, embryogenesis, programmed cell death, and tip-growth (Happ & Classen 2019). AGPs are hypothesized to be involved in pH-dependent signaling by releasing Ca 2+ as a secondary messenger that regulates development (Lamport & Várnai 2013, Lamport et al 2014.…”

Section: Discussionmentioning

confidence: 99%

Cell wall polymers in the Phaeoceros placenta reflect developmental and functional differences across generations

Henry

Ligrone²,

Vaughn³

et al. 2021

BDE

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The placenta of hornworts is unique among bryophytes in the restriction of transfer cells that are characterized by elaborate wall labyrinths to the gametophyte generation. During development, cells around the periphery of the sporophyte foot elongate, forming smooth-walled haustorial cells that interdigitate with gametophyte cells. Using immunogold labeling with 22 antibodies to diverse cell wall polymers, we examined compositional differences in the developmentally and morphologically distinct cell walls of gametophyte transfer cells and sporophyte haustorial cells in the placenta of Phaeoceros. As detected by Calcofluor White fluorescence, cellulose forms the cell wall scaffolding in cells on both sides of the placenta. Homogalacturonan (HG) and rhamnogalacturonan I (RG-I) pectins are abundant in both cell types, and haustorial cells are further enriched in methyl-esterified HGs. The abundance of pectins in placental cell walls is consistent with the postulated roles of these polymers in cell wall porosity and in maintaining an acidic apoplastic pH favorable to solute transport. Xyloglucan hemicellulose, but not mannans or glucuronoxylans, are present in cell walls at the interface between the two generations with a lower density in gametophytic wall ingrowths. Arabinogalactan proteins (AGPs) are diverse along the plasmalemma of placental cells and are absent in surrounding cells in both generations. AGPs in placental cell walls may play a role in calcium binding and release associated with signal transduction as has been speculated for these glycoproteins in other plants. Callose is restricted to thin areas in cell walls of gametophyte transfer cells. In contrast to studies of transfer cells in other systems, no reaction to the JIM12 antibody against extensin was observed in Phaeoceros.

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Arabinogalactan-Proteins from the Liverwort Marchantia polymorpha L., a Member of a Basal Land Plant Lineage, Are Structurally Different to Those of Angiosperms

Cited by 22 publications

References 64 publications

Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Arabinogalactan‐like Glycoproteins from Ulva lactuca (Chlorophyta) Show Unique Features Compared to Land Plants AGPs

Cell wall polymers in the Phaeoceros placenta reflect developmental and functional differences across generations

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