Arachidonate 12-lipoxygenase of rat pineal glands: Catalytic properties and primary structure deduced from its cDNA

Hada, Takahiko; Hagiya, Hiroshi; Suzuki, Hiroshi; Arakawa, Toshiya; Nakamura, Michihiro; Matsuda, Shinji; Yoshimoto, Tanihiro; Yamamoto, Shozo; Azekawa, Takaharu; Morita, Yusuke; Ishimura, Kazunori; Kim, Hee‐Yong

doi:10.1016/0005-2760(94)90272-0

Cited by 49 publications

(25 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RNA (5 g) was incubated at 42C for 50 min in a reaction mixture of 20 l containing 100 U of reverse transcriptase (Super Script II; GIBCO BRL), 20 pmol of oligo (dT) [12][13][14][15][16][17][18] , 50 mM Tris-HCl, pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 0.5 mM dNTP, 10 mM dithiothreitol, and 10 U RNase inhibitor (Boehringer Mannheim; Mannheim, Germany). PCR primers were designed according to the reported sequence of rat pineal 12-lipoxygenase (Hada et al 1994): sense 5 Ј -AGCTGGAGCCTTTCTGACCTATTG-3 Ј and antisense 5 Ј -ACTGATTAGGGTTGGGCAGTGTAG-3 Ј . They were labeled with [ ␥ -32 P]-ATP (Amersham Pharmacia Biotech; Uppsala, Sweden) by T4 polynucleotide kinase (Toyobo; Osaka, Japan).…”

Section: Rt-pcrmentioning

confidence: 99%

“…The full-length cDNA encoding rat pineal 12-lipoxygenase (Hada et al 1994) was ligated to a eukaryotic pRC/CMV expression vector (Invitrogen; Carlsbad, CA). The expression vector was introduced by the Lipofectin method (GIBCO BRL) into ␣ TC clone 6 cells that had been established from a glucagonoma arising in transgenic mouse (Hamaguchi and Leiter 1990;Powers et al 1990).…”

Section: Expression Of 12-lipoxygenase In ␣ Tc Clone 6 Cell Linementioning

confidence: 99%

“…About 100 clones were examined by Western blotting for 12-lipoxygenase expression. Five clones were used in subsequent experiments that had shown the highest enzymatic activity as determined by a method described elsewhere (Hada et al 1994). Mocktransfected cells were established by transfection of parental plasmids.…”

Section: Expression Of 12-lipoxygenase In ␣ Tc Clone 6 Cell Linementioning

confidence: 99%

See 2 more Smart Citations

Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion

Kawajiri

Zhuang

Qiao

et al. 2000

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

There are three isoforms of arachidonate 12-lipoxygenase in mammals: platelet, leukocyte, and epidermal types. We found in this study that the leukocyte-type enzyme was present in rat pineal gland, lung, spleen, aorta, adrenal gland, spinal cord, and pancreas, as assessed by RT-PCR. Immunohistochemical analysis showed that the enzyme was localized in macrophages in lung and spleen, α-cells of pancreatic islet, zona glomerulosa cells of adrenal cortex, and neuronal cells of spinal cord and superior cervical ganglion. The presence of the 12-lipoxygenase in pancreatic α-cells was confirmed by glucagon staining in a consecutive section. We overexpressed the leukocyte-type 12-lipoxygenase cDNA in a glucagon-secreting αTC clone 6 cell line that had been established from a transgenic mouse. Glucagon secretion was stimulated by approximately twofold in the 12-lipoxygenase-expressing cells compared to the mock-transfected and original cells. The results suggest that the 12-lipoxygenase of the leukocyte type augments glucagon secretion from pancreatic islets.

show abstract

Section: Rt-pcrmentioning

confidence: 99%

Section: Expression Of 12-lipoxygenase In ␣ Tc Clone 6 Cell Linementioning

confidence: 99%

Section: Expression Of 12-lipoxygenase In ␣ Tc Clone 6 Cell Linementioning

confidence: 99%

See 1 more Smart Citation

Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion

Kawajiri

Zhuang

Qiao

et al. 2000

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Stimulatory effects of 12-lipoxygenase metabolites of arachidonic acid have been reported for various endocrine systems; LH-RH [3], melatonin [16], insulin [15], gonadotrophin [8] and aldosterone [11]. In addition, the presence of leukocytetype 12-lipoxygenase was demonstrated in various endocrine cells on biochemical, molecular biological and immunohistochemical bases: porcine anterior pituitary cells [19], rat pancreatic islet fl-cells [17], human adrenal glomerulosa cells [4] and rat pineal cells [5]. Our present work is the first immunohistochemical demonstration of a platelet-type 12-lipoxygenase present in endocrine cells.…”

Section: Discussionmentioning

confidence: 99%

“…The leukocyte-type 12-lipoxygenase has been detected not only in leukocytes but also in several nonhematopoietic tissues, including bovine trachea [6], porcine anterior pituitary gland [19], canine brain [14], rat pancreatic islets [17], human adrenal cortex [4] and rat pineal gland [5]. However, the tissue distribution of the platelet-type enzyme has not yet been investigated in detail.…”

mentioning

confidence: 99%

Platelet-type Arachidonate 12-Lipoxygenase in Mouse Gastrointestinal Tract.

Nakamura

Yamamoto

Ishimura

1998

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

Structure conservation in lipoxygenases: Structural analysis of soybean lipoxygenase-1 and modeling of human lipoxygenases

et al. 1996

View full text Add to dashboard Cite

Lipoxygenases are a class of non-heme iron dioxygenases which catalyze the hydroperoxidation of fatty acids for the biosynthesis of leukotrienes and lipoxins. The structure of the 839-residue soybean lipoxygenase-1 was used as a template to model human 5-, 12-, and 15-lipoxygenases. A distance-based algorithm for placing side chains in a low homology environment (only the four iron ligands were fixed during side chain placement) was devised. Twenty-six of the 56 conserved lipoxygenase residues were grouped in four distinct regions of the enzyme. These regions were analyzed to discern whether the side chain interactions could be duplicated in the models or whether alternate conformers should be considered. The effects of site directed mutagenesis variants were rationalized using the models of the human lipoxygenases. In particular, variants which shifted positional specificity between 12and 15-lipoxygenase activity were analyzed. Analysis of active site residues produced a model which accounts for observed lipoxygenase positional specificity and stereospecificity.

show abstract

Arachidonate 12-lipoxygenase of rat pineal glands: Catalytic properties and primary structure deduced from its cDNA

Cited by 49 publications

References 22 publications

Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion

Expression of Arachidonate 12-Lipoxygenase in Rat Tissues: A Possible Role in Glucagon Secretion

Platelet-type Arachidonate 12-Lipoxygenase in Mouse Gastrointestinal Tract.

Structure conservation in lipoxygenases: Structural analysis of soybean lipoxygenase-1 and modeling of human lipoxygenases

Contact Info

Product

Resources

About