Arachidonic acid production by Mortierella alpina with growth-coupled lipid synthesis

Ерошин, В. К.; Satroutdinov, Aidar D.; Dedyukhina, E. G.; Chistyakova, T. I.

doi:10.1016/s0032-9592(00)00151-5

Cited by 80 publications

(44 citation statements)

References 9 publications

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“…Consequently, these feedstock cannot be able to meet the amazing rising market demand for ARA. There has recently been an increased interest in using microorganisms to produce ARA (Ratledge 2004;Eroshin et al 2000). As an alternative source containing a high intracellular content of ARA, the filamentous fungus, Mortierella alpina, has been extensively studied for ARA production at various fermentation scales (Peng et al 2010;Vali et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

Ling

Zeng

Chen

et al. 2016

Bioresour. Bioprocess.

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Background:The organic nitrogen source is one of the key factors affecting Mortierella alpina cell growth as well as arachidonic acid (ARA) production. The aim of the present work is to achieve an optimized recipe of organic nitrogen source for ARA production by M. alpina by testing four organic nitrogen sources. Results:In the flasks, the results showed yeast extract or corn steep liquor were the most suitable sole nitrogen sources for biomass and ARA yield. In the bioreactor, a biomass of 17.5 g L −1 and an ARA yield of 2.7 g L −1 were achieved when using sole yeast extract, while cell autolysis was induced when using sole corn steep liquor; When using the combined nitrogen source with corn steep liquor and yeast extract at a 3:7 weight ratio, the biomass and ARA yield were significantly improved to 37 and 7.8 g L −1, respectively. Conclusions:This work evaluated whether using a mix of organic nitrogen sources could improve ARA production when scaling up from a flask to a bioreactor culture. The 3:7 ratio of corn steep liquor to yeast extract was quite favourable for large-scale ARA production, and as a result, this combination has great potential for improving fungal cultures.

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Section: Introductionmentioning

confidence: 99%

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

Ling

Zeng

Chen

et al. 2016

Bioresour. Bioprocess.

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“…As a rule, AA is absent in higher plants; the most active AA-producers were revealed among fungi belonging to the genus Mortierella (class Phycomycetes) [2][3][4][5]. It has been stated that fungi Mortierella alpina are the most attractive producers of AA (up to 70% of lipid); moreover, they are non-pathogenic and do not form mycotoxins and potentially allergenic spores under fermentation conditions [6].…”

Section: Reviewmentioning

confidence: 99%

Arachidonic acid as an elicitor of the plant defense response to phytopathogens

Dedyukhina

Kamzolova

Vainshtein

2014

Chem. Biol. Technol. Agric.

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Review summarizes both literature and own experimental data on the application of arachidonic acid (AA, C 20:4 omega-6) as an elicitor of the plant defense reactions to phytopathogens. The elicitation activity of AA was shown to depend considerably on its concentration. High AA concentrations (over 10 -5 M) induce necrosis of plant tissues and accumulation of antimicrobial compounds (phytoalexins), whereas low AA concentrations (10 -7 -10 -7 M) elicit systemic and prolonged resistance to the phytopathogen infections that is similar to immunization process. Biochemical mechanisms responsible for the elicitor activity of AA involve the whole complex of reactions including reconstruction of the cell ultrastructure, an increase in the amounts of certain enzymes and protective substances, a decrease in sterol content, redirection of isoprenoid biosynthesis from sterol derivatives toward sesquiterpenoid phytoalexins, and appearance of signal molecules, which are spread all over the plant tissues making them immunized. Field experiments revealed that the treatment of potato, tomato, sugar beet, and vine plants with low concentrations of pure AA or AA-containing preparations isolated from Phytophtora and Mortierella fungi increased plant resistance to diseases (late blight, common scrab, rhizoctonoise, cercosporose, powdery mildew, etc.) and thus enhanced the harvest yield. The application of AA-containing preparations is an alternative to the use of environmentally dangerous chemical fungicides. The processes of microbiological AA production from renewable inexpensive raw substrates are considered.

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“…It is also a direct precursor of eicosanoids, which are important modulators and mediators of a variety of essential biological processes [2]. Owing to its unique biological properties, ARA has been widely applied in medicine, pharmacology, cosmetics, food industry, agriculture, and the other fields [3].…”

Section: Introductionmentioning

confidence: 99%

Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition

Zeng

Chang

et al. 2011

Bioprocess Biosyst Eng

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To improve the arachidonic acid (ARA) accumulation in Mortierella alpina, a mixed B-group vitamin addition strategy was developed. The ARA titer reached up to 10.0 g/L, 1.7-fold of the control. At the same time, the highest specific activities of key enzymes involved in ARA biosynthesis, including malic enzyme, glucose-6-phosphate dehydrogenase and ATP: citrate lyase, were 63.3, 38.6 and 53.7% higher than the control, respectively. The possible vitamin triggered improved ARA accumulation mechanism was thus elucidated that B-group vitamins could function as the cofactors of the key enzymes involved in ARA biosynthesis, or precursors for the formation of NADPH and acetyl-CoA which were crucial for ARA synthesis, and strengthened the related metabolic flux.

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Arachidonic acid production by Mortierella alpina with growth-coupled lipid synthesis

Cited by 80 publications

References 9 publications

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

Arachidonic acid as an elicitor of the plant defense response to phytopathogens

Improving arachidonic acid accumulation in Mortierella alpina through B-group vitamin addition

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