Arachidonic acid reverses cholesterol and zinc inhibition of human voltage-gated proton channels

Han, Shuo; Applewhite, Sarah; DeCata, Jenna; Jones, Samuel; Cummings, John; Wang, Shizhen

doi:10.1016/j.jbc.2023.104918

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…It has been reported that ARA, other unsaturated fatty acids, and some of their metabolites can serve as endogenous antimicrobial compounds and their deficiency can make humans susceptible to infections with SARS-CoV-2, SARS, MERS, and other similar enveloped viruses [ 11 , 16 – 20 ]. Recent research also shows that ARA activates human voltage-gated proton channels in infected, injured, or inflamed cells [ 21 ]. Therefore, it is currently considered that the oral or intravenous administration of these fatty acids can improve recovery from these viral infections and that their presence in adequate quantities in the immunocytes and body fluids (especially in the alveolar fluid) can prevent these infections or at least reduce their severity [ 18 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level

Torrente-Rodríguez,

Ruiz-Valdepeñas Montiel,

Iftimie

et al. 2024

Microchim Acta

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A trendsetting direct competitive-based biosensing tool has been developed and implemented for the determination of the polyunsaturated fatty acid arachidonic acid (ARA), a highly significant biological regulator with decisive roles in viral infections. The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MµBs), followed by the enzymatic label of the biotin-ARA residues with streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The resulting bioconjugates were magnetically trapped onto the sensing surface of disposable screen-printed carbon transducers (SPCEs) to monitor the extent of the biorecognition reaction through amperometry. The operational functioning of the exhaustively optimized and characterized immunosensing bioplatform was highly convenient for the quantitative determination of ARA in serum samples from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2-) and respiratory syncytial virus (RSV)-infected individuals in a rapid, affordable, trustful, and sensitive manner. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level

Torrente-Rodríguez,

Ruiz-Valdepeñas Montiel,

Iftimie

et al. 2024

Microchim Acta

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Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm

Suhaiman,

Belmonte

2024

Front. Cell Dev. Biol.

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It has long been thought that exocytosis was driven exclusively by well-studied fusion proteins. Some decades ago, the role of lipids became evident and escalated interest in the field. Our laboratory chose a particular cell to face this issue: the human sperm. What makes this cell special? Sperm, as terminal cells, are characterized by their scarcity of organelles and the complete absence of transcriptional and translational activities. They are specialized for a singular membrane fusion occurrence: the exocytosis of the acrosome. This unique trait makes them invaluable for the study of exocytosis in isolation. We will discuss the lipids’ role in human sperm acrosome exocytosis from various perspectives, with a primary emphasis on our contributions to the field. Sperm cells have a unique lipid composition, very rare and not observed in many cell types, comprising a high content of plasmalogens, long-chain, and very-long-chain polyunsaturated fatty acids that are particular constituents of some sphingolipids. This review endeavors to unravel the impact of membrane lipid composition on the proper functioning of the exocytic pathway in human sperm and how this lipid dynamic influences its fertilizing capability. Evidence from our and other laboratories allowed unveiling the role and importance of multiple lipids that drive exocytosis. This review highlights the role of cholesterol, diacylglycerol, and particular phospholipids like phosphatidic acid, phosphatidylinositol 4,5-bisphosphate, and sphingolipids in driving sperm acrosome exocytosis. Furthermore, we provide a comprehensive overview of the factors and enzymes that regulate lipid turnover during the exocytic course. A more thorough grasp of the role played by lipids transferred from sperm can provide insights into certain causes of male infertility. It may lead to enhancements in diagnosing infertility and techniques like assisted reproductive technology (ART).

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Arachidonic acid reverses cholesterol and zinc inhibition of human voltage-gated proton channels

Cited by 2 publications

References 43 publications

Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level

Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level

Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm

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