Arbitrarily primed polymerase chain reaction fingerprinting and clonal analysis of oral <i>Fusobacterium nucleatum</i> isolates

George, K. S.; Reynolds, M. A.; Falkier, W. A.

doi:10.1111/j.1399-302x.1997.tb00382.x

Cited by 34 publications

(37 citation statements)

References 32 publications

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“…It provides highly reproducible restriction profiles with distinct fragments representing the entire bacterial chromosome. 45 PFGE has been used previously to match bacteraemias to a source such as healthcare workers' hands 46 and oral organisms involved in bacteraemia have also been matched to their site of origin. 47 In the present study, only two aerobic organisms were shown to have the same genotype in root canal and blood samples.…”

Section: Discussionmentioning

confidence: 99%

Detection of bacteraemias during non-surgicalroot canal treatment

Savarrio

MacKenzie

Riggio

et al. 2005

Journal of Dentistry

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Section: Discussionmentioning

confidence: 99%

Detection of bacteraemias during non-surgicalroot canal treatment

Savarrio

MacKenzie

Riggio

et al. 2005

Journal of Dentistry

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“…The fourth was the presence on both the BBHK agar and the fusobacteriaselective medium of F. necrophorum, which has never before been observed in our laboratory after culturing oral samples for Fusobacterium species for more than 20 years. [18][19][20][21][22][23][24][25] Another interesting observation and a major difference from the ANG samples, which we previously cultured in Nigeria, was the absence of F. nucleatum colonies and its replacement with F. necrophorum.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Falkler¹,

Enwonwu²,

Idigbe³

1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. A study of the predominant microflora in active sites of noma (cancrum oris) lesions was carried out in eight noma patients 3-15 years of age in Sokoto State in northwestern Nigeria. Paper point sampling and conventional anaerobic microbiologic techniques were used. Fusobacterium necrophorum was recovered from 87.5% of the noma lesions. Oral microorganisms included Prevotella intermedia, alpha-hemolytic streptococci, and Actinomyces spp. which were isolated from 75.0%, 50.0%, and 37.5% of the patients, respectively. Peptostreptococcus micros, Veillonella parvula, Staphylococcus aureus, and Pseudomonas spp. were each recovered from one lesion. The F. necrophorum and P. intermedia isolates were tested for antibiotic sensitivity to clindamycin, tetracycline, metronidazole, and penicillin using the E-test, and all strains were observed to be sensitive to all of the antibiotics tested with the exception of one strain of P. intermedia, which showed resistance to penicillin. The first reported isolation from human noma lesions of F. necrophorum, a pathogen primarily associated with animal diseases, may have important etiologic and animal transmission implications.Noma (cancrum oris) is a destructive gangrenous stomatitis occurring mainly in children. It may lead to devastating facial deformity, circumferential scarring, stenosis of the mouth, and in many cases death. This disease occurs almost exclusively among poor malnourished children in developing countries.

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“…In contrast, the diversity of S. sanguinis (31) and the mutans streptococi (S. mutans and S. sobrinus) appears to be limited (1,5,20,28,34,38). Among gram-negative commensal oral bacteria, studies of Eikenella corrodens (7,8,18), Fusobacterium nucleatum (19,42), and Prevotella melaninogenica (27) show that individuals may harbor multiple genotypes whereas, in contrast, colonization with Porphyromonas gingivalis (43) and Actinobacillus actinomycetemcomitans (13,21) appears to be monoclonal.…”

Section: Discussionmentioning

confidence: 99%

Clonal Diversity and Turnover of Streptococcus mitis bv. 1 on Shedding and Nonshedding Oral Surfaces of Human Infants during the First Year of Life

Kirchherr

Bowden

Richmond

et al. 2005

Clin Vaccine Immunol

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Streptococcus mitis bv. 1 is a pioneer colonizer of the human oral cavity. Studies of its population dynamics within parents and their infants and within neonates have shown extensive diversity within and between subjects. We examined the genetic diversity and clonal turnover of S. mitis bv. 1 isolated from the cheeks, tongue, and primary incisors of four infants from birth to 1 year of age. In addition, we compared the clonotypes of S. mitis bv. 1 isolated from their mothers' saliva collected in parallel to determine whether the mother was the origin of the clones colonizing her infant. Of 859 isolates obtained from the infants, 568 were unique clones. Each of the surfaces examined, whether shedding or nonshedding, displayed the same degree of diversity. Among the four infants it was rare to detect the same clone colonizing more than one surface at a given visit. There was little evidence for persistence of clones, but when clones were isolated on multiple visits they were not always found on the same surface. A similar degree of clonal diversity of S. mitis bv. 1 was observed in the mothers' saliva as in their infants' mouths. Clones common to both infant and mothers' saliva were found infrequently suggesting that this is not the origin of the infants' clones. It is unclear whether mucosal immunity exerts the environmental pressure driving the genetic diversity and clonal turnover of S. mitis bv. 1, which may be mechanisms employed by this bacterium to evade immune elimination.Streptococcus mitis bv. 1, S. oralis and S. salivarius are species of viridans streptococci that are pioneer colonizers of the human oral cavity and remain numerically significant throughout life (17,23,32). However, the origin of these bacteria remains to be determined. Despite the abundance of commensal bacteria present in the birth canal, none of these are able to successfully colonize the mouth of the infant suggesting that they do not have tropism for the oropharyngeal mucosa. It has been proposed that commensal bacteria are transferred from the primary care-giver (27,29,33,42), external environment (6), and from other areas of the respiratory tract (22,23).Successful colonization depends on the ability of the bacteria to circumvent host innate and acquired immunity in order that they can adhere to oral surfaces and avoid removal via the flushing action of saliva and mastication. Neonatal saliva has been shown to contain secretory immunoglobulin A (SIgA) antibodies that react with these bacteria (9, 10) but these antibodies appear insufficient to completely block adherence and subsequent colonization. Several species of viridans streptococci including the pioneers, S. mitis bv. 1 and S. oralis, produce IgA1 protease (11, 26) which may inactivate SIgA1 antibodies in saliva. In this context, it is interesting that over 90% of SIgA in the saliva of neonates belongs to subclass 1 (16). Furthermore, viridans streptococci elaborate extracellular polysaccharide (4) and bind salivary macromolecules (40) which may mask them from host immunit...

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Arbitrarily primed polymerase chain reaction fingerprinting and clonal analysis of oral Fusobacterium nucleatum isolates

Cited by 34 publications

References 32 publications

Detection of bacteraemias during non-surgicalroot canal treatment

Detection of bacteraemias during non-surgicalroot canal treatment

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Clonal Diversity and Turnover of Streptococcus mitis bv. 1 on Shedding and Nonshedding Oral Surfaces of Human Infants during the First Year of Life

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