The data on the number of species of insects associated with various trees in Britain have been reanalyzed to factor out possible bias from phylogenetic effects. It was found that tree availability (range and abundance) continues to provide a good predictor (r ؍ 0.852) of insect-species richness, slightly better than straightforward cross-species analyses. Of the two components of tree availability, tree abundance gives a much better prediction than tree range. The species richness on trees of major taxa with similar trophic habits (Lepidoptera and Hymenoptera͞Symphyta and the two suborders of the Homoptera-Auchenorrhyncha and Sternorrhyncha) shows positive correlations; there is thus no evidence of competitive exclusion at this taxonomic level.The determinants of diversity are of central issue in ecology and have gained particular urgency with the escalating loss of species and habitats in recent years. Hence, the relationship of resource availability and its constituent components to species richness is a focus of long-term and continuing attention. Recently, it has been recognized that the sorts of multispecific data sets used to examine questions such as this one can be unwittingly biased because of phylogenetic relatedness among the included species (1, 2). Several reanalyses of data thought to support some ''classic'' ecological principles have shown that when phylogenetic relatedness is appropriately taken into account, the relationship may not, after all, be upheld (e.g., refs. 3 and 4). The relationship between the species richness of the insect herbivore community and the abundance of the tree species is commonly cited as one of the definitive indications of the relationship between resource availability and species richness (4-7). Here, we present a reanalysis of these data, taking into account the potentially confounding effect of phylogenetic relatedness.Among other things, previous analyses established that the total number of phytophagous insect species to be found on a particular tree species correlated with a measure of the availability of that tree species (5-7). Breaking down insect species into subgroups, each containing similarly feeding, taxonomically related taxa, indicated that the relationship with tree-species availability operated at that level as well. Further, taxonomic isolation of a tree species, that is, whether a tree species has few or many close relatives in Great Britain, was found to explain a small but significant proportion of the variation in insect-species richness on the trees in their data set: the more taxonomically isolated tree species supported fewer insect species than otherwise expected. Subsequent studies have applied these findings and this methodology (i) to compare insect-species richness and host-species availability in other geographic areas and taxa (e.g., 8-11), in terms of parasitoid-host, predator-prey, and ectomycorrhizal fungihost tree relationships (e.g., refs. 12-15); (ii) to infer causes behind the patterns of diversity in urban areas, pin...