Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system

Metz, Stefan; Pijlman, Gorben P.

doi:10.1016/j.jip.2011.05.002

Cited by 56 publications

(54 citation statements)

References 169 publications

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“…Arboviruses take a unique position within the virosphere by displaying active replication in both vertebrate hosts (humans, other mammals, and birds) and invertebrate vectors (e.g., mosquitoes, ticks, midges, and sandflies) (41). Upon infection of mosquitoes, the virus persistently replicates in multiple tissues of the insect, and high virus titers accumulate in the salivary glands by the end of this so-called extrinsic incubation time, typically 1 to 2 weeks.…”

mentioning

confidence: 99%

Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Schnettler

Sterken

Leung

et al. 2012

J Virol

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277

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mentioning

confidence: 99%

Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Schnettler

Sterken

Leung

et al. 2012

J Virol

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264

277

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“…Also, vaccines targeting highly pathogenic viruses that are transmitted by arthropod vectors (arboviruses) are being developed (Metz & Pijlman, 2011). Characteristics of baculovirus-based human vaccines that are currently approved or are in later phases of clinical trials are given in Table 2.…”

Section: Baculoviruses and Vaccine Developmentmentioning

confidence: 99%

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

Lapointe¹,

Thumbi²,

Lucarotti³

2012

Integrated Pest Management and Pest Control - Current and Future Tactics

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“…BTV is primarily transmitted by biting midges (Culicoides species) and like other vector-borne viruses is difficult to control using conventional biosecurity measures (1,2). Therefore, vaccination campaigns are important tools to prevent virus spread and clinical BT disease (3).…”

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confidence: 99%

Purification, Stability, and Immunogenicity Analyses of Five Bluetongue Virus Proteins for Use in Development of a Subunit Vaccine That Allows Differentiation of Infected from Vaccinated Animals

Anderson

Bréard

Bengtsson

et al. 2014

Clin. Vaccine Immunol.

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e Bluetongue virus (BTV) causes bluetongue disease, a vector-borne disease of ruminants. The recent northerly spread of BTV serotype 8 in Europe resulted in outbreaks characterized by clinical signs in cattle, including unusual teratogenic effects. Vaccination has been shown to be crucial for controlling the spread of vector-borne diseases such as BTV. With the aim of developing a novel subunit vaccine targeting BTV-8 that allows differentiation of infected from vaccinated animals, five His-tagged recombinant proteins, VP2 and VP5 of BTV-8 and NS1, NS2, and NS3 of BTV-2, were expressed in baculovirus or Escherichia coli expression systems for further study. Optimized purification protocols were determined for VP2, NS1, NS2, and NS3, which remained stable for detection for at least 560 to 610 days of storage at ؉4°C or ؊80°C, and Western blotting using sera from vaccinated or experimentally infected cattle indicated that VP2 and NS2 were recognized by BTV-specific antibodies. To characterize murine immune responses to the four proteins, mice were subcutaneously immunized twice at a 4-week interval with one of three protein combinations plus immunostimulating complex ISCOM-Matrix adjuvant or with ISCOM-Matrix alone (n ‫؍‬ 6 per group). Significantly higher serum IgG antibody titers specific for VP2 and NS2 were detected in immunized mice than were detected in controls. VP2, NS1, and NS2 but not NS3 induced specific lymphocyte proliferative responses upon restimulation of spleen cells from immunized mice. The data suggest that these recombinant purified proteins, VP2, NS1, and NS2, could be an important part of a novel vaccine design against BTV-8.

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Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system

Cited by 56 publications

References 169 publications

Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

Purification, Stability, and Immunogenicity Analyses of Five Bluetongue Virus Proteins for Use in Development of a Subunit Vaccine That Allows Differentiation of Infected from Vaccinated Animals

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