Arbutin ameliorates hyperglycemia, dyslipidemia and oxidative stress and modulates adipocytokines and PPARγ in high-fat diet/streptozotocin-induced diabetic rats

Abduh, Maisa Siddiq; Alzoghaibi, Mohammed A; Alzoghaibi, Abdullah M.; Bin-Ammar, Albandari; Alotaibi, Mohammed; Kamel, Emadeldin M.; Mahmoud, Ayman M.

doi:10.1016/j.lfs.2023.121612

Cited by 10 publications

(6 citation statements)

References 73 publications

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“…Here, HFD/STZ-challenged animals showed hyperglycemia marked by glucose intolerance and IR. The developed T2D was consistent with our previous investigations, showing IR and hyperglycemia in HFD/STZinduced rats (Mahmoud et al, 2012;Germoush et al, 2019;Elsayed et al, 2020;Abduh et al, 2023). The chronic hyperglycemia in this model was supported by the values of HbA1c%, a reliable marker for both diagnosis and prognosis of DM (American Diabetes Association, 2014) reported in our recent work (Abduh et al, 2023).…”

Section: Discussionsupporting

confidence: 91%

“…The developed T2D was consistent with our previous investigations, showing IR and hyperglycemia in HFD/STZinduced rats (Mahmoud et al, 2012;Germoush et al, 2019;Elsayed et al, 2020;Abduh et al, 2023). The chronic hyperglycemia in this model was supported by the values of HbA1c%, a reliable marker for both diagnosis and prognosis of DM (American Diabetes Association, 2014) reported in our recent work (Abduh et al, 2023). Hyperglycemia was associated with hypoinsulinemia, and the development of IR as the value of HOMA-IR was revealed.…”

Section: Discussionsupporting

confidence: 91%

“…Hyperglycemia was associated with hypoinsulinemia, and the development of IR as the value of HOMA-IR was revealed. Similar to these findings, elevated glucose, HbA1c%, and HOMA-IR along with decreased insulin were reported in HFD/STZ-challenged rats (Abduh et al, 2023).…”

Section: Discussionsupporting

confidence: 86%

“…The binding of E. peplus compounds with hexokinase II (PDB ID: 2NZT), NF-κB-DNA complex (PDB ID 1LE9), and PPARγ (PDB ID: 2PRG) was investigated as previously reported (Supplementary Material) (Sami et al, 2022;Abduh et al, 2023).…”

Section: In Silico Molecular Dockingmentioning

confidence: 99%

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A flavonoid-rich fraction of Euphorbia peplus attenuates hyperglycemia, insulin resistance, and oxidative stress in a type 2 diabetes rat model

et al. 2023

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Background: Type 2 diabetes (T2D) is a metabolic disorder characterized by insulin resistance (IR) and hyperglycemia. Plants are valuable sources of therapeutic agents for the management of T2D. Euphorbia peplus has been widely used as a traditional medicine for the treatment of various diseases, but its beneficial role in T2D has not been fully explored.Methods: The anti-diabetic efficacy of E. peplus extract (EPE) was studied using rats with T2D induced by high-fat diet (HFD) and streptozotocin (STZ). The diabetic rats received 100, 200, and 400 mg/kg EPE for 4 weeks.Results: Phytochemical fractionation of the aerial parts of E. peplus led to the isolation of seven known flavonoids. Rats with T2D exhibited IR, impaired glucose tolerance, decreased liver hexokinase and glycogen, and upregulated glycogen phosphorylase, glucose-6-phosphatase (G-6-Pase), and fructose-1,6-bisphosphatase (F-1,6-BPase). Treatment with 100, 200, and 400 mg/kg EPE for 4 weeks ameliorated hyperglycemia, IR, liver glycogen, and the activities of carbohydrate-metabolizing enzymes. EPE attenuated dyslipidemia, serum transaminases, tumor necrosis factor (TNF)-α, interleukin (IL)-1β and liver lipid accumulation, nuclear factor (NF)-κB p65, and lipid peroxidation, nitric oxide and enhanced antioxidants. All EPE doses upregulated serum adiponectin and liver peroxisome proliferator-activated receptor γ (PPARγ) in HFD/STZ-induced rats. The isolated flavonoids showed in silico binding affinity toward hexokinase, NF-κB, and PPARγ.Conclusion:E. peplus is rich in flavonoids, and its extract ameliorated IR, hyperglycemia, dyslipidemia, inflammation and redox imbalance, and upregulated adiponectin and PPARγ in rats with T2D.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 86%

Section: In Silico Molecular Dockingmentioning

confidence: 99%

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A flavonoid-rich fraction of Euphorbia peplus attenuates hyperglycemia, insulin resistance, and oxidative stress in a type 2 diabetes rat model

et al. 2023

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“…Multiple studies have shown that PPARγ regulates downstream genes, reduces the accumulation of triglycerides in cells, increases cholesterol efflux from liver tissue, and prevents steatosis (Chyau et al., 2020 ; Li et al., 2023 ). In addition, PPAR γ also regulated inflammatory factors in various ways to suppress inflammation in NAFLD, which included terminating NF‐ κB P65 transcription and downregulating IL‐6, TNF‐α, IL‐4, IL‐10, and IL‐2 inflammatory factors to improve inflammation (Abduh et al., 2023 ; Gross et al., 2017 ). Our data suggested that the GT + EX combined intervention for 22 weeks inhibited the activation of NF‐κB pathway, decreased the expression of proinflammatory cytokines, and consequently improved hepatic statuses.…”

Section: Discussionmentioning

confidence: 99%

Long‐term drinking of green tea combined with exercise improves hepatic steatosis and obesity in male mice induced by high‐fat diet

Wang,

Gu,

Zhang

et al. 2023

Food Science & Nutrition

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Dietary habits and exercise play an important role in the well‐being of human health. Currently, how long of drinking tea combined with exercise could efficiently ameliorate hepatic steatosis and obesity still needs to be investigated. Here, short‐term and long‐term green tea drinking combined with exercise were studied to improve hepatic steatosis and obesity in high‐fat diet‐induced (HF) mice. Our results showed that Yunkang 10 green tea (GT) combined with exercise (Ex) exhibited synergistic prevention effects on ameliorating hepatic steatosis and obesity. Especially, 22‐week intervention with GT or Ex improved all symptoms of obesity, which indicated that long‐term intervention exhibited profound preventive effects than the short term. Moreover, the combined intervention of 22 weeks inhibited the activation of NF‐κB pathway and the expression of proinflammatory cytokines, which suggests that tea combined exercise may improve liver steatosis mainly by inhibiting inflammation. The key molecules for regulating lipid and glucose metabolism SCD1 were obviously downregulated, and GLU2 and PPARγ were significantly upregulated by GT and exercise in the liver of high‐fat diet‐induced mice. This study demonstrated that long‐term intervention with GT and exercise effectively relieved hepatic steatosis and obesity complications by ameliorating hepatic inflammation, reducing lipid synthesis, and accelerating glucose transport.

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Phytochemical Analysis, and Antioxidant and Hepatoprotective Activities of Chamaerops humilis L. Leaves; A Focus on Xanthine Oxidase

Ahmed,

Kamel,

Mahmoud

et al. 2024

Chemistry & Biodiversity

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Chamaerops humilis L. is clumping palm of the family Arecaceae with promising health‐promoting effects. Parts of this species are utilized as food and employed in folk medicine to treat several disorders. This study investigated the phytochemical constituents of C. humilis leaves and their antioxidant and xanthine (XO) inhibitory activities in vitro and in acetaminophen (APAP)‐induced hepatotoxicity in rats. Eleven compounds were isolated from C. humilis ethanolic extract (CHEE). CHEE and the butanol, n‐hexane, and dichloromethane fractions exhibited in vitro radical scavenging and XO inhibitory efficacy. The computational findings revealed the tendency of the isolated compounds towards the active site of XO. In vivo, CHEE ameliorated liver function markers (ALT, AST, ALP, and albumin) and prevented tissue injury induced by APAP in rats. CHEE suppressed hepatic XO, decreased serum uric acid and liver MDA, and enhanced GSH, SOD, and catalase in APAP‐treated rats. CHEE ameliorated serum TNF‐α and IL‐1β in APAP‐treated rats. Thus, C. humilis is rich in beneficial phytochemicals that possess binding affinity towards XO. C. humilis exhibited potent in vitro antioxidant and XO inhibitory activities, and prevented APAP hepatotoxicity by attenuating tissue injury, oxidative stress and inflammation.

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Arbutin ameliorates hyperglycemia, dyslipidemia and oxidative stress and modulates adipocytokines and PPARγ in high-fat diet/streptozotocin-induced diabetic rats

Cited by 10 publications

References 73 publications

A flavonoid-rich fraction of Euphorbia peplus attenuates hyperglycemia, insulin resistance, and oxidative stress in a type 2 diabetes rat model

A flavonoid-rich fraction of Euphorbia peplus attenuates hyperglycemia, insulin resistance, and oxidative stress in a type 2 diabetes rat model

Long‐term drinking of green tea combined with exercise improves hepatic steatosis and obesity in male mice induced by high‐fat diet

Phytochemical Analysis, and Antioxidant and Hepatoprotective Activities of Chamaerops humilis L. Leaves; A Focus on Xanthine Oxidase

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