Arcanobacterium buesumense sp. nov., isolated from an anal swab of a male harbour seal (Phoca vitulina)

Abstract

A polyphasic taxonomic study was performed on an unidentified previously described Arcanobacterium -like Gram-positive strain 2701T isolated from an anal swab of a dead male harbour seal. Comparative 16S rRNA sequencing showed that the bacterium belonged to the genus Arcanobacterium in the family Arcanobacteriaceae . The genome sequence of the strain was obtained by Borowiak et al. []. The genome had a G+C content of 49 mol% and a total length of 1.94 Mb. The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolate with the genus Arcanobacterium . The polar lipid profile consisted of diphosphatidylglycerol and an unidentified phospholipid as major components and two unidentified lipids, a further unidentified phospholipid, two unidentified phosphoglycolipids as well as phosphatidylglycerol. The major fatty acids were C16 : 0, C18 : 1 and C18 : 0. Biochemical and phylogenetic analyses clearly distinguished the isolate from other members of the genus Arcanobacterium and closely related other species. Based on these results, it is proposed that the unknown Arcanobacterium sp. strain 2701T should be classified as representing a novel species with the name Arcanobacterium buesumense sp. nov. The type strain is 2701T (=DSM 112952T=LMG 32446T).

Arcanobacterium pinnipediorum Strain DSM 28752 Isolated from a Harbour Seal: Complete Genome Sequence

“…In harbor seals and other marine mammals, Arcanobacterium phocae is widespread and sometimes associated with wound infections ( 6 , 7 ). Routine microbial diagnostic investigations performed as part of a program monitoring harbor seals in the German North Sea revealed that harbor seals also appear to be a natural reservoir for other Arcanobacterium species, as three novel species were identified—namely, Arcanobacterium phocisimile ( 8 , 9 ), A. buesumense ( 10 , 11 ), and A. pinnipediorum ( 1 , 12 ).…”