Archaeal and bacterial diversity in five different hydrothermal ponds in the Copahue region in Argentina

Urbieta, María Sofía; Toril, Elena González; Giaveno, María Alejandra; Aguilera, Ángeles; Donati, Edgardo R.

doi:10.1016/j.syapm.2014.05.012

Cited by 40 publications

(40 citation statements)

References 49 publications

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“…Conversely, It3 was mainly populated by Archaea (96.6%), including species such as Acidianus, Sulfolobus and Pyrobaculum (Menzel et al 2015). When these data were compared with those from previous studies (Inskeep et al 2013a;Sahm et al 2013;Urbieta et al 2014;Wemheuer et al 2013) the authors conclude that environmental chemico-physical parameters are the major determinants in shaping the structure and composition of the microbial community (Menzel et al 2015).…”

Section: Phlegraean Fields Italymentioning

confidence: 82%

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

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Geothermally heated regions of Earth, such as terrestrial volcanic areas (fumaroles, hot springs, and geysers) and deep-sea hydrothermal vents, represent a variety of different environments populated by extremophilic archaeal and bacterial microorganisms. Since most of these microbes thriving in such harsh biotopes, they are often recalcitrant to cultivation; therefore, ecological, physiological and phylogenetic studies of these microbial populations have been hampered for a long time. More recently, culture-independent methodologies coupled with the fast development of next generation sequencing technologies as well as with the continuous advances in computational biology, have allowed the production of large amounts of metagenomic data. Specifically, these approaches have assessed the phylogenetic composition and functional potential of microbial consortia thriving within these habitats, shedding light on how extreme physico-chemical conditions and biological interactions have shaped such microbial communities. Metagenomics allowed to better understand that the exposure to an extreme range of selective pressures in such severe environments, accounts for genomic flexibility and metabolic versatility of microbial and viral communities, and makes extreme-and hyper-thermophiles suitable for bioprospecting purposes, representing an interesting source for novel thermostable proteins that can be potentially used in several industrial processes.Keywords Hyperthermophiles Á Geothermal environments Á Microbial and viral metagenomics Á CRISPR Á Enzyme discovery BackgroundHot springs populated by extreme thermophiles (T opt : 65-79°C) and hyperthermophiles (T opt [ 80°C) (DeCastro et al. 2016) are very diverse and some of them show combinations of extreme chemo-physical conditions, such as temperature, acidic or alkaline pHs, high pressure, and high concentrations of salts and heavy metals (López-López et al. 2013). As with all studies of environmental microbiology, our understanding of the composition, functional and physiological dynamics, and evolution of extreme-and hyper-thermophilic microbial consortia has lagged A. Strazzulli Á M. Moracci Á P. Contursi

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Section: Phlegraean Fields Italymentioning

confidence: 82%

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

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“…This result is consistent with our previous shotgun metagenome analyses for SK (Chan et al, 2015) and US (data not shown). High-temperature environments were previously generally believed to be the realm of archaea (Urbieta et al, 2014; Li et al, 2015). However, recent studies applying molecular methods have revealed that bacteria rather are the predominant prokaryotic communities in such environments (Badhai et al, 2015; López-López et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of Physiochemical Factors on Prokaryotic Biodiversity in Malaysian Circumneutral Hot Springs

Chan

et al. 2017

Front. Microbiol.

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Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3–V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334–26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.

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“…Our research group has worked on the microbiology of Copahue, focusing on prokaryotic biodiversity assessments through culture-independent approaches using microbial ecology techniques such as amplification and sequencing of the complete 16S rRNA gene of the entire community and FISH (Fluorescence In Situ Hybridisation) or its more sensible version CARD FISH (Catalysed Reported Deposition Fluorescence In Situ Hybridisation) for quantitative information on the species distribution and community structure [ 21 , 22 , 23 ]. Additionally, we tried to isolate and characterise autochthonous extremophilic microorganisms [ 24 , 25 , 26 ].…”

Section: Description Of the Copahue Geothermal Systemmentioning

confidence: 99%

“…We have proposed the study of prokaryotic biodiversity in the Copahue geothermal field through two complementary strategies. On the one hand, we performed comprehensive non-culture based studies on the whole prokaryotic community of different ponds that were selected to represent the diverse temperature and pH characteristics found in the area ( Table 1 ) as well as the influence of anthropogenic intervention [ 22 ]. The assessments of water samples as well as the microbial biofilms that develop at the edges of the rocks where thermal activity is less intense were conducted by amplification and sequencing of the partial 16S rRNA gene of bacteria and archaea (the set of primers used was 8F: 5′-AGAGTTTGATC(A/C)TGGC-3′ for Bacteria and 25F: 5′-TCYGGTTGATCCYGCCRG-3′ for Archaea ; reverse primer for both was 1492r: 5′-TACCTTGTTACGACTT-3′ [ 27 , 28 ]).…”

Section: Prokaryotic Biodiversity At Copahue Geothermal Fieldmentioning

confidence: 99%

Copahue Geothermal System: A Volcanic Environment with Rich Extreme Prokaryotic Biodiversity

et al. 2015

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The Copahue geothermal system is a natural extreme environment located at the northern end of the Cordillera de los Andes in Neuquén province in Argentina. The geochemistry and consequently the biodiversity of the area are dominated by the activity of the Copahue volcano. The main characteristic of Copahue is the extreme acidity of its aquatic environments; ponds and hot springs of moderate and high temperature as well as Río Agrio. In spite of being an apparently hostile location, the prokaryotic biodiversity detected by molecular ecology techniques as well as cultivation shows a rich and diverse environment dominated by acidophilic, sulphur oxidising bacteria or archaea, depending on the conditions of the particular niche studied. In microbial biofilms, found in the borders of the ponds where thermal activity is less intense, the species found are completely different, with a high presence of cyanobacteria and other photosynthetic species. Our results, collected during more than 10 years of work in Copahue, have enabled us to outline geomicrobiological models for the different environments found in the ponds and Río Agrio. Besides, Copahue seems to be the habitat of novel, not yet characterised autochthonous species, especially in the domain Archaea.

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Archaeal and bacterial diversity in five different hydrothermal ponds in the Copahue region in Argentina

Cited by 40 publications

References 49 publications

Metagenomics of microbial and viral life in terrestrial geothermal environments

Metagenomics of microbial and viral life in terrestrial geothermal environments

Effects of Physiochemical Factors on Prokaryotic Biodiversity in Malaysian Circumneutral Hot Springs

Copahue Geothermal System: A Volcanic Environment with Rich Extreme Prokaryotic Biodiversity

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