Archaeal microbiota population in piglet feces shifts in response to weaning: Methanobrevibacter smithii is replaced with Methanobrevibacter boviskoreani

Federici, Sara; Miragoli, Francesco; Pisacane, Vincenza; Rebecchi, Annalisa; Morelli, L.; Callegari, Maria Luisa

doi:10.1093/femsle/fnv064

Cited by 21 publications

(22 citation statements)

References 35 publications

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“…The CON14 group exhibited an enrichment of an OTU related to Methanobrevibacter boviskoreani and this OTU and another related OTU increased in LD18 and HD18 samples. Methanobrevibacter boviskoreani is also a hydrogenotrophic methanogen which is able to replace Methanobrevibacter smithii in weaning pigs ( Lee et al, 2013 ; Federici et al, 2015 ). In contrast, Mootral reduced the abundance of several OTUs from genus Methanobrevibacter in the LD14, LD18, and HD18 treatment.…”

Section: Discussionmentioning

confidence: 99%

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Eger

Graz²,

Riede

et al. 2018

Front. Microbiol.

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The reduction of methane emissions by ruminants is a highly desirable goal to mitigate greenhouse gas emissions. Various feed additives have already been tested for their ability to decrease methane production; however, practical use is often limited due to negative effects on rumen fermentation or high costs. Organosulphur compounds from garlic (Allium sativum) and flavonoids have been identified as promising plant-derived compounds which are able to reduce methane production. Here, we evaluated the effects of a combination of garlic powder and bitter orange (Citrus aurantium) extracts, Mootral, on ruminal methane production, ruminal fermentation and the community of methanogenic Archaea by using the rumen simulation technique as ex vivo model. The experiment consisted of an equilibration period of 7 days, an experimental period of 8 days and a withdrawal period of 4 days. During the experimental period three fermenters each were either treated as controls (CON), received a low dose of Mootral (LD), a high dose of Mootral (HD), or monensin (MON) as positive control. Application of Mootral strongly reduced the proportion of methane in the fermentation gas and the production rate of methane. Moreover, the experimental mixture induced a dose-dependent increase in the production rate of short chain fatty acids and in the molar proportion of butyrate. Some effects persisted during the withdrawal period. Both, single strand conformation polymorphism and Illumina MiSeq 16S rRNA amplicon sequencing indicated an archaeal community distinct from CON and MON samples in the LD and HD samples. Among archaeal families the percentage of Methanobacteriaceae was reduced during application of both doses of Mootral. Moreover, several significant differences were observed on OTU level among treatment groups and after withdrawal of the additives for LD and HD group. At day 14, 4 OTUs were positively correlated with methane production. In conclusion this mixture of garlic and citrus compounds appears to effectively reduce methane production by alteration of the archaeal community without exhibiting negative side effects on rumen fermentation.

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Section: Discussionmentioning

confidence: 99%

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Eger

Graz²,

Riede

et al. 2018

Front. Microbiol.

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“…It is noteworthy that all three sequenced strains of the Mbb. wolinii clade harbor the genes for ethanol utilization, which could point to a specific ecological role of the species in this clade; however, there are only few studies that have detected significant numbers of either of these three species in a natural environment [5, 60] making it currently difficult to determine potential cooccurrences or specific syntrophic interactions with other microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Analysis of Members of the GeneraMethanosphaeraandMethanobrevibacterReveals Distinct Clades with Specific Potential Metabolic Functions

Schneider

Soh

Daniel

et al. 2018

Archaea

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Methanobrevibacter and Methanosphaera species represent some of the most prevalent methanogenic archaea in the gastrointestinal tract of animals and humans and play an important role in this environment. The aim of this study was to identify genomic features that are shared or specific for members of each genus with a special emphasis of the analysis on the assimilation of nitrogen and acetate and the utilization of methanol and ethanol for methanogenesis. Here, draft genome sequences of Methanobrevibacter thaueri strain DSM 11995T, Methanobrevibacter woesei strain DSM 11979T, and Methanosphaera cuniculi strain 4103T are reported and compared to those of 16 other Methanobrevibacter and Methanosphaera genomes, including genomes of the 13 currently available types of strains of the two genera. The comparative genome analyses indicate that among other genes, the absence of molybdopterin cofactor biosynthesis is conserved in Methanosphaera species but reveals also that the three species share a core set of more than 300 genes that distinguishes the genus Methanosphaera from the genus Methanobrevibacter. Multilocus sequence analysis shows that the genus Methanobrevibacter can be subdivided into clades, potentially new genera, which may display characteristic specific metabolic features. These features include not only the potential ability of nitrogen fixation and acetate assimilation in a clade comprised of Methanobrevibacter species from the termite gut and Methanobrevibacter arboriphilus strains but also the potential capability to utilize ethanol and methanol in a clade comprising Methanobrevibacter wolinii strain DSM 11976T, Mbb. sp. AbM4, and Mbb. boviskoreani strain DSM 25824T.

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“…2006; Federici et al. 2015). Unfortunately, PCR amplification of methanogenic 16S rRNA genes resulted in bands that were all identified as Methanobrevibacter smithii , while for SRB no amplicons were obtained from fecal DNA of patients with CF.…”

Section: Methodsmentioning

confidence: 99%

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Miragoli

Federici

Ferrari

et al. 2016

FEMS Microbiology Ecology

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Cystic fibrosis is often associated with intestinal inflammation due to several factors, including altered gut microbiota composition. In this study, we analyzed the fecal microbiota among patients with cystic fibrosis of 10–22 years of age, and compared the findings with age-matched healthy subjects. The participating patients included 14 homozygotes and 14 heterozygotes with the delF508 mutation, and 2 heterozygotes presenting non-delF508 mutations. We used PCR-DGGE and qPCR to analyze the presence of bacteria, archaea and sulfate-reducing bacteria. Overall, our findings confirmed disruption of the cystic fibrosis gut microbiota. Principal component analysis of the qPCR data revealed no differences between homozygotes and heterozygotes, while both groups were distinct from healthy subjects who showed higher biodiversity. Archaea were under the detection limit in all homozygotes subjects, whereas methanogens were detected in 62% of both cystic fibrosis heterozygotes and healthy subjects. Our qPCR results revealed a low frequency of sulfate-reducing bacteria in the homozygote (13%) and heterozygote (13%) patients with cystic fibrosis compared with healthy subjects (87.5%). This is a pioneer study showing that patients with cystic fibrosis exhibit significant reduction of H2-consuming microorganisms, which could increase hydrogen accumulation in the colon and the expulsion of this gas through non-microbial routes.

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Archaeal microbiota population in piglet feces shifts in response to weaning: Methanobrevibacter smithii is replaced with Methanobrevibacter boviskoreani

Cited by 21 publications

References 35 publications

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Comparative Genomic Analysis of Members of the GeneraMethanosphaeraandMethanobrevibacterReveals Distinct Clades with Specific Potential Metabolic Functions

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

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