Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids

Abstract

A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75 °C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H2 and CO2 using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3–94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1T (=DSM 19444T=JCM 14716T).

Isolation of Sulfate-Reducing Bacteria from Sediments Above the Deep-Subseafloor Aquifer

“…The assumption that autotrophy is also a common metabolic attribute within the crust at IODP Site U1301, is supported by the isolation of a novel member of the genus Archaeoglobus from a fluid-influenced sample of ODP Site 1226 (Steinsbu et al, 2010). Archaeoglobus sulfaticallidus sp.…”

A Post-Genomic View of the Ecophysiology, Catabolism and Biotechnological Relevance of Sulphate-Reducing Prokaryotes

“…This metabolic trait is apparently not rare, as several novel (strictly) chemolithoautotrophic (with H 2 and CO 2 ) isolates have been reported from deep-sea vent systems, including Thermodesulfobacterium hydrogeniphilum SL6 T originating from hydrothermal vent at Guaymas Basin ( Jeanthon et al, 2002), Desulfurobacterium pacificum SL17 T and Desulfurobacterium atlanticum SL22 T from hydrothermal vent chimney at the East Pacific Rise and Mid-Atlantic Ridge, respectively (L'Haridon et al, 2006), Thermodesulfatator indicus CIR29812 T from the Kairei vent field on the Central Indian Ridge (Moussard et al, 2004), Thermodesulfatator atlanticus AT1325 T from the Rainbow vent field on the Mid-Atlantic Ridge (Alain et al, 2010) and Archaeoglobus sulfaticallidus PM70-1 T isolated from black rust retrieved from a seafloor borehole at the Juan de Fuca Ridge (Steinsbu et al, 2010).…”

Identification of key components in the energy metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus by transcriptome analyses

“…In A. fulgidus , this offers a potential coupling between ferredoxin and electron transport phosphorylation, but also represents a significant bioenergetic challenge, as fixation of CO 2 through the acetyl-CoA pathway requires Fd red . Interestingly, while chemoorganotrophic and carboxydotrophic growth are coupled to sulfate reduction in A. fulgidus , only thiosulfate or sulfite are utilized with H 2 as energy source (Stetter et al, 1987; Steinsbu et al, 2010). This may potentially be coupled to the role of Fd red in energy and carbon metabolism.…”