The Chinese communities' historical involvement in the architectural style of Malaysia's old mosques has established its presence in Malay society. Acculturation is focused on the meeting and mutual effect of Malay and Chinese cultures in this study. The goal of this research was to understand the aspects of Chinese culture that inspired the architectural and artistic styles of the mosques. This investigation focuses on the Masjid Kampung Keling in Malacca. The public's understanding of the history of Chinese cultural acculturation in this distinctive mosque is limited. As a result, this study was carried out to broaden and improve knowledge of traditional Malay architectural features, particularly Chinese acculturation. For this research work, a qualitative research methodology was developed alongside cultural studies and exploratory data methodologies in order to comprehend the mosque's distinct design aesthetic. The findings of this study provide a clearer picture of how early Islamic cultures in the Malay region developed their architecture. Pyramid roofs, roof crowns, embellishments, and towers are all examples of Chinese cultural acculturation. The findings of this study provided a better understanding of how earlier Muslim communities in the Malay world developed. As a result, it is expected that this study will provide a comprehensive picture of the evolution of mosque design in Malaysia and Malacca and can be applied to the ongoing development of concepts that will be useful to future academics.